<1> UI - 698CC-0001 DD - ISI Document Solution: 698CC AU - Garcia-Plazaola JI AU - Olano JM AU - Hernandez A AU - Becerril JM MA - gvpgaplj@lg.ehu.es RA - Garcia-Plazaola JI TI - Photoprotection in evergreen Mediterranean plants during sudden periods of intense cold weather SO - Trees-Structure & Function. 17(4):285-291, 2003 Jul. AS - Trees-Struct. Funct 2003 Jul;17(4):285-291 PU - SPRINGER-VERLAG, 175 FIFTH AVE, NEW YORK, NY 10010 USA. URL: http://www.springer-ny.com IS - 0931-1890 MH - Alpha-tocopherol MH - Antioxidants MH - Photooxidative stress MH - Xanthophyll cycle. MH - Xanthophyll cycle MH - Winter stress MH - Glutathione-reductase MH - Energy-dissipation MH - Seasonal-variation MH - Light energy MH - Photoinhibition MH - Photosynthesis MH - Antioxidants MH - Tocopherols. AB - The photoprotective responses to an abrupt period of exceptional cold weather were studied in several Mediterranean evergreen species with different ecological requirements. The same pattern of response was observed in most of the species with little change in hydrophilic antioxidants (ascorbate and glutathione) and the carotenoid pool, an increase in the content of alpha-tocopherol, and a night retention of de-epoxidised xanthophylls (antheraxanthin and zeaxanthin). The accumulation of these xanthophylls correlated with a sustained decrease in maximal photochemical efficiency (F-v/F-m). This reduction in the rate of electron transport would reduce the production of superoxide in photosystem I, as well as the subsequent hydrogen peroxide and hydroxyl radical. Thereby if any transitory photooxidative stress is produced under these conditions it should be due mainly to the formation of singlet oxygen by triplet excited chlorophyll within the antenna. Since alpha-tocopherol is the main scavenger of singlet oxygen and lipid peroxy radicals, the large increase of this antioxidant within the species could be enough to compensate for the higher degree of photooxidative stress, playing an essential role in the survival of vegetation during the incidence of exceptional cold fronts in the Mediterranean region. [References: 34] LG - English PT - Article SB - Current Contents(R)/Agriculture, Biology & Environmental Sciences CC - Plant Sciences in Current Contents(R)/Agricultural, Biology & Environmental Sciences. EW - 2003 week 31 IN - Reprint available from: Garcia-Plazaola JI Univ Pais Vasco, EHU, Dept Plant Biol & Ecol E-48080 Bilbao Spain Univ Pais Vasco, EHU, Dept Plant Biol & Ecol E-48080 Bilbao Spain Escuela Ingn Agrarias, Dept Ciencias Agroforestales, Area Biol Vegetal Soria 42003 Spain <2> UI - 695WV-0004 DD - ISI Document Solution: 695WV AU - Major JE AU - Barsi DC AU - Mosseler A AU - Campbell M AU - Rajora OP MA - jmajor@nrcan.gc.ca RA - Major JE TI - Light-energy processing and freezing-tolerance traits in red spruce and black spruce: species and seed-source variation SO - Tree Physiology. 23(10):685-694, 2003 Jul. AS - Tree Physiol 2003 Jul;23(10):685-694 PU - HERON PUBLISHING, 202, 3994 SHELBOURNE ST, VICTORIA, BC V8N 3E2, CANADA. URL: http://www.heronpublishing.com IS - 0829-318X MH - Adaptive traits MH - Chlorophyll fluorescence MH - Ecophysiology MH - Fitness MH - Genetic variation MH - Picea mariana MH - Picea rubens MH - Photosynthesis. MH - Chlorophyll fluorescence MH - Picea-rubens MH - Population viability MH - Genetic diversity MH - Frost hardiness MH - Cold hardiness MH - Douglas-fir MH - Seedlings MH - Photosynthesis MH - Dissipation. AB - Red spruce (Picea rubens Sarg.) and black spruce (Picea mariana (Mill.) B.S.P.) are genetically and morphologically similar but ecologically distinct species. We determined intraspecific seed-source and interspecific variation of red spruce and black spruce, from across the near-northern margins of their ranges, for several light-energy processing and freezing-tolerance adaptive traits. Before exposure to low temperature, red spruce had variable fluorescence (F-v) similar to black spruce, but higher photochemical efficiency (F-v/F-m), lower quantum yield, lower chlorophyll fluorescence (%), and higher thermal dissipation efficiency (q(N)), although the seed-source effect and the seed-source x species interaction were significant only for Fv/Fm. After low-temperature exposure (-40 degreesC), red spruce had significantly lower F-v/F-m, quantum yield and q(N) than black spruce, but higher chlorophyll, fluorescence and relative fluorescence. Species, seed-source effect, and seed-source x species interaction were consistent with predictions based on genetic (e.g., geographic) origins. Multi-temperature exposures (5, -20 and -40 degreesC) often produced significant species and temperature effects, and species x temperature interactions as a result of species-specific responses to temperature exposures. The inherent physiological species-specific adaptations of red spruce and black spruce were largely consistent with a shade-tolerant, late-successional species and an early successional species, respectively. Species differences in physiological adaptations conform to a biological trade-off, probably as a result of natural selection pressure in response to light availability and prevailing temperature gradients. [References: 40] LG - English PT - Article SB - Current Contents(R)/Agriculture, Biology & Environmental Sciences CC - Plant Sciences in Current Contents(R)/Agricultural, Biology & Environmental Sciences. EW - 2003 week 31 IN - Reprint available from: Major JE Nat Resources Cananda, Canadian Forestry Serv, Atlantic Forestry Ctr POB 4000 Fredericton NB E3B 5P7 Canada Nat Resources Cananda, Canadian Forestry Serv, Atlantic Forestry Ctr Fredericton NB E3B 5P7 Canada Dalhousie Univ, Dept Biol, Life Sci Ctr Halifax NS B3H 4J1 Canada <3> UI - 695WV-0006 DD - ISI Document Solution: 695WV AU - Senevirathna AMWK AU - Stirling CM AU - Rodrigo VHL MA - wasan@sltnet.lk RA - Senevirathna AMWK TI - Growth, photosynthetic performance and shade adaptation of rubber (Hevea brasiliensis) grown in natural shade SO - Tree Physiology. 23(10):705-712, 2003 Jul. AS - Tree Physiol 2003 Jul;23(10):705-712 PU - HERON PUBLISHING, 202, 3994 SHELBOURNE ST, VICTORIA, BC V8N 3E2, CANADA. URL: http://www.heronpublishing.com IS - 0829-318X MH - Chlorophyll fluorescence MH - Dynamic photoinhibition MH - Leaf area ratio MH - Leaf weight ratio MH - Relative growth rate MH - Shade-plants MH - Specific leaf area MH - Sun-plants. MH - High-light stress MH - Canopy photosynthesis MH - Leaf-area MH - Photoinhibition MH - Plants MH - Forest MH - Assimilation MH - Efficiency MH - Responses MH - Systems. AB - We compared growth, photosynthetic performance and shade adaptation of rubber (Hevea brasiliensis Muell. Arg.) plants growing in natural shade (33, 55 and 77% reduction in incoming radiation) to control plants growing in full sunlight. Stem diameter and plant height, measured over a 15-month period, were greatest in plants grown in full sunlight, and both parameters decreased with increasing shade. At 7 and 14 months after planting (MAP), total plant dry mass was highest in control plants and lowest in plants in 77% shade. Expansion of the fourth leaf whorl, monitored at 5-6 MAP, was slowest in plants in 77% shade and fastest in unshaded plants, which had more leaves and higher leaf areas and inter-whorl shoot lengths. In response to increasing shade, specific leaf area increased, whereas leaf weight ratio and relative growth rate decreased. Chlorophyll a/b ratio decreased with increasing shade, indicating shade-induced partitioning of chlorophyll into light-harvesting complexes. Compared to the response in unshaded plants, CO2 assimilation saturated at lower photosynthetic photon flux densities in plants in 77% shade, with a lower upper-asymptote to the light response curve. Chlorophyll fluorescence revealed no evidence of sustained photoinhibitory damage in unshaded plants. Dynamic photoinhibition decreased with increasing shade, with the greatest depression in the ratio of variable to maximal fluorescence around midday. We conclude that shade adaptation and shade-induced reductions in dynamic photoinhibition account for the enhanced early growth of rubber in light shade. [References: 40] LG - English PT - Article SB - Current Contents(R)/Agriculture, Biology & Environmental Sciences CC - Plant Sciences in Current Contents(R)/Agricultural, Biology & Environmental Sciences. EW - 2003 week 31 IN - Reprint available from: Senevirathna AMWK Rubber Res Inst Sri Lanka Dartonfield Agalawatta Sri Lanka Univ Coll N Wales, Sch Agr & Forest Sci Bangor LL57 2UW Gwynedd Wales <4> UI - 698EY-0004 DD - ISI Document Solution: 698EY AU - Sakiyan I AU - Yilmaz H MA - sakiyan@science.ankara.edu.tr RA - Sakiyan I TI - Manganese(III) complexes of some amino acid (L-serine, L-methionine, L-cystein) Schiff bases derived from 2-hydroxy-1-naphthaldehyde SO - Synthesis & Reactivity in Inorganic & Metal-Organic Chemistry. 33(6):971-983, 2003 Jul. AS - Synth. React. Inorganic Met.-Org. Chem 2003 Jul;33(6):971-983 PU - MARCEL DEKKER INC, 270 MADISON AVE, NEW YORK, NY 10016 USA. URL: http://www.dekker.com IS - 0094-5714 MH - Manganese complexes MH - Schiff bases of amino acids MH - Hydroxynaphthaldehyde. MH - Photosynthetic water oxidation MH - Photosystem-ii membranes MH - Pyridoxal analogs MH - Crystal-structure MH - Oxygen-evolution MH - Photoactivation MH - Spectra MH - Site MH - Mn. AB - A new series of manganese(III) complexes of amino acid Schiff bases synthesized from 2-hydroxy-1-naphthaldehyde and L-serine, L-methionine and L-cystein. The structure of their metal complexes was investigated by elemental analyses, IR, TGA, magnetic susceptibility, and mass spectra. The structure of the N-naphthylidene amino acid Schiff bases were determined by IR, UV, H-1 NMR and mass spectra for a comparison with those of the complexes. [References: 23] LG - English PT - Article SB - Current Contents(R)/Physical, Chemical & Earth Sciences CC - Inorganic & Nuclear Chemistry in Current Contents(R)/Physical, Chemical & Earth Sciences. EW - 2003 week 31 IN - Reprint available from: Sakiyan I Ankara Univ, Fac Sci, Dept Chem TR-06100 Ankara Turkey Ankara Univ, Fac Sci, Dept Chem TR-06100 Ankara Turkey <5> UI - 697CL-0014 DD - ISI Document Solution: 697CL AU - Boraldi F AU - Bini L AU - Liberatori S AU - Armini A AU - Pallini V AU - Tiozzo R AU - Pasquali-Ronchetti I AU - Quaglino D MA - quaglino.daniela@unimore.it RA - Quaglino D TI - Proteome analysis of dermal fibroblasts cultured in vitro from human healthy subjects of different ages SO - Proteomics. 3(6):917-929, 2003 Jun. AS - Proteomics 2003 Jun;3(6):917-929 PU - WILEY-V C H VERLAG GMBH, PO BOX 10 11 61, D-69451 WEINHEIM, GERMANY. URL: http://www.wiley-vch.de IS - 1615-9853 MH - Aging MH - Fibroblast MH - Mass spectrometry MH - Proteome MH - Two-dimensional gel electrophoresis. MH - 2-dimensional gel-electrophoresis MH - Human bj fibroblasts MH - Oxidative stress MH - Endoplasmic-reticulum MH - Polyacrylamide gels MH - Cellular senescence MH - Annexin-i MH - Life-span MH - Actin MH - Proteins. AB - Aging is a complex multifactorial process still far from being completely understood. The aim of the present study was to compare the proteome of in vitro cultured dermal fibroblasts from healthy subjects of different ages (i.e. 15 +/- 2, 41 +/- 4 and 82 +/- 3 years old). Proteins of the cell layer were separated by two-dimensional electrophoresis and protein identification was performed by matrix-assisted laser desorption/ionization time of flight mass spectrometry; moreover, synthetic gels were qualitatively and quantitatively analyzed by Melanie 3 software. Our study did not reveal any protein typical of any one age group. On the other hand, we observed 38 proteins exhibiting more than three-fold reproducible variations with aging, some (45%) being reduced such as F-actin capping protein alpha1, proteasome subunit alpha type 3, heat shock protein 27, ubiquitin carboxyl-terminal hydrolase isozyme L1, mitochondrial thioredoxin-dependent peroxide reductase, cathepsin B, glutathione S-transferase P, cyclophilin A and calgizzarin. In contrast, T-complex protein 1, probable protein disulfide isomerase ER60, phosphoglycerate kinase 1, Ran-specific GTPase-activating protein, proteasome subunit alpha type 5, triosephosphate isomerase and superoxide dismutase (Mn) increased with age. Furthermore, annexin 1, elongation factor 1beta, proteasome activator complex subunit 1, phosphoglycerate mutase, superoxide dismutase (Cu-Zn) and cofilin, exhibited the highest levels in adult cells; whereas, septin 2 homolog, RNA-binding protein regulatory subunit and ATP synthase D chain revealed the lowest values in adults. The present investigation, underlining the complexity of the aging process, highlights the role of synthetic and degradative pathways in modulating the whole cell machinery and emphasizes that metabolic impairment with age could depend partly on different expression of a number of genes and leading to an imbalance among functional proteins. [References: 76] LG - English PT - Article SB - Current Contents(R)/Life Sciences CC - Chemistry & Analysis in Current Contents(R)/Life Sciences. EW - 2003 week 31 IN - Reprint available from: Quaglino D Univ Modena, Dept Biomed Sci Via Campi 287 I-41100 Modena Italy Univ Modena, Dept Biomed Sci I-41100 Modena Italy Univ Siena, Dept Mol Biol I-53100 Siena Italy <6> UI - 697CL-0018 DD - ISI Document Solution: 697CL AU - Stulik J AU - Hernychova L AU - Porkertova S AU - Pozler O AU - Tuckova L AU - Sanchez D AU - Bures J MA - jstulik@pmfhk.cz RA - Stulik J TI - Identification of new celiac disease autoantigens using proteomic analysis SO - Proteomics. 3(6):951-956, 2003 Jun. AS - Proteomics 2003 Jun;3(6):951-956 PU - WILEY-V C H VERLAG GMBH, PO BOX 10 11 61, D-69451 WEINHEIM, GERMANY. URL: http://www.wiley-vch.de IS - 1615-9853 MH - Autoantigens MH - Celiac disease. MH - Alpha-enolase MH - Autoantibodies MH - Calreticulin MH - Epitopes. AB - Celiac disease is an autoimmune disorder in which gluten peptides presented by specific HLA-DQ2- and HLA-DQ8-positive antigen presenting cells elicit immune response in connective tissue of lamina propria. Immunoglobulin A (IgA) antiendomysial antibodies are specific for celiac disease and are used for screening, diagnosis and follow-up of this disease with an almost 100% sensitivity and specificity. The major target antigen of IgA antiendomysial antibodies was identified as tissue transglutaminase; nevertheless, the existence of the additional unique celiac disease-specific autoantigens is anticipated. In this study we have utilized a proteomic approach in order to search out new autoantigens recognized by serum antibodies of patients with active celiac disease. We report the detection of 11 proteins that were immunorecognized with various frequencies by sera of patients with celiac disease. Four autoantigens were identified by mass fingerprinting approach as actin, ATP synthase beta chain and two charge variants of enolase alpha. While production of IgA antibodies against actin the existence of autoantibodies to ATP synthase beta molecules were described earlier, chain and enolase a species in sera collected from patients with active celiac disease are described for the first time. These results are suggestive of the existence of additional celiac disease autoantigens with possible diagnostic utility. [References: 17] LG - English PT - Article SB - Current Contents(R)/Life Sciences CC - Chemistry & Analysis in Current Contents(R)/Life Sciences. EW - 2003 week 31 IN - Reprint available from: Stulik J Pukyne Mil Med Acad, Proteome Ctr Trebesska 1575 Hradec Kralove 50001 Czech Republic Pukyne Mil Med Acad, Proteome Ctr Hradec Kralove 50001 Czech Republic Univ Hosp, Dept Pediat Hradec Kralove Czech Republic Acad Sci Czech Republ, Inst Microbiol Prague Czech Republic Univ Hosp, Ctr Diagnost Hradec Kralove Czech Republic <7> UI - 695TE-0027 DD - ISI Document Solution: 695TE AU - Sapra R AU - Bagramyan K AU - Adams MWW MA - adams@bmb.uga.edu RA - Adams MWW TI - A simple energy-conserving system: Proton reduction coupled to proton translocation SO - Proceedings of the National Academy of Sciences of the United States of America. 100(13):7545-7550, 2003 Jun 24. AS - Proc. Natl. Acad. Sci. U. S. A 2003 Jun 24;100(13):7545-7550 PU - NATL ACAD SCIENCES, 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA. URL: http://www4.nationalacademies.org/nas/nashome.nsf IS - 0027-8424 MH - Archaeon pyrococcus-furiosus MH - Escherichia-coli MH - Complex-i MH - Ubiquinone oxidoreductase MH - Methanosarcina-barkeri MH - Rhodospirillum-rubrum MH - Nickel hydrogenases MH - Ech hydrogenase MH - Purification MH - Enzyme. AB - Oxidative phosphorylation involves the coupling of ATP synthesis to the proton-motive force that is generated typically by a series of membrane-bound electron transfer complexes, which ultimately reduce an exogenous terminal electron acceptor. This is not the case with Pyrococcus furiosus, an archaeon that grows optimally near 100degreesC. It has an anaerobic respiratory system that consists of a single enzyme, a membrane-bound hydrogenase. Moreover, it does not require an added electron acceptor as the enzyme reduces protons, the simplest of acceptors, to hydrogen gas by using electrons from the cytoplasmic redox protein ferredoxin. It is demonstrated that the production of hydrogen gas by membrane vesicles of A furiosus is directly coupled to the synthesis of ATP by means of a proton-motive force that has both electrochemical and pH components. Such a respiratory system enables rationalization in this organism of an unusual glycolytic pathway that was previously thought not to conserve energy. It is now clear that the use of ferredoxin in place of the expected NAD as the electron acceptor for glyceraldehyde 3-phosphate oxidation enables energy to be conserved by hydrogen production. In addition, this simple respiratory mechanism readily explains why the growth yields of P. furiosus are much higher than could be accounted for if ATP synthesis occurred only by substrate-level phosphorylation. The ability of microorganisms such as A furiosus to couple hydrogen production to energy conservation has important ramifications not only in the evolution of respiratory systems but also in the origin of life itself. [References: 37] LG - English PT - Article SB - Current Contents(R)/Life Sciences CC - Multidisciplinary in Current Contents(R)/Life Sciences. EW - 2003 week 31 IN - Reprint available from: Adams MWW Univ Georgia, Dept Biochem & Mol Biol, Ctr Metalloenzyme Studies Athens, GA 30602 USA Univ Georgia, Dept Biochem & Mol Biol, Ctr Metalloenzyme Studies Athens, GA 30602 USA Yerevan State Univ, Dept Biophys Yerevan 375049 Armenia <8> UI - 697DB-0028 DD - ISI Document Solution: 697DB AU - Henriques FS MA - ffh@mail.fct.unl.pt RA - Henriques FS TI - Gas exchange, chlorophyll a fluorescence kinetics and lipid peroxidation of pecan leaves with varying manganese concentrations SO - Plant Science. 165(1):239-244, 2003 Jul. AS - Plant Sci 2003 Jul;165(1):239-244 PU - ELSEVIER SCI IRELAND LTD, CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND. URL: http://www.elsevier.nl IS - 0168-9452 MH - Chlorophyll fluorescence MH - Gas exchange MH - Lipid peroxidation MH - Manganese deficiency. MH - Sugar-beet leaves MH - Spinach chloroplasts MH - Deficient spinach MH - Photosynthesis MH - Capacity MH - Toxicity MH - System MH - Stress MH - Plants. AB - Changes in pigment content, photosynthetic CO2 assimilation rate, photosystem II photochemical efficiency and lipid peroxidation of pecan [Carya illinoinensis (Wangenh.) C. Koch, cv. Kiowa] leaves induced by Mn deficiency were examined. Mn limitation was found to cause extensive declines in total chlorophyll and carotenoid contents in leaves, but it only slightly affected the chl a:b and chl:car ratios. Photosynthetic CO2 assimilation rates per unit leaf area decreased with the decreasing chlorophyll content in such a manner that the photosynthetic rates of Mn-deficient leaves were identical to those of controls when expressed on a chlorophyll weight basis. Chlorophyll fluorescence induction measurements showed that the ratio of variable to maximum (F-v:F-m) fluorescence underwent only small decreases with increasing manganese deficiency, indicating that the intrinsic quantum efficiency of the PSII units remaining in Mn-deprived leaves was not significantly decreased. Further, the net photosynthetic rates in Mn-deprived leaves were linearly correlated with the concentration of these remaining PSII units. Electron microscopy revealed no apparent change in the number and organization of thylakoids in chloroplasts from Mn-deficient leaves. From these results it is concluded that Mn deficiency depresses leaf photosynthetic capacity primarily by reducing the number of PSII units per unit leaf area, and that those remaining possess photochemical abilities similar to those of control leaves. It is also concluded that such decrease in PSII units results form a decrease in the number of chloroplasts per unit leaf area, rather from a decrease in the number of PSIIs per individual chloroplast. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved. [References: 35] LG - English PT - Article SB - Current Contents(R)/Agriculture, Biology & Environmental Sciences Current Contents(R)/Life Sciences CC - Plant Sciences in Current Contents(R)/Agricultural, Biology & Environmental Sciences. Animal & Plant Sciences in Current Contents(R)/Life Sciences. EW - 2003 week 31 IN - Reprint available from: Henriques FS Univ Nova Lisboa, Fac Ciencias & Tecnol, Plant Biol Unit, Grp Disciplinas Biol Vegetal P-2829516 Caparica Portugal Univ Nova Lisboa, Fac Ciencias & Tecnol, Plant Biol Unit, Grp Disciplinas Biol Vegetal P-2829516 Caparica Portugal <9> UI - 697DB-0030 DD - ISI Document Solution: 697DB AU - Yamazaki J AU - Ohashi A AU - Hashimoto Y AU - Negishi E AU - Kumagai S AU - Kubo T AU - Oikawa T AU - Maruta E AU - Kamimura Y MA - junya@bio.sci.toho-u.ac.jp RA - Yamazaki J TI - Effects of high light and low temperature during harsh winter on needle photodamage of Abies mariesii growing at the forest limit on Mt. Norikura in Central Japan SO - Plant Science. 165(1):257-264, 2003 Jul. AS - Plant Sci 2003 Jul;165(1):257-264 PU - ELSEVIER SCI IRELAND LTD, CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND. URL: http://www.elsevier.nl IS - 0168-9452 MH - Forest limit MH - Psii down-regulation MH - Water-water cycle MH - Winter stress MH - Xanthophyll cycle MH - Abies mariesu. MH - Pigment-binding protein MH - Photosystem-ii MH - Scots pine MH - Photoinhibition MH - Plants MH - Photosynthesis MH - Acclimation MH - Carotenoids MH - Stress MH - Energy. AB - Coniferous evergreen firs Abies mariesii (A. mariesii) growing at the forest limit (near 2500 m altitude) on Mt. Norikura (36degrees61'N, 137degrees33'E, 3026 in altitude) in Central Japan are exposed to severe winter-stresses. Under such conditions, only the abaxial side of A. mariesii needles changes from green to reddish-brown in early spring, resulting in the death of the needles. Since this needle damage is only observed in shoots that protrude from the snow surface and not in those buried in snow or located at lower elevations, this phenomenon seems to be caused by the interaction of strong sunlight reflected from the snow surface and low temperature. We found that the damaged needles have increased in the de-epoxidation state of xanthophyll cycle because they contained large amounts of zeaxanthin, which appears when the leaves encounter a strong light stress, leading to the long-term down-regulation of PS II photochemistry. These results indicate that the needles acclimatize against the strong light during severe winter. Furthermore, ascorbate peroxidase (APX, E.C. 1.11.1.11) activity was found to decrease remarkably during critical subzero periods, while superoxide dismutase (SOD, E.C. 1.15.1.1) activity remains constant throughout the year. Based on these results, we discuss herein how A. mariesii growing at the forest limit of temperate zone responds to high light and low temperature in harsh winter conditions. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved. [References: 29] LG - English PT - Article SB - Current Contents(R)/Agriculture, Biology & Environmental Sciences Current Contents(R)/Life Sciences CC - Plant Sciences in Current Contents(R)/Agricultural, Biology & Environmental Sciences. Animal & Plant Sciences in Current Contents(R)/Life Sciences. EW - 2003 week 31 IN - Reprint available from: Yamazaki J Toho Univ, Fac Sci, Dept Biol Miyama 2-2-1 Chiba 2748510 Japan Toho Univ, Fac Sci, Dept Biol Chiba 2748510 Japan Univ Tsukuba, Grad Sch Environm Sci Tsukuba Ibaraki 3058572 Japan Univ Tsukuba, Inst Biol Sci Tsukuba Ibaraki 3058572 Japan <10> UI - 696YH-0002 DD - ISI Document Solution: 696YH AU - Losi A AU - Braslavsky SE MA - braslavskys@mpi-muelheim.mpg.de RA - Braslavsky SE TI - The time-resolved thermodynamics of the chromophore-protein interactions in biological photosensors as derived from photothermal measurements [Review] SO - Physical Chemistry Chemical Physics. 5(13):2739-2750, 2003. AS - Phys. Chem. Chem. Phys 2003;5(13):2739-2750 PU - ROYAL SOC CHEMISTRY, THOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLAND. URL: http://www.rsc.org IS - 1463-9076 MH - Photoactive yellow protein MH - Sensory rhodopsin-ii MH - Blue-light receptors MH - Phytochrome-a phototransformation MH - Photosynthetic reaction centers MH - Entropy-enthalpy compensation MH - Electron-transfer reactions MH - Plant photoreceptor domain MH - Bacterial reaction centers MH - Structural volume changes. AB - Laser-induced optoacoustic spectroscopy, LIOAS, and photothermal beam deflection, PBD, were applied to the determination of the time ( nanosecond to millisecond) resolved enthalpy and structural volume changes photoinduced in various biological photosensors and in the phototriggered bacterial ion pump halorhodopsin. A brief section introduces the various photosensors, several of them recently discovered. The photosensors studied with photothermal methods ( LIOAS and PBD) have either a chromophore with an isomerizable double bond, such as phytochrome A of etiolated plants, sensory rhodopsins of Archaea, and photoactive yellow protein of Eubacteria, or an FMN (flavin mononucleotide) chromophore, such as the LOV ( light, oxygen, or voltage) sensing domains of phototropins and related proteins. In particular cases the data allow the evaluation of the entropy changes produced in some of the photoinduced steps, a quantity not available by other methods. In some cases UV-vis spectroscopically silent transient species could be observed. The molecular origin of the structural volume changes is analysed. The problems involved in the application of these techniques to several photosensors are addressed, such as their time limits and the determination of the absorption-determined refractive index changes in PBD. [References: 100] LG - English PT - Review SB - Current Contents(R)/Physical, Chemical & Earth Sciences CC - Physical Chemistry/Chemical Physics in Current Contents(R)/Physical, Chemical & Earth Sciences. EW - 2003 week 31 IN - Reprint available from: Braslavsky SE Max Planck Inst Strahlenchem Postfach 10 13 65 D-45413 Mulheim Germany Max Planck Inst Strahlenchem D-45413 Mulheim Germany <11> UI - 696YY-0001 DD - ISI Document Solution: 696YY AU - Wozniak B AU - Dera J AU - Ficek D AU - Majchrowski R AU - Ostrowska M AU - Kaczmarek S MA - wozniak@iopan.gda.pl RA - Wozniak B TI - Modelling light and photosynthesis in the marine environment [Review] SO - Oceanologia. 45(2):171-245, 2003. AS - Oceanologia 2003;45(2):171-245 PU - POLISH ACAD SCIENCES INST OCEANOLOGY, POWSTANCOW WASZAWY 55, PL-81-712 SOPOT, POLAND. URL: http://www.iopan.gda.pl IS - 0078-3234 MH - Marine environment MH - Underwater irradiance MH - Bio-optical modelling MH - Light absorption MH - Pigments MH - Photo- and chromatic acclimation MH - Photosynthesis quantum yield MH - Primary production MH - Remote sensing algorithm. MH - Chlorophyll-a fluorescence MH - Western north-atlantic MH - Quantum yield MH - Steady-state MH - Phytoplankton photosynthesis MH - Optical characteristics MH - Natural variations MH - Spectral model MH - Upper ocean MH - Absorption. AB - The overriding and far-reaching aim of our work has been to achieve a good understanding of the processes of light interaction with phytoplankton in the sea and to develop an innovative physical model of photosynthesis in the marine environment, suitable for the remote sensing of marine primary production. Unlike previous models, the present one takes greater account of the complexity of the physiological processes in phytoplankton. We have focused in particular on photophysiological processes, which are governed directly or indirectly by light energy, or in which light, besides the nutrient content in and the temperature of seawater, is one of the principal limiting factors. To achieve this aim we have carried out comprehensive statistical analyses of the natural variability of the main photophysiological properties of phytoplankton and their links with the principal abiotic factors in the sea. These analyses have made use of extensive empirical data gathered in a wide diversity of seas and oceans by Polish and Russian teams as well as by joint Polish-Russian expeditions. Data sets available on the Internet have also been applied. As a result, a set of more or less complex, semi-empirical models of light-stimulated processes occurring in marine phytoplankton cells has been developed. The trophic type of sea, photo-acclimation and the production of photoprotecting carotenoids, chromatic acclimation and the production of various forms of chlorophyll-antennas and photosynthetic carotenoids, cell adaptation by the package effect, light absorption, photosynthesis, photoinhibition, the fluorescence effect, and the activation of PS2 centres are all considered in the models. These take into account not only the influence of light, but also, indirectly, that of the vertical mixing of water; in the case of 'photosynthesis, the quantum yield has been also formulated as being dependent on the nutrient concentrations and the temperature of seawater. The bio-optical spectral models of irradiance transmittance in case 1 oceanic waters and case 2 Baltic waters, developed earlier, also are described in this paper. The development of the models presented here is not yet complete and they all need continual improvement. Nevertheless, we have used them on a preliminary basis for calculating various photosynthetic characteristics at different depths in the sea, such as the concentration of chlorophyll and other pigments, and primary production. The practical algorithm we have constructed allows the vertical distribution of these characteristics to be determined from three input data: chlorophyll a concentration, irradiance, and temperature at the sea surface. Since all three data can be measured remotely, our algorithm can be applied as the 'marine part' of the remote sensing algorithms used for detecting marine photosynthesis. [References: 161] LG - English PT - Review SB - Current Contents(R)/Agriculture, Biology & Environmental Sciences CC - Aquatic Sciences in Current Contents(R)/Agricultural, Biology & Environmental Sciences. EW - 2003 week 31 IN - Reprint available from: Wozniak B Polish Acad Sci Powstancow Warszawy 55 PL-81712 Sopot Poland Polish Acad Sci PL-81712 Sopot Poland Pomeranian Pedag Acad Slupsk, Inst Phys PL-76200 Slupsk Poland <12> UI - 697DZ-0004 DD - ISI Document Solution: 697DZ AU - Hisabori T AU - Ueoka-Nakanishi H TI - ATP synthase: Molecular mechanism of rotation and regulation [Japanese] SO - Nippon Nogeikagaku Kaishi-Journal of the Japan Society for Bioscience Biotechnology & Agrochemistry. 77(7):659-662, 2003. AS - Nippon Nogeikagaku Kaishi-J. Jpn. Soc. Biosci. Biotechol. Agrochem 2003;77(7):659-662 PU - JAPAN SOC BIOSCI BIOTECHN AGROCHEM, JAPAN ACAD SOC CTR BLDG, 2-4-6 YAYOI BUNKYO-KU, TOKYO, 113, JAPAN. URL: http://wwwsoc.nacsis.ac.jp/jsbba IS - 0002-1407 MH - Atp synthase MH - Chloroplast MH - Redox regulation MH - Rotation. MH - Gamma-subunit MH - Escherichia-coli MH - F-1-atpase. LG - Japanese PT - Article SB - Current Contents(R)/Agriculture, Biology & Environmental Sciences CC - Agricultural Chemistry in Current Contents(R)/Agricultural, Biology & Environmental Sciences. EW - 2003 week 31 <13> UI - BW97U-0035 DD - ISI Document Solution: BW97U AU - Fernandes MAS AU - Santos MS AU - Alpoim MC AU - Madeira VMC AU - Vicente JAF RA - Fernandes MAS TI - Chromium interference with mitochondrial bioenergetics ED - Khassanova L, Collery P, Maymard I, Khassanova Z, Etienne JC SO - METAL IONS IN BIOLOGY AND MEDICINE, VOL 7. 7 PG. 163-167. 2002 [Figures], [Plates]. AS - METAL IONS IN BIOLOGY AND MEDICINE, VOL 7 2002;7:163-167 PU - JOHN LIBBEY EUROTEXT, 127 AVE DE LA REPUBLIQUE, 92120 MONTROUGE, FRANCE. URL: http://www.john/libbey/eurotext.fr ST - METAL IONS IN BIOLOGY AND MEDICINE BI - JOHN LIBBEY EUROTEXT, JACQUES GROSSET, 127 AVE DE LA REPUBLIQUE, 92120 MONTROUGE, FRANCE, Softbound: ISBN 2-7420-0429-7, 95.00 (EUROPEAN CURRENCY). (Prepayment Required), , MAKE CHECK PAYABLE TO: JOHN LIBBEY EUROTEXT, 127 AVE DE LA REPUBLIQUE, 92120 MONTROUGE, FRANCE TEL:(33)01 46 73 01 32, FX:(33) 01 46 73 06 73 OR CONTACT:WWW.JOHN-LIBBEY-EUROTEXT.FR MH - Respiration MH - Cr(vi) MH - Stress MH - Cells. AB - The mechanism of Cr(VI)-induced toxicity in plants and animals was studied for mitochondrial bioenergetics in turnip root and rat liver mitochondria. Chromium(VI) interference with mitochondrial bionergetics of plant and animal mitochondria was shown by decrease of the succinate-dependent respiratory indexes, (RCR and ADP/O); alterations on oxygen-supported respiration by succinate on state 4, state 3 and uncoupled respiration with parallel AT dissipation and decrease of the phosphorylation efficiency; inhibition of the NADH-ubiquinone oxidoreductase (complex I) and succinate-dehydrogenase (complex II); alterations on the mitochondrial ATPase activity (complex V); and membrane permeabilization to K+. Although Cr(VI) interferes with mitochondrial bionergetics of both plant and animal mitochondria, differences on the sensitivity to Cr(VI), AT and phosphorylation efficiency, state 4 respiration and ATPase activities, and K+ influx were detected between them. In conclusion, CrVI)-induced perturbations on mitochondrial bioenergetics may contribute to the basal mechanism underlying its toxic effects in plant and animal cells. [References: 13] LG - English PT - Article SB - Current Contents(R)/Life Sciences CC - Current Book Contents in Current Contents(R)/Life Sciences. EW - 2003 week 31 IN - Reprint available from: Fernandes MAS Dept Zool P-3004517 Coimbra Portugal Dept Zool P-3004517 Coimbra Portugal <14> UI - BW97U-0052 DD - ISI Document Solution: BW97U AU - Gogotov IN AU - Zorin NA AU - Tikhonov KG RA - Gogotov IN TI - Biosorption of metal ions by microorganisms and their consortia with aqueous plants ED - Khassanova L, Collery P, Maymard I, Khassanova Z, Etienne JC SO - METAL IONS IN BIOLOGY AND MEDICINE, VOL 7. 7 PG. 247-251. 2002 [Figures], [Plates]. AS - METAL IONS IN BIOLOGY AND MEDICINE, VOL 7 2002;7:247-251 PU - JOHN LIBBEY EUROTEXT, 127 AVE DE LA REPUBLIQUE, 92120 MONTROUGE, FRANCE. URL: http://www.john/libbey/eurotext.fr ST - METAL IONS IN BIOLOGY AND MEDICINE BI - JOHN LIBBEY EUROTEXT, JACQUES GROSSET, 127 AVE DE LA REPUBLIQUE, 92120 MONTROUGE, FRANCE, Softbound: ISBN 2-7420-0429-7, 95.00 (EUROPEAN CURRENCY). (Prepayment Required), , MAKE CHECK PAYABLE TO: JOHN LIBBEY EUROTEXT, 127 AVE DE LA REPUBLIQUE, 92120 MONTROUGE, FRANCE TEL:(33)01 46 73 01 32, FX:(33) 01 46 73 06 73 OR CONTACT:WWW.JOHN-LIBBEY-EUROTEXT.FR AB - Phototrophic and chemotrophic microorganisms of different taxonomic groups and their consortia with water plants (aquatic fern Azolla, water hyacinth) are capable to accumulate metal ions of Ni, Pt, Ru, Cu, Cr, Pb, Zn, Si and Au. This ability enables to use them for purification of agricultural and industrial waste water from toxic heavy metals and to obtain rare trace metals. For this purpose it is possible to use growing cultures and immobilized cells, their biopolymers or enzymes (hydrogenase). Purple non-sulfur bacteria (Rhodobacter spp., Rhodopseudomonas spp.) are able to accumulate Cu, Zn, Ni and Hg., showing various resistance to these metals. Biopolymer of purple sulfur bacterium Ectothiorhodospira shaposhnikovii accumulated more than 99% Cu and Zn from diluted waste water of electrolysis. Green algae Chlorella spp. and water plants Azolla, duckweed and water hyacinth showed higher ability to a biosorption of metal ioins. Cyanobacteria (Spirulina platensis) and plants (amaranthus) may be recommend as producers of homeopathic drugs containing necessary metals (Se, Zn, Ni etc.) for microelemental diseases. Many of metal ions play the important role in a metabolism of microorganisms, plants, animals and man [1, 2]. They are required for synthesis of pigments, electron carriers, enzymes, vitamins and other cell components. Elevated concentrations or deficit of a number of metal ions induce a toxic effect on organisms, causing various diseases or suppressing their growth. Chemotrophic and phototrophic microorganisms, as well as their consortia with plants and lichens, accumulate efficiently the metals from an environment in the amount higher their requirements for growth, that can be used for bioremediation of waste water and soils, and for the development of technologies of obtaining dissipated expensive metals and homeopathic drugs as well [1-4]. The amount of metals accumulated by them can be different. The absorption mechanisms also vary from simple physicochemical process of absorption and desorption, up to highly specific systems of a transport of metal ions in the cells. More than 16 metals are necessary for the growth of microorganisms and plants and their metabolism. However, biological functions of many metals and mechanism of their operation are not clear. The, purpose of this presentation is the analysis of the literary and own data on efficiency of a biosorption and stability to ions of metals of the growing cultures, suspensions of native (immobilized) cells, their biopolymers and enzymes. [References: 10] LG - English PT - Article SB - Current Contents(R)/Life Sciences CC - Current Book Contents in Current Contents(R)/Life Sciences. EW - 2003 week 31 IN - Reprint available from: Gogotov IN RAS, Inst Basic Biol Problems Pushchino 142290 Russia RAS, Inst Basic Biol Problems Pushchino 142290 Russia <15> UI - BW97U-0086 DD - ISI Document Solution: BW97U AU - Moalem S AU - Percy ME AU - Kruck T AU - Gelbart RR RA - Moalem S TI - Could epidemic pathogenic selection (EPS) be an explanation for hereditary hemochromatosis? ED - Khassanova L, Collery P, Maymard I, Khassanova Z, Etienne JC SO - METAL IONS IN BIOLOGY AND MEDICINE, VOL 7. 7 PG. 415-417. 2002 [Figures], [Plates]. AS - METAL IONS IN BIOLOGY AND MEDICINE, VOL 7 2002;7:415-417 PU - JOHN LIBBEY EUROTEXT, 127 AVE DE LA REPUBLIQUE, 92120 MONTROUGE, FRANCE. URL: http://www.john/libbey/eurotext.fr ST - METAL IONS IN BIOLOGY AND MEDICINE BI - JOHN LIBBEY EUROTEXT, JACQUES GROSSET, 127 AVE DE LA REPUBLIQUE, 92120 MONTROUGE, FRANCE, Softbound: ISBN 2-7420-0429-7, 95.00 (EUROPEAN CURRENCY). (Prepayment Required), , MAKE CHECK PAYABLE TO: JOHN LIBBEY EUROTEXT, 127 AVE DE LA REPUBLIQUE, 92120 MONTROUGE, FRANCE TEL:(33)01 46 73 01 32, FX:(33) 01 46 73 06 73 OR CONTACT:WWW.JOHN-LIBBEY-EUROTEXT.FR MH - Coenzyme q(10) level MH - Pediatric population MH - Acute inflammatory process. MH - Protein MH - Resistance MH - Disease MH - Nramp1 MH - Hfe. AB - Using a newly developed metod by HPLC with ultraviolet detection we measured plasma coenzyme Q(10) (CoQ(10)) le vel in group of 43 children (19 femals and 24 males: ages 1 month - 9 years) with an acute inflammatory process. The results for coenzyme Q(10) were expressed as molar concentration (mumol/l plasma). Our study confirmed that CoQ(10) concentration (median 0.8 mumol/l) was independent of sex, and we established which biochemical parameters influence on ubiquinone levels. The results indicate that CoQ(10) concentration is connected with leukocytosis, calcium and magnesium levels. These findings suggested that transferin, amylase and serum glutamic transaminase may also determine the CoQ(10) levels plasma. [References: 13] LG - English PT - Article SB - Current Contents(R)/Life Sciences CC - Current Book Contents in Current Contents(R)/Life Sciences. EW - 2003 week 31 IN - Reprint available from: Moalem S Univ Toronto, Dept Physiol Med Sci Bldg Toronto ON M5S 1A8 Canada Univ Toronto, Dept Physiol Toronto ON M5S 1A8 Canada <16> UI - BW97U-0088 DD - ISI Document Solution: BW97U AU - Mikoluc B AU - Karpinska J AU - Motkowski R AU - Piotrowska-Jastrzebska J RA - Mikoluc B TI - Coenzyme Q(10) level in plasma of children with inflammatory process ED - Khassanova L, Collery P, Maymard I, Khassanova Z, Etienne JC SO - METAL IONS IN BIOLOGY AND MEDICINE, VOL 7. 7 PG. 421-426. 2002 [Figures], [Plates]. AS - METAL IONS IN BIOLOGY AND MEDICINE, VOL 7 2002;7:421-426 PU - JOHN LIBBEY EUROTEXT, 127 AVE DE LA REPUBLIQUE, 92120 MONTROUGE, FRANCE. URL: http://www.john/libbey/eurotext.fr ST - METAL IONS IN BIOLOGY AND MEDICINE BI - JOHN LIBBEY EUROTEXT, JACQUES GROSSET, 127 AVE DE LA REPUBLIQUE, 92120 MONTROUGE, FRANCE, Softbound: ISBN 2-7420-0429-7, 95.00 (EUROPEAN CURRENCY). (Prepayment Required), , MAKE CHECK PAYABLE TO: JOHN LIBBEY EUROTEXT, 127 AVE DE LA REPUBLIQUE, 92120 MONTROUGE, FRANCE TEL:(33)01 46 73 01 32, FX:(33) 01 46 73 06 73 OR CONTACT:WWW.JOHN-LIBBEY-EUROTEXT.FR MH - Coenzyme q(10) level MH - Pediatric population MH - Acute inflammatory process. MH - Cancer. AB - Using a newly developed metod by HPLC with ultraviolet detection we measured plasma coenzyme Q(10) (CoQ(10)) level in group of 43 children (19 fernals and 24 males: ages 1 month 9 years) with an acute inflammatory process. The results for coenzyme Q(10) were expressed as molar concentration (mumol/l plasma). Our study confirmed that CoQ(10) concentration (median 0.8 mumol/l) was independent of sex, and we established which biochemical parameters influence on ubiquinone levels. The results indicate that CoQ(10) concentration is connected with leukocytosis, calcium and magnesium levels. These findings suggested that transferin, amylase and serum glutamic transaminase may also determine the CoQ(10)-levels plasma. [References: 11] LG - English PT - Article SB - Current Contents(R)/Life Sciences CC - Current Book Contents in Current Contents(R)/Life Sciences. EW - 2003 week 31 IN - Reprint available from: Mikoluc B Med Acad Bialystok, Dept Propedeut Pediat Ul J Waszyngtona 17 PL-15274 Bialystok Poland Med Acad Bialystok, Dept Propedeut Pediat PL-15274 Bialystok Poland <17> UI - 696LD-0005 DD - ISI Document Solution: 696LD AU - Rijstenbil JW MA - j.rijstenbil@nioo.knaw.nl RA - Rijstenbil JW TI - Effects of UVB radiation and salt stress on growth, pigments and antioxidative defence of the marine diatom Cylindrotheca closterium SO - Marine Ecology-Progress Series. 254:37-47, 2003. AS - Mar. Ecol.-Prog. Ser 2003;254:37-47 PU - INTER-RESEARCH, NORDBUNTE 23, D-21385 OLDENDORF LUHE, GERMANY. URL: http://www.int-res.com IS - 0171-8630 MH - Antioxidants MH - Cylindrotheca closterium MH - Diatom MH - Pigments MH - Salt stress MH - Ultraviolet. MH - Ultraviolet-b radiation MH - Hydrogen-peroxide formation MH - Oxidative stress MH - Chlorophyll fluorescence MH - Elevated-temperatures MH - Peridinium-gatunense MH - Benthic diatom MH - Primary leaves MH - Lake kinneret MH - Fresh-water. AB - During emersion, diatoms are exposed to sunlight (with UVB) on tidal flats, where the salinity may increase as a result of evaporation. In order to investigate whether a combination of UV radiation and desiccation would cause oxidative stress under such conditions, an experiment has been carried out with the diatom Cylindrotheca closterium. Cell division rates, photosynthetic efficiencies, pigment contents, and activities of antioxidant enzymes (SOD, APX, MDHAR, GR) were analysed in exponential-phase batch cultures grown at 35 (normal salinity) and 70 PSU (hyperosmotic) in a 12 h: 12 h light:dark cycle (268 mumol photons m(-2) s(-1) photosynthetically active radiation; PAR) at 15degreesC. Within the 12 h photoperiod, UVA (2.80 W m(-2) unweighted) or UVA+UVB (4.23 and 0.22 W m(-2), respectively) were supplemented during 4 h. Separately as well as in combination, UVB (3.45 kJ m(-2) d(-1)) and salt stress (70 PSU) caused a decrease in division rates. This UVB dose caused a decrease in photosynthetic efficiency, whereas salt stress did not. Cell volumes of UVB-exposed C. closterium increased only at 70 PSU. UVB radiation and salt stress caused significant decreases in chl a, chl c and fucoxanthin contents, but the UVB effect was stronger than the salt effect. The relatively high beta-carotene:chl a ratios in UVB-exposed and salt-stressed cells might indicate that P-carotene was used in scavenging singlet oxygen. In high light with UVA (at 35 PSU), SOD, APX, MDHAR and GR activities were not higher than in low light (27 mumol photons m(-2) s(-1)). Separately and in combination, UVB and salt stress enhanced SOD activity in C. closterium, whereas APX was stimulated by UVB only. MDHAR was stimulated under UVB and salt stress, but there were no effects on GR activity. Under ambient salt and UV conditions during emersion, oxidative stress may contribute to an inhibition of growth of C. closterium. [References: 63] LG - English PT - Article SB - Current Contents(R)/Agriculture, Biology & Environmental Sciences CC - Aquatic Sciences in Current Contents(R)/Agricultural, Biology & Environmental Sciences. EW - 2003 week 31 IN - Reprint available from: Rijstenbil JW NIOO KNAW, CEME POB 140 NL-4400 AC Yerseke Netherlands NIOO KNAW, CEME NL-4400 AC Yerseke Netherlands <18> UI - 698EF-0006 DD - ISI Document Solution: 698EF AU - Ouzounidou G AU - Ilias I AU - Kabataidis M AU - Chatzimichail A MA - geouz@nagref.gr RA - Ouzounidou G TI - Comparative study of nutrient deficiencies on growth and photochemistry of tobacco SO - Journal of Plant Nutrition. 26(8):1605-1616, 2003. AS - J. Plant Nutr 2003;26(8):1605-1616 PU - MARCEL DEKKER INC, 270 MADISON AVE, NEW YORK, NY 10016 USA. URL: http://www.dekker.com IS - 0190-4167 MH - Chlorophyll fluorescence MH - Electron transport MH - Nicotiana tabacum MH - Nutrient deficiency. MH - Chlorophyll fluorescence MH - Photosynthetic apparatus MH - Oxygen evolution MH - Photosystem-ii MH - Chloroplasts MH - Physiology MH - Membranes MH - Calcium MH - Plants MH - Leaves. AB - Element deficiencies, such as molybdenum (Mo), iron (Fe), calcium (Ca), lead to disturbances of morphological and physiological processes of tobacco (Nicotiana tabacum L.). On exposure to nutrient solution without Mo, leaf morphology was significantly affected, whereas photosynthetic processes conserved their normal activities. The decreased Mo, Fe, and Ca concentrations in tobacco were associated with loss of pigments and photosynthetic disturbances. In Ca-deficient plants a reduction in the rate constant of energy trapping by PSII centers and a physical dissociation of LHC from PSII core, were observed. The poisonous action of Fe and Ca deficiencies focused mainly in the decreased proportion of active chlorophyll associated with the reaction center (RC) of PSII (decreased F-v/F-0) and in the declined total number of electrons that have gone through the RCs (decreased Area/F-m - F-0). In parallel, the non-photochemical quenching coefficient was significantly enhanced in Fe and Ca-deficient plants, but remained unchanged in Mo-deficient plants. It is obvious that nutrient deficiency may trigger some protective mechanisms in order to PSII could maintain its activity under that type of stress. [References: 26] LG - English PT - Article SB - Current Contents(R)/Agriculture, Biology & Environmental Sciences CC - Plant Sciences in Current Contents(R)/Agricultural, Biology & Environmental Sciences. EW - 2003 week 31 IN - Reprint available from: Ouzounidou G Natl Agr Res Fdn, Inst Food Technol Lycovrissi 14123 Greece Natl Agr Res Fdn, Inst Food Technol Lycovrissi 14123 Greece Technol Educ Inst Thessaloniki, Fac Agr Technol, Dept Plant Protect Sindos Greece <19> UI - 697PB-0008 DD - ISI Document Solution: 697PB AU - Mouritsen LT AU - Richardson K MA - richardson@biology.au.dk RA - Richardson K TI - Vertical microscale patchiness in nano- and microplankton distributions in a stratified estuary SO - Journal of Plankton Research. 25(7):783-797, 2003 Jul. AS - J. Plankton Res 2003 Jul;25(7):783-797 PU - OXFORD UNIV PRESS, GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND. URL: http://www.oup.co.uk IS - 0142-7873 MH - Ciliate mesodinium-rubrum MH - Marine-phytoplankton MH - Light-intensity MH - Baltic sea MH - Migration MH - Plankton MH - Dinophysis MH - Coastal MH - Chlorophyll MH - Limitation. AB - Microscale (decimetre) vertical heterogeneity in the distribution of nano- and microplankton was studied on August 23, 1999 at two different sites (separated by similar to40 m) in Aarhus Bay, Denmark. At the time of sampling, the water column was stratified with respect to both temperature and salinity and a subsurface fluorescence maximum (corresponding to similar to10 mug chlorophyll a l(-1)) occurred immediately below the primary pycnocline (9 m). Samples for species identification were taken at the surface, near the bottom of the water column and at 20 15-cm intervals in and around the depth of maximum fluorescence. The plankton communities recorded in the three different regions of the water column differed dramatically from one another. In addition, significant differences were found in the distribution patterns of species and functional groups in the region of sampling around the fluorescence peak. The same patterns in vertical species distributions were observed at the two stations. In the region surrounding the subsurface fluorescence peak, diatoms were, generally, regularly distributed, although the total diatom biomass decreased slightly with depth. Dinoflagellate species were mainly non-regularly distributed and could be divided into two groups: (i) autotrophic or potentially mixotrophic species (Dinophysis norvegica, Dinophysis acuminata, Dinophysis cf. dens, Prorocentrum micans, Gymnodinium chlorophorum and Ceratium macroceros) that mainly decreased in numbers with depth or were aggregated in their distribution; and (ii) heterotrophic or potentially mixotrophic species (Ceratium lineatum, Ceratium longipes, Ceratium furca, Ceratium tripos, Ceratium fusus, Protoperidinium curtipes, Protoperidinium steinii, Diplopsalis spp. and Katodinium glaucum). In the latter group, the species mainly increased in numbers with depth or were randomly distributed. Most ciliates were uniformly distributed vertically in the water column. However, small cells of the photosynthesizing ciliate Mesodinium rubrum were most abundant at the depth of maximal fluorescence while large M. rubrum cells were equally abundant at all the depths sampled, suggesting the two size groups of this organism may differ ecologically. Overall, the study demonstrates that traditional plankton sampling methods may lead to misinterpretations of speciesco-existence and interactions. [References: 50] LG - English PT - Article SB - Current Contents(R)/Agriculture, Biology & Environmental Sciences CC - Aquatic Sciences in Current Contents(R)/Agricultural, Biology & Environmental Sciences. EW - 2003 week 31 IN - Reprint available from: Richardson K Aarhus Univ, Inst Biol, Dept Marine Ecol Finlandsgade 14 DK-8200 Aarhus N Denmark Aarhus Univ, Inst Biol, Dept Marine Ecol DK-8200 Aarhus N Denmark <20> UI - 697PB-0009 DD - ISI Document Solution: 697PB AU - Richardson K AU - Rasmussen B AU - Bunk T AU - Mouritsen LT MA - richardson@biology.au.dk RA - Richardson K TI - Multiple subsurface phytoplankton blooms occurring simultaneously in the Skagerrak SO - Journal of Plankton Research. 25(7):799-813, 2003 Jul. AS - J. Plankton Res 2003 Jul;25(7):799-813 PU - OXFORD UNIV PRESS, GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND. URL: http://www.oup.co.uk IS - 0142-7873 MH - Photosynthetic energy-conversion MH - North-sea MH - Community structure MH - Plankton community MH - Quantum yield MH - Dogger bank MH - Nitrogen MH - Variability MH - Limitation MH - Chlorophyll. AB - The distribution and characteristics of phytoplankton in the Skagerrak in August-September 2000 were analysed in order to evaluate the importance of subsurface phytoplankton peaks to water column ecology and primary production. In areas affected by outflow from the Baltic, enhanced chlorophyll concentrations were found in the warm surface waters (i.e. upper 10-20 m). However, for the central Skagerrak, the major part (50-80%) of the chlorophyll in the water column was found below the warm surface waters. The highest chlorophyll concentrations (up to >18 mug l(-1)) in the study area were also found below the warm surface waters and up to 95% of total water column primary production was recorded below the warm surface water layer. Measurements of variable fluorescence (F-v/F-m) indicated the greatest potential capacity for electron flow in photosystem II in phytoplankton was located below the warm surface waters. Spectrophotometrically determined pigment ratios suggest that the enhanced capacity for photosynthesis in the deeper waters may be related to greater nutrient availability here than in surface waters. Subsurface chlorophyll distributions seen in relation to the different water masses identified in the area, as well as community analysis of the phytoplankton present in the subsurface peaks, indicate the presence of at least three distinct subsurface phytoplankton blooms in the Skagerrak during the study period. Local oxygen saturation maxima recorded immediately above the subsurface peaks provide in situ evidence that these peaks are photosynthetically active. This suggests that new production is taking place in these peaks, although quantification of this production is hampered due to a lack of information concerning the initial conditions in and lifetime of the subsurface peaks. The subsurface phytoplankton peaks were, generally, found immediately above an oxygen minimum that covered the entire study area. In the relatively cold deep Atlantic water found below the oxygen minimum layer, no or very little chlorophyll was recorded and oxygen concentrations increased. Thus, it is argued that the respiration of the organic material produced in the upper part of the water column during late summer may primarily occur in the intermediate layers of the water column. [References: 35] LG - English PT - Article SB - Current Contents(R)/Agriculture, Biology & Environmental Sciences CC - Aquatic Sciences in Current Contents(R)/Agricultural, Biology & Environmental Sciences. EW - 2003 week 31 IN - Reprint available from: Richardson K Univ Aarhus, Dept Marine Ecol Finlandsgade 14 DK-8200 Aarhus Denmark Univ Aarhus, Dept Marine Ecol DK-8200 Aarhus Denmark Natl Environm Res Inst DK-4000 Roskilde Denmark <21> UI - 695BV-0002 DD - ISI Document Solution: 695BV AU - Akutagawa T AU - Uchimaru T AU - Sakai K AU - Hasegawa T AU - Nakamura T MA - takuta@imd.es.hokudai.ac.jp, tnaka@imd.es.hokudai.ac.jp RA - Akutagawa T TI - Bidentated hydrogen bond from [O-H center dot center dot center dot N-N center dot center dot center dot H-O] to [O-center dot center dot center dot H+-N-N-H+center dot center dot center dot O-] structures in solids SO - Journal of Physical Chemistry B. 107(26):6248-6251, 2003 Jul 3. AS - J. Phys. Chem. B 2003 Jul 3;107(26):6248-6251 PU - AMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 USA. URL: http://pubs.acs.org IS - 1520-6106 MH - Photosynthetic reaction-center MH - Proton-transfer MH - Molecular complexes MH - Charge-transfer MH - Rhodopseudomonas-viridis MH - Pentachlorophenol. AB - Heteronuclear bidentated N-H-O hydrogen bonds in the (4,4'-bipyridine)(polynitrophenols)(2) system were revealed in terms of X-ray structural analyses, ab-initio calculations, and vibrational spectra. 4,4'-Bipyridine (AA) and polynitrophenols (D) acted as Bronsted base and acid in solids, respectively. The bidentated hydrogenbonding interaction in solids was modified between [D-...+H-AA-H+...-D] and [D-H(...)AA(...)H-D] through regulating Bronsted acid-base conditions. [References: 27] LG - English PT - Letter SB - Current Contents(R)/Physical, Chemical & Earth Sciences CC - Physical Chemistry/Chemical Physics in Current Contents(R)/Physical, Chemical & Earth Sciences. EW - 2003 week 31 IN - Reprint available from: Akutagawa T Hokkaido Univ, Res Inst Elect Sci Sapporo Hokkaido 0600812 Japan Hokkaido Univ, Res Inst Elect Sci Sapporo Hokkaido 0600812 Japan JST, PRESTO Kawaguchi 3320012 Japan Natl Inst Adv Ind Sci & Technol Tsukuba Ibaraki 3058565 Japan <22> UI - 697BA-0002 DD - ISI Document Solution: 697BA AU - Dreuw A AU - Fleming GR AU - Head-Gordon M MA - andreas@bastille.cchem.berkeley.edu RA - Dreuw A TI - Charge-transfer state as a possible signature of a zeaxanthin-chlorophyll dimer in the non-photochemical quenching process in green plants SO - Journal of Physical Chemistry B. 107(27):6500-6503, 2003 Jul 10. AS - J. Phys. Chem. B 2003 Jul 10;107(27):6500-6503 PU - AMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 USA. URL: http://pubs.acs.org IS - 1520-6106 MH - Density-functional theory MH - Excess light energy MH - Xanthophyll cycle MH - Photosystem-ii MH - S-1 state MH - Fluorescence MH - Leaves MH - Approximation MH - Chloroplasts MH - Carotenoids. AB - Quantum chemical calculations of the electronic excited states of a zeaxanthin-chlorophyll dimer show that a zeaxanthin-to-chlorophyll charge-transfer (CT) state lies in the same energy regime as the excited states S-1 of zeaxanthin and Q(y) of chlorophyll, which are relevant for the nonphotochemical quenching (NPQ) process in green plants. Comparison with an analogous violaxanthin-chlorophyll dimer is made, and possible quenching mechanisms via excitation energy transfer or electron transfer are discussed. It is pointed out that the strongly allowed absorption of the zeaxanthin radical cation present in the CT state can be experimentally used as signature of the zea-chl dimer during the NPQ process. [References: 28] LG - English PT - Letter SB - Current Contents(R)/Physical, Chemical & Earth Sciences CC - Physical Chemistry/Chemical Physics in Current Contents(R)/Physical, Chemical & Earth Sciences. EW - 2003 week 31 IN - Reprint available from: Dreuw A Univ Calif Berkeley, Dept Chem Berkeley, CA 94720 USA Univ Calif Berkeley, Dept Chem Berkeley, CA 94720 USA Lawrence Berkeley Natl Lab, Div Chem Sci & Phys Biosci Berkeley, CA 94720 USA <23> UI - 697BA-0021 DD - ISI Document Solution: 697BA AU - Zheng XH AU - Stuchebrukhov AA RA - Stuchebrukhov AA TI - Electron tunneling in proteins: Implementation of ZINDO model for tunneling currents calculations SO - Journal of Physical Chemistry B. 107(27):6621-6628, 2003 Jul 10. AS - J. Phys. Chem. B 2003 Jul 10;107(27):6621-6628 PU - AMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 USA. URL: http://pubs.acs.org IS - 1520-6106 MH - Differential-overlap technique MH - Projected single determinant MH - Order density matrix MH - Intermediate neglect MH - Ab-initio MH - Rhodopseudomonas-viridis MH - Transfer kinetics MH - Redox pairs MH - Pathways MH - Donor. AB - The tunneling currents method developed previously by this group for the description of electron tunneling in proteins and other complex molecular structures is implemented at the semiempirical ZINDO level. This implementation bridges the gap between fully ab initio and simple one electron methods, such as the extended Huckel method, which has been previously employed in tunneling studies, and allows for accurate calculations to be performed on realistic large protein systems with only a moderate effort. One additional advantage of this method is that it employs the STO basis functions, which decay more slowly than do GTO functions, so that the weak tunneling interactions of noncovalently bonded atoms of the protein can be accounted for with a minimum basis set. Numerical results are demonstrated on the polypeptide chain (His)(2)(Met)Cu1+-(Cys)-(Gly(5))-(His)Ru-3 (+)(bpy(2))Im, which models the Ru-modified azurin charge transfer system studied by Gray and co-workers. [References: 59] LG - English PT - Article SB - Current Contents(R)/Physical, Chemical & Earth Sciences CC - Physical Chemistry/Chemical Physics in Current Contents(R)/Physical, Chemical & Earth Sciences. EW - 2003 week 31 IN - Reprint available from: Stuchebrukhov AA Univ Calif Davis, Dept Chem Davis, CA 95616 USA Univ Calif Davis, Dept Chem Davis, CA 95616 USA <24> UI - 697CC-0022 DD - ISI Document Solution: 697CC AU - Kulik LV AU - Borovykh IV AU - Gast P AU - Dzuba SA MA - chemphy@ns.kinetics.nsc.ru, dzuba@ns.kinetics.nsc.ru RA - Kulik LV TI - Selective excitation in pulsed EPR of a spin-correlated triplet-radical pair SO - Journal of Magnetic Resonance. 162(2):423-428, 2003 Jun. AS - J. Magn. Reson 2003 Jun;162(2):423-428 PU - ACADEMIC PRESS INC ELSEVIER SCIENCE, 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA. URL: http://www.apnet.com IS - 1090-7807 MH - Selective excitation MH - Out-of-phase fid MH - Eseem. MH - Photosynthetic reaction centers MH - Spectral diffusion MH - Photosystem-i MH - Spectroscopy MH - Relaxation MH - Exchange. AB - Experiments are described in which a low-amplitude microwave pulse excites only one out of three allowed transitions of the quinone radical (Q(A)(-)) in a spin-correlated triplet-radical pair (3)PQ(A)(-) of the bacterial photosynthetic reaction center. A second high-amplitude pulse produces a FID whose temporal shape is strongly modulated with frequencies determined by electron-electron dipolar interaction in the pair. The FID is detected in both the in-phase and the out-of-phase channels. The out-of-phase FID is a result of switching off the magnetic dipolar interaction between P-3 and Q(A)(-) due to decay of P-3 during the time interval between the two pulses. Refocusing of FID by an additional non-selective pulse allows a dead-time free measurement of this modulation. The influence of the dead-time problem on the distance determination is discussed. (C) 2003 Elsevier Science (USA). All rights reserved. [References: 16] LG - English PT - Article SB - Current Contents(R)/Life Sciences Current Contents(R)/Physical, Chemical & Earth Sciences CC - Chemistry & Analysis in Current Contents(R)/Life Sciences. Physical Chemistry/Chemical Physics in Current Contents(R)/Physical, Chemical & Earth Sciences. EW - 2003 week 31 IN - Reprint available from: Kulik LV Russian Acad Sci, Inst Chem Kinet & Combust Novosibirsk 630090 Russia Russian Acad Sci, Inst Chem Kinet & Combust Novosibirsk 630090 Russia Leiden Univ, Huygens Lab, Dept Biophys NL-2300 RA Leiden Netherlands <25> UI - 696YL-0005 DD - ISI Document Solution: 696YL AU - Park S AU - Sener MK AU - Lu DY AU - Schulten K MA - kshulte@ks.uiuc.edu RA - Park S TI - Reaction paths based on mean first-passage times SO - Journal of Chemical Physics. 119(3):1313-1319, 2003 Jul 15. AS - J. Chem. Phys 2003 Jul 15;119(3):1313-1319 PU - AMER INST PHYSICS, CIRCULATION & FULFILLMENT DIV, 2 HUNTINGTON QUADRANGLE, STE 1 N O 1, MELVILLE, NY 11747-4501 USA. URL: http://www.aip.org IS - 0021-9606 MH - Diffusion-controlled reactions MH - Cyanobacterial photosystem-i MH - Synechococcus-elongatus MH - Purple bacteria MH - Temperature. AB - Finding representative reaction pathways is important for understanding the mechanism of molecular processes. We propose a new approach for constructing reaction paths based on mean first-passage times. This approach incorporates information about all possible reaction events as well as the effect of temperature. As an application of this method, we study representative pathways of excitation migration in a photosynthetic light-harvesting complex, photosystem I. The paths thus computed provide a complete, yet distilled, representation of the kinetic flow of excitation toward the reaction center, thereby succinctly characterizing the function of the system. (C) 2003 American Institute of Physics. [References: 28] LG - English PT - Article SB - Current Contents(R)/Physical, Chemical & Earth Sciences CC - Physical Chemistry/Chemical Physics in Current Contents(R)/Physical, Chemical & Earth Sciences. EW - 2003 week 31 IN - Reprint available from: Park S Univ Illinois, Beckman Inst Urbana, IL 61801 USA Univ Illinois, Beckman Inst Urbana, IL 61801 USA Univ Illinois, Dept Phys Urbana, IL 61801 USA <26> UI - 696YL-0036 DD - ISI Document Solution: 696YL AU - Qian W AU - Jonas DM MA - david.jonas@colorado.edu RA - Qian W TI - Role of cyclic sets of transition dipoles in the pump-probe polarization anisotropy: Application to square symmetric molecules and perpendicular chromophore pairs SO - Journal of Chemical Physics. 119(3):1611-1622, 2003 Jul 15. AS - J. Chem. Phys 2003 Jul 15;119(3):1611-1622 PU - AMER INST PHYSICS, CIRCULATION & FULFILLMENT DIV, 2 HUNTINGTON QUADRANGLE, STE 1 N O 1, MELVILLE, NY 11747-4501 USA. URL: http://www.aip.org IS - 0021-9606 MH - Photosynthetic reaction-center MH - B850 antenna complex MH - Rhodobacter-sphaeroides MH - Room-temperature MH - Energy-transfer MH - Absorption anisotropy MH - 2-photon absorption MH - Quantum beats MH - Fluorescence MH - Dynamics. AB - Theoretical models considering inhomogeneous energy splitting effects are developed to explain impulsive pump-probe polarization anisotropy experiments on degenerate states of square symmetric molecules and chromophore pairs with perpendicular transition dipoles. When detecting the pump-induced change in probe transmission through the sample, the pump-probe signal arises from lost absorption due to ground state depopulation, stimulated emission from the singly excited states, and absorption from the singly excited states to doubly excited states. Including coherence between the singly excited states, the total impulsive initial anisotropy is 2/5 in all cases. In contrast to the first and second contributions, the time dependent excited state absorption anisotropy depends on a cyclic set of four transition dipoles, not just the energetic splitting. Ignoring molecular rotation, inhomogeneous anisotropy dynamics arise only for coupled chromophore pairs and Jahn-Teller distortions of the degenerate state that produce a cyclic set of transition dipoles different from the set for uncoupled direct product states. In all systems treated, a time dependent pump-probe anisotropy requires coupling between excitations. (C) 2003 American Institute of Physics. [References: 44] LG - English PT - Article SB - Current Contents(R)/Physical, Chemical & Earth Sciences CC - Physical Chemistry/Chemical Physics in Current Contents(R)/Physical, Chemical & Earth Sciences. EW - 2003 week 31 IN - Reprint available from: Qian W Univ Colorado, Dept Chem & Biochem Campus Box 215 Boulder, CO 80309 USA Univ Colorado, Dept Chem & Biochem Boulder, CO 80309 USA <27> UI - 696QJ-0015 DD - ISI Document Solution: 696QJ AU - Yee PL AU - Hassan MA AU - Shirai Y AU - Wakisaka M AU - Karim MIA MA - shirai@life.kyutech.ac.jp RA - Shirai Y TI - Continuous production of organic acids from palm oil mill effluent with sludge recycle by the freezing-thawing method SO - Journal of Chemical Engineering of Japan. 36(6):707-710, 2003 Jun. AS - J. Chem. Eng. Jpn 2003 Jun;36(6):707-710 PU - SOC CHEMICAL ENG JAPAN, KYORITSU BUILDING 4-16-19 KOHINATA, BUNKYO KU TOKYO, 112, JAPAN IS - 0021-9592 MH - Organic acids MH - Palm oil mill effluent MH - Anaerobic treatment MH - Recycle MH - Freezing-thawing. MH - Rhodobacter-sphaeroides MH - Anaerobic treatment MH - Polyhydroxyalkanoates. AB - The performance of the anaerobic treatment of palm oil mill effluent for organic acids production at a short retention time of less than 5 days was assessed by incorporating a sludge recycle system with no pH control except by adding calcium carbonate. The system could be operated successfully for a 3.5-day retention time at pH 5. The sludge solids in the treated effluent were separated by a freezing-thawing technique and it was partly recycled back to the reactor. The thawed clarified POME solution contained a low SS of 2,200-3,500 mg/L. The organic acids production with a concentration of 10-14 g/L was essentially similar to the treatment for a 5-day retention time without a sludge recycle. Hence by incorporating a sludge recycle system with the freezing-thawing method, the retention time for the treatment could be reduced without affecting the organic acids generation. [References: 11] LG - English PT - Article SB - Current Contents(R)/Engineering, Computing & Technology CC - Chemical Engineering in Current Contents(R)/Engineering, Computing & Technology. EW - 2003 week 31 IN - Reprint available from: Shirai Y Kyushu Inst Technol, Fac Comp Sci & Syst Engn, Dept Biochem Engn & Sci Fukuoka 8208502 Japan Kyushu Inst Technol, Fac Comp Sci & Syst Engn, Dept Biochem Engn & Sci Fukuoka 8208502 Japan Univ Putra Malaysia, Fac Food Sci & Biotechnol, Dept Biotechnol Serdang 43400 Selangor Malaysia Kyushu Inst Technol, Grad Sch Life Sci & Syst Engn Kitakyushu Fukuoka 8080196 Japan <28> UI - 697AN-0064 DD - ISI Document Solution: 697AN AU - Gin P AU - Hsu AY AU - Rothman SC AU - Jonassen T AU - Lee PT AU - Tzagoloff A AU - Clarke CF MA - cathy@mbi.ucla.edu RA - Clarke CF TI - The Saccharomyces cerevisiae COQ6 gene encodes a mitochondrial flavin-dependent monooxygenase required for coenzyme Q biosynthesis SO - Journal of Biological Chemistry. 278(28):25308-25316, 2003 Jul 11. AS - J. Biol. Chem 2003 Jul 11;278(28):25308-25316 PU - AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA. URL: http://www.asbmb.org IS - 0021-9258 MH - Escherichia-coli k-12 MH - Amino-acid-sequence MH - Ubiquinone biosynthesis MH - Hydroxybenzoate hydroxylase MH - Caenorhabditis-elegans MH - Salicylate hydroxylase MH - Functional expression MH - Intermembrane space MH - Pseudomonas-putida MH - Yeast mitochondria. AB - Coenzyme Q (Q) is a lipid that functions as an electron carrier in the mitochondrial respiratory chain in eukaryotes. There are eight complementation groups of Q-deficient Saccharomyces cerevisiae mutants, designated coq1-coq8. Here we have isolated the COQ6 gene by functional complementation and, in contrast to a previous report, find it is not an essential gene. coq6 mutants are unable to grow on nonfermentable carbon sources and do not synthesize Q but instead accumulate the Q biosynthetic intermediate 3-hexaprenyl-4-hydroxybenzoic acid. The Coq6 polypeptide is imported into the mitochondria in a membrane potential-dependent manner. Coq6p is a peripheral membrane protein that localizes to the matrix side of the inner mitochondrial membrane. Based on sequence homology to known proteins, we suggest that COQ6 encodes a flavin-dependent monooxygenase required for one or more steps in Q biosynthesis. [References: 65] LG - English PT - Article SB - Current Contents(R)/Life Sciences CC - Biochemistry & Biophysics in Current Contents(R)/Life Sciences. EW - 2003 week 31 IN - Reprint available from: Clarke CF Univ Calif Los Angeles, Dept Biochem & Chem 607 Charles E Young Dr E Los Angeles, CA 90095 USA Univ Calif Los Angeles, Dept Biochem & Chem Los Angeles, CA 90095 USA Univ Calif Los Angeles, Inst Mol Biol Los Angeles, CA 90095 USA Columbia Univ, Dept Biol Sci New York, NY 10027 USA <29> UI - 697AN-0068 DD - ISI Document Solution: 697AN AU - Gong X AU - Xie T AU - Yu L AU - Hesterberg M AU - Scheide D AU - Friedrich T AU - Yu CA MA - cayuq@okstate.edu RA - Yu CA TI - The ubiquinone-binding site in NADH : ubiquinone oxidoreductase from Escherichia coli SO - Journal of Biological Chemistry. 278(28):25731-25737, 2003 Jul 11. AS - J. Biol. Chem 2003 Jul 11;278(28):25731-25737 PU - AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA. URL: http://www.asbmb.org IS - 0021-9258 MH - Proton-translocating nadh MH - Mitochondrial complex-i MH - Hereditary optic neuropathy MH - Respiratory-chain MH - Quinone-binding MH - Subunit MH - Reductase MH - Membrane MH - Identification MH - Domain. AB - An azido-ubiquinone derivative, 3-azido-2-methyl-5-methoxy[ H-3]-6-decyl-1,4-benzoquinone ([H-3] azido-Q), was used to study the ubiquinone/protein interaction and to identify the ubiquinone-binding site in Escherichia coli NADH: ubiquinone oxidoreductase ( complex I). The purified complex I showed no loss of activity after incubation with a 20-fold molar excess of [H-3] azido-Q in the dark. Illumination of the incubated sample with long wavelength UV light for 10 min at 0 degreesC caused a 40% decrease of NADH: ubiquinone oxidoreductase activity. SDS-PAGE of the complex labeled with [H-3] azido-Q followed by analysis of the radioactivity distribution among the subunits revealed that subunit NuoM was heavily labeled, suggesting that this protein houses the Q-binding site. When the [H-3] azido-Q-labeled NuoM was purified from the labeled reductase by means of preparative SDS-PAGE, a 3-azido-2-methyl-5-methoxy-6-decyl-1,4- benzoquinone-linked peptide, with a retention time of 41.4 min, was obtained by high performance liquid chromatography of the protease K digest of the labeled subunit. This peptide had a partial NH2-terminal amino acid sequence of NH2-VMLIAILALV-, which corresponds to amino acid residues 184 - 193 of NuoM. The secondary structure prediction of NuoM using the Toppred hydropathy analysis showed that the Q-binding peptide overlaps with a proposed Q-binding motif located in the middle of the transmembrane helix 5 toward the cytoplasmic side of the membrane. Using the PHDhtm hydropathy plot, the labeled peptide is located in the transmembrane helix 4 toward the periplasmic side of the membrane. [References: 43] LG - English PT - Article SB - Current Contents(R)/Life Sciences CC - Biochemistry & Biophysics in Current Contents(R)/Life Sciences. EW - 2003 week 31 IN - Reprint available from: Yu CA Oklahoma State Univ, Dept Biochem & Mol Biol, Noble Res Ctr 255 Stillwater, OK 74078 USA Oklahoma State Univ, Dept Biochem & Mol Biol, Noble Res Ctr 255 Stillwater, OK 74078 USA Univ Freiburg, Inst Organ Chem & Biochem D-79104 Freiburg Germany <30> UI - 697GU-0027 DD - ISI Document Solution: 697GU AU - Gacouin A AU - Lavoue S AU - Signouret T AU - Person A AU - Dinard MD AU - Shpak N AU - Thomas R MA - arnaud.gacouin@chu-rennes.fr RA - Gacouin A TI - Reversible spongiform leucoencephalopathy after inhalation of heated heroin SO - Intensive Care Medicine. 29(6):1012-1015, 2003 Jun. AS - Intensive Care Med 2003 Jun;29(6):1012-1015 PU - SPRINGER-VERLAG, 175 FIFTH AVE, NEW YORK, NY 10010 USA. URL: http://www.springer-ny.com IS - 0342-4642 MH - Coma MH - Toxic leucoencephalopathy MH - Spongiform leucoencephalopathy MH - Heroin intoxication. MH - Toxic leukoencephalopathy MH - Inhaling heroin. AB - Objective: To describe the clinical course and imaging findings in a young man who developed a spongiform leticoencephalopathy from heroin-vapour inhalation, and to discuss the treatments which may have contributed to the unexpected favourable outcome in this case. Design: Case report. Setting: Intensive care unit of a university teaching hospital. Patient: A patient who developed a near fatal toxic leucoencephalopathy with impressive clinical recovery and reversible white matter changes on imaging. Measurements and results: Successive computed tomography scans and magnetic resonance imaging over 7 months showed evolution from bilateral extensive involvement of the cerebral white matter to almost complete resolution accompanied by the development of periventricular lesions suggestive of necrosis. Despite the fact that the patient had stretching spasms for several days, the outcome was favourable with prolonged supportive care and antioxidant therapy by ubiquinone (coenzyme Q). Conclusion: This case demonstrates that prolonged intensive care is of paramount importance in patients with spongiform leucoencephalopathy after inhalation of heated heroin, that abnormalities of cerebral white matter may be slowly regressive, and supports the use of coenzyme Q in severe forms of the disease. [References: 7] LG - English PT - Article SB - Current Contents(R)/Clinical Medicine CC - Anesthesia & Intensive Care in Current Contents(R)/Clinical Medicine. EW - 2003 week 31 IN - Reprint available from: Gacouin A CHU Rennes, Hop Pontchaillou, Serv Malad Infect & Reanimat Med Rue Henri Le Guilloux F-35033 Rennes France CHU Rennes, Hop Pontchaillou, Serv Malad Infect & Reanimat Med F-35033 Rennes France CHU Rennes, Serv Pharmacol Rennes France CHRU Rennes, Serv Med Phys & Readaptat Rennes France <31> UI - 697WG-0002 DD - ISI Document Solution: 697WG AU - Olsen RKJ AU - Andresen BS AU - Christensen E AU - Bross P AU - Skovby F AU - Gregersen N MA - rikke.olsen@mmf.au.dk RA - Olsen RKJ TI - Clear relationship between ETF/ETFDH genotype and phenotype in patients with multiple Acyl-CoA dehydrogenation deficiency SO - Human Mutation. 22(1):12-23, 2003 Jul. AS - Hum. Mutat 2003 Jul;22(1):12-23 PU - WILEY-LISS, DIV JOHN WILEY & SONS INC, 605 THIRD AVE, NEW YORK, NY 10158-0012 USA. URL: http://www.wiley.com IS - 1059-7794 MH - Multiple acyl-coa dehydrogenation deficiency MH - Madd MH - Glutaric aciduria type ii MH - Genotype-phenotype MH - Mutation analysis. MH - Electron-transfer flavoprotein MH - Acidemia type-ii MH - Aciduria type-ii MH - Congenital polycystic kidneys MH - Symmetric warty dysplasia MH - Ubiquinone oxidoreductase MH - Beta-oxidation MH - Alpha-subunit MH - Cultured fibroblasts MH - Molecular-cloning. AB - Mutations in electron transfer flavoprotein (ETF) and its dehydrogenase (ETFDH) are the molecular basis of multiple acyl-CoA dehydrogenation deficiency (MADD), an autosomal recessively inherited and clinically heterogeneous disease that has been divided into three clinical forms: a neonatal-onset form with congenital anomalies (type I), a neonatal-onset form without congenital anomalies (type II), and a late,onset form (type III). To examine whether these different clinical forms could be explained by different ETF/ETFDH mutations that result in different levels of residual ETF/ETTDH enzyme activity, we have investigated the molecular genetic basis for disease development in nine patients representing the phenotypic spectrum of MADD. We report the genomic structures of the ETFA, ETFB, and ETFDH genes and the identification and characterization of seven novel and three previously reported disease,causing mutations. Our molecular genetic investigations of these nine patients are consistent with three clinical forms of MADD showing a clear relationship between the nature of the mutations and the severity of disease. Interestingly, our data suggest that homozygosity for two null mutations causes fetal development of congenital anomalies resulting in a type I disease phenotype. Even minute amounts of residual ETF/ETFDH activity seem to be sufficient to prevent embryonic development of congenital anomalies giving rise to type II disease. Overexpression studies of an ETFB-D128N missense mutation identified in a patient with type III disease showed that the residual activity of the mutant enzyme could be rescued up to 59% of that of wild-type activity when ETFB-D128N-transformed E. coli cells were grown at low temperature. This indicates that the effect of the ETF/ETFDH genotype in patients with milder forms of MADD, in whom residual enzyme activity allows modulation of the enzymatic phenotype, may be influenced by environmental factors like cellular temperature. (C) 2003 Wiley-Liss, Inc. [References: 40] LG - English PT - Article SB - Current Contents(R)/Life Sciences CC - Molecular Biology & Genetics in Current Contents(R)/Life Sciences. EW - 2003 week 31 IN - Reprint available from: Olsen RKJ Skejby Sygehus, Res Unit Mol Med, Fac Hlth Sci Brendstrup Gaardsvej 100 DK-8200 Aarhus N Denmark Skejby Sygehus, Res Unit Mol Med, Fac Hlth Sci DK-8200 Aarhus N Denmark Univ Aarhus, Res Unit Mol Med Aarhus Denmark Univ Aarhus, Inst Human Genet Aarhus Denmark Rigshosp, Dept Clin Genet DK-2100 Copenhagen Denmark <32> UI - 694ZC-0006 DD - ISI Document Solution: 694ZC AU - Hendrickson L AU - Ball MC AU - Osmond CB AU - Furbank RT AU - Chow WS MA - hendrickson@rsbs.anu.edu.au RA - Hendrickson L TI - Assessment of photoprotection mechanisms of grapevines at low temperature SO - Functional Plant Biology. 30(6):631-642, 2003. AS - Funct. Plant Biol 2003;30(6):631-642 PU - C S I R O PUBLISHING, 150 OXFORD ST, PO BOX 1139, COLLINGWOOD, VICTORIA 3066, AUSTRALIA. URL: http://www.publish.csiro.au IS - 1445-4408 MH - Mehler reaction MH - Non-photochemical quenching MH - Photoinactivation MH - Photoprotection MH - Photorespiration MH - Photosynthesis MH - Temperature MH - Vitis vinifera. MH - Photosynthetic electron-transport MH - Functional photosystem-ii MH - Xanthophyll cycle MH - Mehler reaction MH - Chlorophyll fluorescence MH - Co2 assimilation MH - Quantum yield MH - In-vivo MH - Chilling temperatures MH - Energy-dissipation. AB - The photosynthetic response of grapevine leaves (Vitis vinifera L. cv. Riesling) to low temperature was studied in the field and laboratory. Light-saturated rates of photosynthetic electron transport were lower and non-photochemical energy dissipation was higher when leaves were subject to low morning temperatures than to high afternoon temperatures under field conditions. These responses to low temperatures occurred without sustained reduction of quantum efficiency of PSII as measured by the variable to maximum chlorophyll fluorescence yield ratio, F-v/F-m, after dark adaptation. The temperature dependence of light-saturated apparent electron transport rate, gas exchange and non-photochemical quenching (NPQ) was also examined in laboratory experiments with glasshouse-grown material. NPQ reached saturation at lower light intensity with decreasing temperature. The relationship between the quantum efficiency of PSII and CO2 fixation at 25degreesC (2 - 21% O-2) and 10 degreesC ( 2 - 21% O-2) indicated a decreased dependence of electron transport on both photorespiration and the Mehler reaction at the lower temperature. The calculated percentage of electron flow to the Mehler reaction declined faster than photorespiration at low temperature. Warm- and cold-treated leaf discs under saturating light showed very little photoinhibition as measured by sustained reduction in F-v/F-m, which was linearly related to the percentage of functional PSII reaction centres. However, the addition of dithiothreitol greatly enhanced the rate of photoinhibition, indicating a potentially strong dependence on xanthophyll de-epoxidation for photoprotection at low temperature. [References: 60] LG - English PT - Article SB - Current Contents(R)/Agriculture, Biology & Environmental Sciences Current Contents(R)/Life Sciences CC - Plant Sciences in Current Contents(R)/Agricultural, Biology & Environmental Sciences. Animal & Plant Sciences in Current Contents(R)/Life Sciences. EW - 2003 week 31 IN - Reprint available from: Hendrickson L Australian Natl Univ, Res Sch Biol Sci GPO Box 475 Canberra ACT 2601 Australia Australian Natl Univ, Res Sch Biol Sci Canberra ACT 2601 Australia Columbia Univ, Biosphere Ctr 2 Oracle, AZ 85623 USA CSIRO Plant Ind Canberra ACT 2601 Australia <33> UI - 694ZC-0008 DD - ISI Document Solution: 694ZC AU - de Souza CR AU - Maroco JP AU - dos Santos TP AU - Rodrigues ML AU - Lopes CM AU - Pereira JS AU - Chaves MM MA - mchaves@isa.utl.pt RA - Chaves MM TI - Partial rootzone drying: regulation of stomatal aperture and carbon assimilation in field-grown grapevines (Vitis vinifera cv. Moscatel) SO - Functional Plant Biology. 30(6):653-662, 2003. AS - Funct. Plant Biol 2003;30(6):653-662 PU - C S I R O PUBLISHING, 150 OXFORD ST, PO BOX 1139, COLLINGWOOD, VICTORIA 3066, AUSTRALIA. URL: http://www.publish.csiro.au IS - 1445-4408 MH - Carbon isotope composition MH - Fluorescence MH - Gas exchange MH - Partial rootzone drying MH - Vitis vinifera MH - Water stress. MH - Electron-transport rate MH - Water-stress MH - Chlorophyll fluorescence MH - Transpiration efficiency MH - Isotope discrimination MH - Co2 assimilation MH - Photosystem-ii MH - Abscisic-acid MH - Gas-exchange MH - Plants. AB - The effects of 'partial rootzone drying' (PRD) irrigation compared with other irrigation systems, namely non-irrigated NI), full irrigation (FI) and deficit irrigation (DI), on stomatal conductance and carbon assimilation were evaluated in field-grown grapevines ( Vitis vinifera L. cv. Moscatel). At the end of the growing season, pre-dawn leaf water potential was highest in FI (-0.18 +/- 0.01 MPa; mean +/- s.e.), intermediate in PRD (-0.30 +/- 0.01 MPa) and DI ( -0.36 +/- 0.02 MPa), and lowest in NI vines ( -0.64 +/- 0.03 MPa). Stomatal conductance measured under controlled conditions of light and temperature was reduced in NI (ca 60%) and PRD ( ca 30%) vines compared with DI and FI vines. Under ambient conditions, NI vines had lower rates of stomatal conductance (ca 26%), net CO2 assimilation (ca 28%) and light-adapted PSII quantum yields (ca 47%) than PRD, DI and FI vines. No significant differences were found among the three irrigated treatments. Both maximum electron transport rate (J(max); ca 30%) and triose-phosphate utilization rates (TPU; ca 20%) were significantly lower in NI and PRD vines than in DI and FI vines. Carbon isotope composition (delta(13)C) of grape berries was highest in NI vines ( -24.3parts per thousand), followed by PRD ( - 25.4parts per thousand) and DI ( - 25.8parts per thousand) and lowest in FI ( - 26.4parts per thousand) vines, suggesting a long-term increase in the efficiency of leaf gas exchange in NI compared with PRD, DI and FI vines. Sap-flow data and estimates of relative stomatal limitation are in accordance with the observed stomatal closure in PRD vines. In this study, we show that PRD irrigation was able to maintain a vine water status closed to FI, but with double water use efficiency, which was due to a reduction of stomatal conductance with no significant decrease in carbon assimilation. [References: 51] LG - English PT - Article SB - Current Contents(R)/Agriculture, Biology & Environmental Sciences Current Contents(R)/Life Sciences CC - Plant Sciences in Current Contents(R)/Agricultural, Biology & Environmental Sciences. Animal & Plant Sciences in Current Contents(R)/Life Sciences. EW - 2003 week 31 IN - Reprint available from: Chaves MM Inst Tecnol Quim & Biol, Lab Ecofisiol Mol Apartado 127 P-2780901 Oeiras Portugal Inst Tecnol Quim & Biol, Lab Ecofisiol Mol P-2780901 Oeiras Portugal Inst Super Psicol Aplicada P-1149047 Lisbon Portugal Inst Super Agron P-1349017 Lisbon Portugal <34> UI - 694KU-0003 DD - ISI Document Solution: 694KU AU - Nilsson SEG AU - Sundelin SP AU - Wihlmark U AU - Brunk UT MA - svenerik.nilsson@eye.liu.se RA - Nilsson SEG TI - Aging of cultured retinal pigment epithelial cells: oxidative reactions, lipofuscin formation and blue light damage SO - Documenta Ophthalmologica. 106(1):13-16, 2003 Jan. AS - Doc. Ophthalmol 2003 Jan;106(1):13-16 PU - KLUWER ACADEMIC PUBL, VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS. URL: http://www.wkap.nl IS - 0012-4486 MH - Antioxidants MH - Blue light damage MH - Lipofuscin MH - Melanin MH - Oxidative reactions MH - Retinal pigment epithelium. MH - Outer segments MH - Macular degeneration MH - Accumulation MH - Ultrastructure MH - Membranes MH - Eye. AB - This report reviews our experimental work on cultured retinal pigment epithelial (RPE) cells, fed native or UV-irradiated photoreceptor outer segments (POS). We showed that significantly more lipofuscin (LF) was formed in cells cultured in 40% oxygen than in cells cultured in 8% oxygen, indicating an involvement of oxidative mechanisms in LF formation. The antioxidants alpha-tocopherol, lycopene, zeaxanthin and lutein significantly reduced LF formation. RPE cells high in melanin content exhibited significantly less formation of LF than cells low in or devoid of melanin, suggesting that melanin acts as an effective antioxidant. The phagocytic capacity of LF-loaded RPE cells was significantly reduced compared to that of unloaded control cells, indicating that LF-loaded RPE cells may be unable to serve the photoreceptors sufficiently regarding phagocytosis of shed outer segment tips. Blue light irradiation destabilized lysosomal membranes in LF-loaded RPE cells and significantly reduced the viability of such cells compared to unloaded, irradiated control cells. These results may be of significance in relation to the development of age-related macular degeneration (AMD). [References: 26] LG - English PT - Article SB - Current Contents(R)/Clinical Medicine CC - Ophthalmology in Current Contents(R)/Clinical Medicine. EW - 2003 week 31 IN - Reprint available from: Nilsson SEG Linkoping Univ, Dept Ophthalmol SE-58185 Linkoping Sweden Linkoping Univ, Dept Ophthalmol SE-58185 Linkoping Sweden Linkoping Univ, Dept Pathol 2 SE-58185 Linkoping Sweden <35> UI - 696CG-0007 DD - ISI Document Solution: 696CG AU - Vaillancourt RD AU - Sambrotto RN AU - Green S AU - Matsuda A MA - vaillanc@ldeo.columbia.edu RA - Vaillancourt RD TI - Phytoplankton biomass and photosynthetic competency in the summertime Mertz Glacier Region of East Antarctica SO - Deep-Sea Research Part II-Topical Studies in Oceanography. 50(8-9):1415-1440, 2003. AS - Deep-Sea Res. Part II-Top. Stud. Oceanogr 2003;50(8-9):1415-1440 PU - PERGAMON-ELSEVIER SCIENCE LTD, THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND. URL: http://www.elsevier.nl IS - 0967-0645 MH - Southern-ocean MH - Sea-ice MH - Ross sea MH - Austral summer MH - Primary productivity MH - Bransfield strait MH - Continental-shelf MH - Katabatic winds MH - March 1996 MH - Waters. AB - Vertical profiles of water temperature, salinity, beam transmission, density, pressure, wind speed, wind direction, phytoplankton biomass (chlorophyll a (Chl a) plus phaeophytin a), and photosynthetic competency (by fast repetition-rate fluorometry) are presented for the Mertz Glacier region, East Antarctica, for a 3-week period during the austral summer 2000-2001. Injection of low-salinity water from the melting of the ice pack formed a shallow (ca. 25 m) mixed layer offshore. Two distinct deep mixing features were observed associated to varying degrees with high chlorophyll levels and high photosynthetic competency. Along the Adelie Land coast,. a deep mixing layer (1400 m) with Chl a concentrations of 386mg m(-2) and elevated F-v/F-m (>0.5) was observed. At the eastern end of the study area, along a seaward extension of fast-ice, a bloom of Phaeocystis antarctica bloom formed within a shallow (24 m) mixed layer, with surface Chl a concentrations of ca. 8 mg m(-3) and elevated Fv/Fm (0.5). This feature also had high surface salinity (> 34) and was contiguous with a deep-mixing feature (236 m). FRRF parameters F-v/F-m and sigma(PSII) were strongly suppressed in surface waters (< 20 m). Results of photoinhibition experiments showed that the photo-suppression of Fv/Fm and ups,, in surface waters was reversible over a time period of minutes to hours and suggested that the surface suppression was due to non-photochemical quenching by photo-protective pigments and photo-damage to Photosytem II reaction centers and pigment beds. FRRF data at a depth of 45-55 m were independent of irradiance, and provided a reasonable index of the nutrient status of the phytoplankton. The spatial coherence of plant biomass, photo-competency, high salinity and deep-mixing suggested that blooms of Phaeocystis and diatoms form in this region after physical disturbances result in mixing of nutrient-rich subsurface waters into the euphotic zone. The chlorophyll fluorescence and beam transmissometer profiles at the coastal diatom bloom indicated that much of this biomass was transported to shelf sediments by sinking or a downward advection and/or mixing mechanism. Possible physical mechanisms to account for this process are discussed, such as downward advection from sea-ice and brine formation, katabatic wind-induced mixing, and upwelling/downwelling, but insufficient data preclude the identification of the definitive causative mechanism. It is hypothesized that the coastal region in Adelie Land is a site of intense and frequent diatom blooms that transport large amounts of organic matter to depth. The presence of large accumulations of silicious ooze below the Mertz Polynya in the Adelie Depression suggests that this 'biological pumping' mechanism has been functioning for most of the Holocene. (C) 2003 Elsevier Science Ltd. All rights reserved. [References: 54] LG - English PT - Article SB - Current Contents(R)/Agriculture, Biology & Environmental Sciences Current Contents(R)/Physical, Chemical & Earth Sciences CC - Aquatic Sciences in Current Contents(R)/Agricultural, Biology & Environmental Sciences. Earth Sciences in Current Contents(R)/Physical, Chemical & Earth Sciences. EW - 2003 week 31 IN - Reprint available from: Vaillancourt RD Lamont Doherty Earth Observ Marine Biol Bldg,61 Route 9W Palisades, NY 10964 USA Lamont Doherty Earth Observ Palisades, NY 10964 USA <36> UI - 696UZ-0016 DD - ISI Document Solution: 696UZ AU - Basu P AU - Stolz JF AU - Smith MT MA - basu@duq.edu RA - Basu P TI - A coordination chemist's view of the active sites of mononuclear molybdenum enzymes [Review] SO - Current Science. 84(11):1412-1418, 2003 Jun 10. AS - Curr. Sci 2003 Jun 10;84(11):1412-1418 PU - CURRENT SCIENCE ASSN, C V RAMAN AVENUE, PO BOX 8005, BANGALORE 560 080, INDIA IS - 0011-3891 MH - Dimethyl-sulfoxide reductase MH - Crystal-structure MH - Sulfite oxidase MH - Dmso reductase MH - Xanthine dehydrogenase MH - Rhodobacter-capsulatus MH - Resonance raman MH - High-resolution MH - Charge-transfer MH - Complexes. AB - A comparison of the coordination environment of twelve structurally characterized mononuclear molybdenum-containing enzymes revealed many similarities that helped, define specific features of the active sites. Using bond distances and angles, the types of bonds and terminal groups of significance were identified. For example, a distance between molybdenum and oxygen atoms ranging from 1.6 to 1.8 Angstrom was considered as a Mo=O bond. The dithiolene sulphur to molybdenum single bond distances fluctuated around 2.40 Angstrom. In most cases, the sulphur-sulphur interaction within the co-factors is weak, with distances above 3.00 Angstrom, but both trimethylamine N-oxide and dissimilatory nitrate reductase (i.e. NapA) show strong sulphur-sulphur interactions with distances closer to 2.80 Angstrom. In addition, the dithiolene bite angle (S-Mo-S) was observed to be directly affected by the distance of the sulphurs from the metal centre. The bite angle is very small for co-factors that are further away from the molybdenum atom, as observed in TMAOR. A small bite angle is suggestive of ligand dissociation. [References: 49] LG - English PT - Review SB - Current Contents(R)/Agriculture, Biology & Environmental Sciences Current Contents(R)/Physical, Chemical & Earth Sciences CC - Multidisciplinary in Current Contents(R)/Agricultural, Biology & Environmental Sciences. Multidisciplinary in Current Contents(R)/Physical, Chemical & Earth Sciences. EW - 2003 week 31 IN - Reprint available from: Basu P Duquesne Univ, Dept Chem & Biochem Pittsburgh, PA 15282 USA Duquesne Univ, Dept Chem & Biochem Pittsburgh, PA 15282 USA Duquesne Univ, Dept Sci Biol Pittsburgh, PA 15282 USA <37> UI - 696UZ-0017 DD - ISI Document Solution: 696UZ AU - Bhattacharjee SK MA - swapan1943@yahoo.co.in RA - Bhattacharjee SK TI - Thermoluminescence from spinach leaf without excitation by any radiation or external stimuli: Stimulatory role of thermal fluctuations SO - Current Science. 84(11):1419-1427, 2003 Jun 10. AS - Curr. Sci 2003 Jun 10;84(11):1419-1427 PU - CURRENT SCIENCE ASSN, C V RAMAN AVENUE, PO BOX 8005, BANGALORE 560 080, INDIA IS - 0011-3891 MH - Containing plant material MH - Delayed light-emission MH - Photosystem-ii MH - Thin-films MH - Luminescence MH - Chloroplasts MH - Photosynthesis MH - Energetics MH - Particles MH - Field. AB - This article argues that the sources of glow seen by the thermoluminescence (TL) technique may be from largely in vivo biological nanoparticles with property of quantum confinement that entails trapping of energy and delayed emission, not solely due to the charges that undergo recombination. TL in spinach leaf, following prolonged period of idling in dark at ambient temperatures but without any excitation, has been observed. Occasional loss of stringent control of heating rate revealed that fluctuations generated complex TL structures. Following the trails of this observation we discovered that ripples larger than +/- 1degreesC gave rise to multiple small bands and spikes which were missing when the rate was controlled within +/- 1degreesC, both with or without excitation by light. The results do not warrant the application of Randall and Wilkins' theory to interpret TL from photosynthetic materials. [References: 31] LG - English PT - Article SB - Current Contents(R)/Agriculture, Biology & Environmental Sciences Current Contents(R)/Physical, Chemical & Earth Sciences CC - Multidisciplinary in Current Contents(R)/Agricultural, Biology & Environmental Sciences. Multidisciplinary in Current Contents(R)/Physical, Chemical & Earth Sciences. EW - 2003 week 31 IN - Reprint available from: Bhattacharjee SK Devi Ahila Vishwavidyalaya, Sch Life Sci Kandwa Rd Indore 452017 Madhya Pradesh India Devi Ahila Vishwavidyalaya, Sch Life Sci Indore 452017 Madhya Pradesh India Bhabha Atom Res Ctr, Mol Biol & Agr Div Bombay 400085 Maharashtra India <38> UI - 697WL-0019 DD - ISI Document Solution: 697WL AU - Ivashin N AU - Larsson S MA - larsson@phc.chalmers.se RA - Larsson S TI - Electron transfer pathways in photosystem I reaction centers SO - Chemical Physics Letters. 375(3-4):383-387, 2003 Jul 3. AS - Chem. Phys. Lett 2003 Jul 3;375(3-4):383-387 PU - ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS. URL: http://www.elsevier.nl IS - 0009-2614 MH - Photosynthetic reaction centers MH - Charge separation MH - Chlamydomonas-reinhardtii MH - Temperature MH - Vitamin-k1 MH - Spectroscopy MH - Molecules MH - Acceptor MH - Branches MH - Psaa. AB - Electron transfer following charge separation in the photosystem I (PSI) reaction center of Synechococcus elongatus is studied using theoretical methods. The difference in rate between two almost symmetrical A- and B-branches is caused by a difference in a single residue (Trp B673 versus Gly A693), close to the F-X iron-sulfur cluster. Partly due to its polar environment, Trp B673 acts as an electron acceptor in its pi-system. The rate increases on the B-side due to shortened distances for electron transfer. (C) 2003 Elsevier Science B.V. All rights reserved. [References: 33] LG - English PT - Article SB - Current Contents(R)/Physical, Chemical & Earth Sciences CC - Physical Chemistry/Chemical Physics in Current Contents(R)/Physical, Chemical & Earth Sciences. EW - 2003 week 31 IN - Reprint available from: Larsson S Chalmers Univ Technol, Dept Phys Chem S-41296 Gothenburg Sweden Chalmers Univ Technol, Dept Phys Chem S-41296 Gothenburg Sweden Natl Acad Sci, Inst Atom & Mol Phys Minsk 220072 Byelarus <39> UI - 696BM-0003 DD - ISI Document Solution: 696BM AU - Casazza G AU - Mazzella L MA - casazza@apat.it RA - Casazza G TI - Photosynthetic pigment composition of marine angiosperms: Preliminary characterization of Mediterranean seagrasses SO - Bulletin of Marine Science. 71(3):1171-1181, 2002 Nov. AS - Bull. Mar. Sci 2002 Nov;71(3):1171-1181 PU - ROSENSTIEL SCH MAR ATMOS SCI, 4600 RICKENBACKER CAUSEWAY, MIAMI, FL 33149 USA. URL: http://www.rsmas.miami.edu/bms/ IS - 0007-4977 MH - Light-intensity MH - Chromatic adaptation MH - L eelgrass MH - Depth MH - Ultraviolet MH - Radiation MH - Algae MH - Photoadaptation MH - Siphonaxanthin MH - Chromatography. AB - Pigments were investigated in the Mediterranean marine angiosperms Posidonia oceanica, Cymodocea nodosa, Zostera noltii and Halophila stipulacea. Samples were collected and analyzed from shallow and deep water habitats. Acetone extracts of leaves were analyzed by absorption spectroscopy, high performance liquid chromatography (HPLC) and thin layer chromatography (TLC). The marine angiosperms examined possessed: beta,beta-carotene, lutein, zeaxanthin, violaxanthin, neoxanthin and other minor pigments. Two unidentified pigments with the same retention time as two "siphonaxanthin-type" pigments were isolated from the green alga Flabella petiolata and are relatively abundant in the species P. oceanica and H. stipulacea. Pigment data indicate a possible biochemical adaptation to different light regimes in seagrass species that can colonize deeper habitats. [References: 44] LG - English PT - Article SB - Current Contents(R)/Agriculture, Biology & Environmental Sciences CC - Aquatic Sciences in Current Contents(R)/Agricultural, Biology & Environmental Sciences. EW - 2003 week 31 IN - Reprint available from: Casazza G APAT Via Vitaliano Brancati 48 I-00144 Rome Italy Stazione Zoolog A Dohrn, Lab Ecol Benthos I-80077 Ischia Italy <40> UI - 697PR-0013 DD - ISI Document Solution: 697PR AU - Ito Y AU - Asayama M AU - Shirai M MA - asam@mx.ibaraki.ac.jp RA - Asayama M TI - Light-responsive psbA transcription requires the-35 hexamer in the promoter and its proximal upstream element, UPE, in cyanobacteria SO - Bioscience Biotechnology & Biochemistry. 67(6):1382-1390, 2003 Jun. AS - Biosci. Biotechnol. Biochem 2003 Jun;67(6):1382-1390 PU - JAPAN SOC BIOSCI BIOTECHN AGROCHEM, JAPAN ACAD SOC CTR BLDG, 2-4-6 YAYOI BUNKYO-KU, TOKYO, 113, JAPAN. URL: http://wwwsoc.nacsis.ac.jp/jsbba IS - 0916-8451 MH - Transcription MH - Rna polymerase MH - Sigma factor MH - Curved dna MH - Photosynthesis. MH - Principal sigma-factor MH - Microcystis-aeruginosa k-81 MH - Sp strain pcc-7120 MH - Rna-polymerase MH - Evolutionary relationships MH - Gene-expression MH - Sequence MH - Curvature MH - Family. AB - We characterized the function of the -35 hexamer in the promoter and an element just upstream, UPE, in the expression in a unicellular cyanobacterium, Microcystis aeruginosa K-81, of the light-responsive gene psbA2, which encodes a reaction center key protein for photosynthesis. A series of mutants with mutations at the -35 hexamer (-35 to -30) and a novel conserved upstream element (UPE: -45 to -36, +1 referring to the transcription start point) were constructed. Expression of the mutants was examined in vivo and in vitro by analyses using a beta-galactosidase assay, primer extension, and a DNA-mobility shift assay with RNA polymerases. Results indicated that the -35 hexamer and its proximal UPE act as effective cis-elements for the light-responsive and/or basal transcription, respectively. A model of the 5'-upstream region with cis- and possible trans-acting factors is presented for the psbA regulatory system. [References: 30] LG - English PT - Article SB - Current Contents(R)/Agriculture, Biology & Environmental Sciences Current Contents(R)/Life Sciences CC - Agricultural Chemistry in Current Contents(R)/Agricultural, Biology & Environmental Sciences. Biochemistry & Biophysics in Current Contents(R)/Life Sciences. EW - 2003 week 31 IN - Reprint available from: Asayama M Ibaraki Univ, Sch Agr, Genet Mol Lab Ibaraki 3000393 Japan Ibaraki Univ, Sch Agr, Genet Mol Lab Ibaraki 3000393 Japan <41> UI - 697MW-0029 DD - ISI Document Solution: 697MW AU - Yabunaka H AU - Abe M AU - Kenmochi A AU - Hamada T AU - Nishioka T AU - Miyoshi H MA - miyoshi@kais.kyoto-u.ac.jp RA - Miyoshi H TI - Synthesis and inhibitory activity of ubiquinone-acetogenin hybrid inhibitor with bovine mitochondrial complex I SO - Bioorganic & Medicinal Chemistry Letters. 13(14):2385-2388, 2003 Jul 21. AS - Bioorg. Med. Chem. Lett 2003 Jul 21;13(14):2385-2388 PU - PERGAMON-ELSEVIER SCIENCE LTD, THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND. URL: http://www.elsevier.nl IS - 0960-894X MH - Essential structural factors MH - Annonaceous acetogenins MH - Potent inhibitors MH - Difference spectroscopy MH - Reduction site MH - Binding-sites MH - Oxidoreductase MH - Subunit MH - Quinone MH - Design. AB - To elucidate the inhibitory action of acetogenins, the most potent inhibitors of mitochondrial complex I, we synthesized an acetogenin analogue which possesses a ubiquinone ring (i.e., the physiological substrate of complex I) in place of the alpha,beta-unsaturated gamma-lactone ring of natural acetogenins, and named it Q-acetogenin. Our results indicate that the gamma-lactone ring of acetogenins is completely substitutable with the ubiquinone ring. This fact is discussed in light of the inhibitory action of acetogenins. (C) 2003 Elsevier Science Ltd. All rights reserved. [References: 19] LG - English PT - Article SB - Current Contents(R)/Life Sciences CC - Chemistry & Analysis in Current Contents(R)/Life Sciences. EW - 2003 week 31 IN - Reprint available from: Miyoshi H Kyoto Univ, Grad Sch Agr, Div Appl Life Sci, Sakyo Ku Kyoto 6068502 Japan Kyoto Univ, Grad Sch Agr, Div Appl Life Sci, Sakyo Ku Kyoto 6068502 Japan <42> UI - 697RW-0021 DD - ISI Document Solution: 697RW AU - Zhang CX AU - Styring S MA - Stenbjorn.Styring@biokem.lu.se RA - Styring S TI - Formation of split electron paramagnetic resonance signals in photosystem II suggests that tyrosine(z) can be photooxidized at 5 K in the S-0 and S-1 states of the oxygen-evolving complex SO - Biochemistry. 42(26):8066-8076, 2003 Jul 8. AS - Biochemistry 2003 Jul 8;42(26):8066-8076 PU - AMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 USA. URL: http://pubs.acs.org IS - 0006-2960 MH - Photosynthetic water oxidation MH - Epr multiline signal MH - Ftir difference spectroscopy MH - S-1-state manganese cluster MH - Amino-acid-residues MH - Y-z MH - Temperature-dependence MH - S2yz-center-dot state MH - Enzymatic catalysis MH - Histidine oxidation. AB - The effect of illumination at 5 K of photosystem II in different S-states was investigated with EPR spectroscopy. Two split radical EPR signals around g approximate to 2.0 were observed from samples given 0 and 3 flashes, respectively. The signal from the 0-flash sample was narrow, with a width of similar to80 G, in which the low-field peak can be distinguished. This signal oscillated with the S, state in the sample. The signal from the 3-flash sample was broad, with a symmetric shape of similar to160 G width from peak to trough. This signal varied with the concentration of the So state in the sample. Both signals are assigned to arise from the donor side of PSII Both signals relaxed fast, were formed within 10 ms after a flash, and decayed with half-times at 5 K of 3-4 min. The signal in the So state closely resembles split radical signals, originating from magnetic interaction between Y-Z(.) and the S-2 state, that were first observed in Ca2+-depleted photosystem II samples. Therefore, we assign this signal to Y-Z(.) in magnetic interaction with the S-0 state, Yz(.)S(0). The other signal is assigned to the magnetic interaction between Y-Z(.) and the S-1 state, (YZS1)-S-.. An important implication is that Y-Z can be oxidized at 5 K in the S-0 and S-1 states. Oxidation of Y-Z involves deprotonation of the tyrosine. This is restricted at 5 K, and we therefore suggest that the phenolic proton of Y-Z is involved in a low-barrier hydrogen bond. This is an unusually short hydrogen bond in which proton movement at very low temperatures can occur. [References: 89] LG - English PT - Article SB - Current Contents(R)/Life Sciences CC - Biochemistry & Biophysics in Current Contents(R)/Life Sciences. EW - 2003 week 31 IN - Reprint available from: Styring S Lund Univ, Dept Biochem, Ctr Chem & Chem Engn POB 124 S-22100 Lund Sweden Lund Univ, Dept Biochem, Ctr Chem & Chem Engn S-22100 Lund Sweden <43> UI - 695KJ-0007 DD - ISI Document Solution: 695KJ AU - Ala-Rami A AU - Ylitalo KV AU - Hassinen IE MA - ilmo.hassinen@oulu.fi RA - Hassinen IE TI - Ischaemic preconditioning and a mitochondrial K-ATP channel opener both produce cardioprotection accompanied by F1F0-ATPase inhibition in early ischaemia SO - Basic Research in Cardiology. 98(4):250-258, 2003 Jul. AS - Basic Res. Cardiol 2003 Jul;98(4):250-258 PU - DR DIETRICH STEINKOPFF VERLAG, PO BOX 10 04 62, D-64204 DARMSTADT, GERMANY. URL: http://www.steinkopff.springer.de IS - 0300-8428 MH - Ischaemia MH - Preconditioning MH - K-atp channel MH - Atp synthase diazoxide. MH - High-energy phosphates MH - Potassium channels MH - Rat hearts MH - Sodium 5-hydroxydecanoate MH - Possible mechanism MH - Intracellular ph MH - Ca2+ overload MH - Redox state MH - Reperfusion MH - Myocardium. AB - Ischaemic preconditioning gives powerful protection against prolonged ischaemia affecting several intracellular regulatory and messenger pathways, although their mutual importance is far from established. Protective, preconditioning-like effects have been reported for K-ATP channel openers, and most of the evidence points to the mitochondrial K-ATP channels. We show here that the K-ATP channel opener diazoxide, which acts selectively on the mitochondrial channel, causes potentiation of ischaemic inhibition of mitochondrial ATP synthase (F1F0-ATPase) along with cardioprotection. These effects are comparable with that of ischaemic preconditioning. The administration of diazoxide did not affect the cellular energy state as monitored with P-31 NMR. The actions of both diazoxide and ischaemic preconditioning were prevented by 5-hydroxydecanoate, a specific inhibitor of the mitochondrial K-ATP channel. Thus mitochondrial K-ATP channel opening and ischaemic preconditioning must share common mechanisms of action involving mitochondrial F1F0-ATPase, although involvement of the energy state in protection could not be proved. [References: 47] LG - English PT - Article SB - Current Contents(R)/Life Sciences CC - Cardiovascular & Hematology Research in Current Contents(R)/Life Sciences. EW - 2003 week 31 IN - Reprint available from: Hassinen IE Univ Oulu, Dept Med Biochem & Mol Biol POB 5000 Oulu 90014 Finland Univ Oulu, Dept Med Biochem & Mol Biol Oulu 90014 Finland Univ Oulu, Dept Internal Med Oulu 90014 Finland <44> UI - 698CN-0005 DD - ISI Document Solution: 698CN AU - Wang KY AU - Kellomaki S AU - Li CY AU - Zha TS MA - kaiyun@joensuu.fi RA - Wang KY TI - Light and water-use efficiencies of pine shoots exposed to elevated carbon dioxide and temperature SO - Annals of Botany. 92(1):53-64, 2003 Jul. AS - Ann. Bot 2003 Jul;92(1):53-64 PU - OXFORD UNIV PRESS, GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND. URL: http://www.oup.co.uk IS - 0305-7364 MH - Carbon flux MH - Water flux MH - Elevated co2 MH - Elevated temperature MH - Water-use efficiency MH - Light-use efficiency MH - Environment-controlled chamber MH - Pinus sylvestris MH - Scots pine. MH - Scots pine MH - Atmospheric co2 MH - Chlorophyll fluorescence MH - Stomatal conductance MH - Gas-exchange MH - Needle age MH - Photosynthesis MH - Growth MH - Field MH - Seedlings. AB - An automatic gas exchange system was used to continuously measure water and carbon fluxes of attached shoots of Scots pine trees (Pinus sylvestris L.) grown in environment-controlled chambers for a 3-year period (1998-2000) and exposed to either normal ambient conditions (CON), elevated CO2 (+350 mumol mol(-1); EC), elevated temperature (+2-6 degreesC; ET) or a combination of EC and ET (ECT). EC treatment enhanced the mean daily total carbon flux per unit projected needle area (Fc(.d)) by 17-21 %, depending on the year. This corresponds to a 16-24 % increase in light-use efficiency (LUE) based on incident photosynthetically active radiation. The EC treatment reduced the mean daily total water flux (F-w.d) by 1-12 %, corresponding to a 13-35 % increase in water-use efficiency (WUE). The ET treatment increased F-c.d by 10-18 %, resulting in an 8-19 % increase in LUE, and Fw(.d) by 48-74 %, resulting in a reduction of WUE by 19-34 %. There was no interaction between CO2 and temperature elevation in connection with either carbon or water fluxes, as the carbon flux responded similarly in both ECT and EC, while the water flux in the ECT treatment was similar to that in ET. Regressions indicated that the increase in maximum LUE was greater with increasing air temperature, whereas changes in WUE were related only to high vapour pressure deficit. Furthermore, changes in LUE and WUE caused by ECT treatment displayed strong diurnal and seasonal variation. (C) 2003 Annals of Botany Company. [References: 34] LG - English PT - Article SB - Current Contents(R)/Agriculture, Biology & Environmental Sciences CC - Plant Sciences in Current Contents(R)/Agricultural, Biology & Environmental Sciences. EW - 2003 week 31 IN - Reprint available from: Wang KY Univ Joensuu, Ctr Excellence Forest Ecosyst & Management POB 11 FIN-80101 Joensuu Finland Univ Joensuu, Ctr Excellence Forest Ecosyst & Management FIN-80101 Joensuu Finland Chinese Acad Sci, Chengdu Inst Biol Chengdu 610041 Peoples R China <45> UI - 698AA-0006 DD - ISI Document Solution: 698AA AU - Majernik A AU - Cubonova L AU - Polak P AU - Smigan P AU - Greksak M RA - Smigan P TI - Biochemical analysis of neomycin-resistance in the methanoarchaeon Methanothermobacter thermautotrophicus and some implications for energetic processes in this strain SO - Anaerobe. 9(1):31-38, 2003 Feb. AS - Anaerobe 2003 Feb;9(1):31-38 PU - ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD, 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND. URL: http://www.apnet.com IS - 1075-9964 MH - Neomycin-resistant mutant MH - Membrane-bound protein alternations MH - A(1)a(0) atpase/synthase MH - Na+/h+ antiport MH - Membrane potential. MH - Complete genome sequence MH - Methanobacterium-thermoautotrophicum MH - Methanogenic archaea MH - Methanococcus-jannaschii MH - Methanosarcina-mazei MH - Transport system MH - Na+/h+ antiport MH - Delta-h MH - Driven MH - Atpase. AB - Methanogenesis-driven ATP synthesis in a neomycin-resistant mutant of Methanothermobacter thermautotrophicus (formerly Methanobacterium thermoautotrophicum strain DeltaH) was strongly inhibited at both pH 6.8 and pH 8.5 by the uncoupler 3,3',4',5 tetrachorosalicylanilide (TCS) in the presence of either I or 10mM NaCl. The generation of a membrane potential in the mutant cells at pH 6.8 was also strongly inhibited by TCS in the presence of I or I 10mM NaCl. On the other hand, at pH 8.5 in the presence of 10mM NaCl, a protonophore-resistant membrane potential of approximately 150mV was found. These results indicate that in the mutant cells the process of energy transduction between methanogenesis and membrane potential generation is not impaired. In contrast to the wild-type strain, ATP synthesis in the mutant cells was driven by an electrochemical gradient of H+ under alkaline conditions. Unlike wild-type cells, the mutant lacks the capacity to transduce an uncoupler-resistant membrane potential energy at pH 8.5 into ATP synthesis. Na+/H+ exchange was comparable in the wild type and the mutant cells. Western blots of sub-cellular fractions with polyclonal antiserum reactive to the B-subunit of the halobacterial A-type H+-translocating ATPase confirmed the presence of A-type ATP synthase in the mutant cells. Furthermore, in the mutant cells a protein band of molecular mass about 45 kDa is absent but there was an abundant protein band at about 67 kDa. Based on the observed bioenergetic features of the mutant cells, neither the A(1)A(0) ATP synthase alone nor together with the Na+/H+ antiporter seems to be responsible for ATP synthesis driven by sodium motive force. Rather, some other links between neomycin-resistance and failure of sodium motive force-dependent ATP synthesis in the neomycin resistant mutant are discussed. (C) 2003 Elsevier Science Ltd. All rights reserved. [References: 31] LG - English PT - Article SB - Current Contents(R)/Agriculture, Biology & Environmental Sciences Current Contents(R)/Life Sciences CC - Biology in Current Contents(R)/Agricultural, Biology & Environmental Sciences. Microbiology in Current Contents(R)/Life Sciences. EW - 2003 week 31 IN - Reprint available from: Smigan P Slovak Acad Sci, Inst Anim Biochem & Genet Ivanka Pri Dunaji S0028 Slovakia Slovak Acad Sci, Inst Anim Biochem & Genet Ivanka Pri Dunaji S0028 Slovakia <46> UI - 696DC-0003 DD - ISI Document Solution: 696DC AU - Rapacz M AU - Waligorski P AU - Janowiak F MA - rrrapacz@cyf-kr.edu.pl RA - Rapacz M TI - ABA and gibberellin-like substances during prehardening, cold acclimation, de- and reacclimation of oilseed rape SO - Acta Physiologiae Plantarum. 25(2):151-161, 2003. AS - Acta Physiol. Plant 2003;25(2):151-161 PU - POLISH ACAD SCIENCES DEPT PLANT PHYSIOLOGY, SLAWKOWSKA STREET NO 17, PL31-016 KRAKOW, POLAND IS - 0137-5881 MH - Abscisic acid MH - Brassica napus var. oleifera MH - Cold acclimation MH - Cold deacclimation MH - Gibberellins MH - Growth rate MH - Prehardening MH - Psii excitation pressure. MH - Napus var. oleifera MH - Stress signaling pathways MH - Cv gorczanski seedlings MH - Brassica-napus MH - Low-temperature MH - Abscisic-acid MH - Frost-resistance MH - Xanthophyll cycle MH - De-acclimation MH - Early growth. AB - Previously published results showed that high relative reduction state of PSII (PSII excitation pressure) during both early seedling growth (prehardening) as well as cold deacclimation caused significant changes in growth pattern. The differences in elongation growth rate were related to the cold acclimation of photosynthetic apparatus and to frost resistance. To study changes in the hormonal balance connected with alterations in elongation growth rate observed during prehardening and deacclimation under different PSII excitation pressure (modulated by day-temperatures), endogenous concentration of ABA, GA(3) and GA-like substances (GAs) were analysed. Analyses were also performed during cold acclimation and reacclimation of plants characterized by different elongation growth rate triggered by prehardening or deacclimation under different day-temperatures. Growth under high PSII excitation pressure (prehardening) resulted in a significant increase in ABA and a considerable decrease in GAs contents. On the other hand, different ABA content played almost no role in controlling growth rate during cold deacclimation and subsequent reacclimation, when the induction of elongation growth was connected with the changes in concentration of GAs including GA(3). The possible role of ABA and GAs in controlling prehardening, cold acclimation and deacclimation is discussed. [References: 39] LG - English PT - Article SB - Current Contents(R)/Agriculture, Biology & Environmental Sciences CC - Plant Sciences in Current Contents(R)/Agricultural, Biology & Environmental Sciences. EW - 2003 week 31 IN - Reprint available from: Rapacz M Agr Univ Cracow, Fac Agr & Econ, Dept Plant Physiol Krakow Poland Agr Univ Cracow, Fac Agr & Econ, Dept Plant Physiol Krakow Poland Polish Acad Sci, Franciszek Gorski Dept Plant Physiol PL-30239 Krakow Poland <47> UI - 696RF-0011 DD - ISI Document Solution: 696RF AU - Cunha MA AU - Almeida MA AU - Alcantara F MA - acunha@bio.ua.pt RA - Cunha MA TI - Ectoenzymatic activity and glucose heterotrophic metabolism in a shallow estuary (Ria de Aveiro, Portugal): influence of bed sediments and salt marshes SO - Acta Oecologica-International Journal of Ecology. 24(Suppl 1):S97-S107, 2003 May. AS - Acta Oecol.-Int. J. Ecol 2003 May;24(Suppl 1):S97-S107 PU - GAUTHIER-VILLARS/EDITIONS ELSEVIER, 23 RUE LINOIS, 75015 PARIS, FRANCE. URL: http://www.gauthier-villars.fr IS - 1146-609X MH - Estuarine bacterioplankton MH - Ectoenzymatic activity MH - Heterotrophic activity MH - Sediment resuspension. MH - Outwelling hypothesis MH - Resuspension MH - Bacteria MH - Gradient MH - Fluorescence MH - Chlorophyll MH - Environment MH - Abundance MH - Turnover MH - Patterns. AB - Bacterioplankton abundance and activity were studied in the estuarine system of Ria de Aveiro (Portugal) to test if tidal resuspension of sediments and transport of particles from the salt marshes may act as factors of variability of bacterial communities. The total and attached cell abundance, ectoenzymatic activity and the heterotrophic metabolism of glucose, as well as seston, chlorophyll alpha and particulate organic carbon (POC) were monitored during four 10-h periods along the tidal cycle at four sampling sites across a transect. The variation of particulate materials (seston, POC and chlorophyll alpha) along the transect was not significantly correlated with either distance to the margin or distance to the sediment surface. Nevertheless, proximity to the salt marsh or to the bottom sediment surface favoured glucose incorporation and aminopeptidase activity. A multiple stepwise linear regression analysis using temperature, salinity, seston, POC, chlorophyll a, distance to sediment surface and distance to the margin as independent variables explained 66.5% of the variability of the fraction of particle-attached bacteria and only a very small proportion (12-43%) of the observed variability of total bacterial abundance, ectoenzymatic activity and glucose utilization. The spatial patterns of variation of the concentration of particulate material (seston, POC and chlorophyll alpha) do not clearly indicate the occurrence of sediment resuspension and runoff from the margins. This, together with the poor contribution of these parameters to the transversal and tidal variability of bacterial activity, dismisses the importance of inputs of suspended material across the sediment/water interface and from neighbouring salt marshes in the control of bacterial density and activity. (C) 2003 Editions scientifiques et medicales Elsevier SAS. All rights reserved. [References: 34] LG - English PT - Article SB - Current Contents(R)/Agriculture, Biology & Environmental Sciences CC - Environment/Ecology in Current Contents(R)/Agricultural, Biology & Environmental Sciences. EW - 2003 week 31 IN - Reprint available from: Cunha MA Univ Aveiro, Dept Biol Campo Univ Santiago P-3810 Aveiro Portugal Univ Aveiro, Dept Biol P-3810 Aveiro Portugal