<1> UI - 702AW-0003 DD - ISI Document Solution: 702AW AU - Konovalova NV AU - Evstigneeva RP AU - Luzgina VN MA - httos.mitht@g23.relcom.ru RA - Konovalova NV TI - Synthetic molecular systems based on porphyrins as models for study of energy transfer in photosynthesis [Review] [Russian] SO - Uspekhi Khimii. 70(11):1059-1093, 2001. AS - Uspekhi Khimii 2001;70(11):1059-1093 PU - MEZHDUNARODNAYA KNIGA, 39 DIMITROVA UL., 113095 MOSCOW, RUSSIA. URL: http://www.maik.rssi.ru IS - 0042-1308 MH - Light-harvesting arrays MH - Covalently-linked porphyrin MH - Intramolecular electron-transfer MH - Excited-state interactions MH - Primary charge separation MH - Reaction centers MH - Multiporphyrin arrays MH - Photoinduced energy MH - Tetraphenylporphyrin derivatives MH - Fucoxanthin-pyropheophorbide. AB - Data on the synthesis and photochemical properties of molecular porphyrin-based ensembles, which are models of natural photosynthetic light-harvesting complexes, are reviewed. The dependence of excitation energy transfer on the distance between, and the mutual orientation of, donor and acceptor, on the electronic factors and medium parameters are discussed. Two mechanisms of the energy transfer, through space and through bond, are considered. [References: 94] LG - Russian PT - Review SB - Current Contents(R)/Physical, Chemical & Earth Sciences CC - Chemistry in Current Contents(R)/Physical, Chemical & Earth Sciences. EW - 2003 week 33 IN - Reprint available from: Konovalova NV MV Lomonosov Moscow State Acad Fine Chem Technol 86 Prosp Vernadskogo Moscow 117571 Russia MV Lomonosov Moscow State Acad Fine Chem Technol Moscow 117571 Russia <2> UI - 701GV-0008 DD - ISI Document Solution: 701GV AU - Willems A AU - Munive A AU - de Lajudie P AU - Gillis M MA - anne.willems@rug.ac.be RA - Willems A TI - In most Bradyrhizobium groups sequence comparison of 16S-23S rDNA internal transcribed spacer regions corroborates DNA-DNA hybridizations SO - Systematic & Applied Microbiology. 26(2):203-210, 2003 Jun. AS - Syst. Appl. Microbiol 2003 Jun;26(2):203-210 PU - URBAN & FISCHER VERLAG, BRANCH OFFICE JENA, P O BOX 100537, D-07705 JENA, GERMANY. URL: http://www.urbanfischer.de IS - 0723-2020 MH - Its MH - Bradyrhizobium MH - Dna-dna hybridizations. MH - Ribosomal-rna gene MH - Sp-nov MH - Blastobacter-denitrificans MH - Soybean bradyrhizobia MH - Phylogenetic analysis MH - Strains MH - Differentiation MH - Japonicum MH - Rhizobia MH - Identification. AB - In an extension of a previous small-scale test to assess the use of 16S-23S rDNA internal transcribed spacer (ITS) sequences for rapid grouping of bradyrhizobia, we have sequenced the ITS region of 32 isolates of Bradyrhizobium that had previously been studied using AFLP and DNA-DNA hybridizations. We also included representatives of Afipia and Rhodopseudomonas. Our results indicate that ITS sequences are very diverse among bradyrhizobia. Nevertheless, for most of the bradyrhizobia, the grouping of ITS sequences was in line with AFLP results and DNA-DNA hybridization data. Strains that have at least 95.5% ITS sequence similarity belong to the same genospecies, i.e. they have more than 60% DNA-DNA hybridization values. The ITS sequences can therefore provide a relatively fast way to guide strain identification and aid selection of the reference groups that should be included in DNA-DNA hybridization experiments for precise genotypic identification. The Bradyrhizobium strains isolated from Aeschynomene species showed a much larger diversity in ITS sequences than other bradyrhizobia, possibly as a result of lateral exchange. The above ITS sequence similarity criterion for genospecies therefore does not apply to them, but they can easily be distinguished from other Bradyrhizobium genospecies because they have a distinct tRNA(ala) gene. [References: 27] LG - English PT - Article SB - Current Contents(R)/Life Sciences CC - Microbiology in Current Contents(R)/Life Sciences. EW - 2003 week 33 IN - Reprint available from: Willems A State Univ Ghent, Microbiol Lab, Dept Physiol Biochem & Microbiol, Fac Sci KL Ledeganckstr 35 B-9000 Ghent Belgium State Univ Ghent, Microbiol Lab, Dept Physiol Biochem & Microbiol, Fac Sci B-9000 Ghent Belgium Lab Symbioses Trop Mediterraneennes Montpellier France <3> UI - 703CK-0003 DD - ISI Document Solution: 703CK AU - Seppanen M AU - Turakainen M AU - Hartikainen H MA - mervi.seppanen@helsinki.fi RA - Seppanen M TI - Selenium effects on oxidative stress in potato SO - Plant Science. 165(2):311-319, 2003 Aug. AS - Plant Sci 2003 Aug;165(2):311-319 PU - ELSEVIER SCI IRELAND LTD, CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND. URL: http://www.elsevier.nl IS - 0168-9452 MH - Potato MH - Low temperature stress MH - Oxidative stress MH - Photoinhibition. MH - Superoxide-dismutase MH - Cold-acclimation MH - Glutathione-peroxidase MH - Gene-expression MH - Dietary selenium MH - Uv irradiation MH - Messenger-rna MH - Redox control MH - Tolerance MH - Light. AB - Higher plants are considered not to require selenium (Se). However, it has recently been shown that Se increases the antioxidative capacity and stress tolerance of lettuce (Lactuca sativa L.) and ryegrass (Lolium perenne L.). This research was undertaken to investigate the antioxidative properties of Se during photooxidative stress in potato (Solanum tuberosum L.) and to determine the defence mechanisms. Potato plants were exposed to 600 mumol/m(2)/s light intensity at low temperature (4 degreesC) or paraquat-mediated oxidative stress. The stress responses were monitored by measuring chlorophyll content and following changes in chlorophyll fluorescence and membrane ion leakage. Moreover, the effects of Se on the transcript levels of chloroplast CuZnSOD, mitochondrial MnSOD, glutathione peroxidase (GPX), and psbA were analyzed using northern hybridization. Se supplementation improved the recovery of chlorophyll content following light stress. After prolonged exposure to light, the reduction of Fv/Fm was slightly lower compared with plants cultivated without additional Se. The photosynthesis of Se treated plants was somewhat more tolerant of paraquat and the integrity of membranes was improved during oxidative stress. Se altered transcript accumulation of chloroplast CuZnSOD and GPX but the MnSOD and psbA transcript levels were unaffected. The results suggest that Se is an antioxidant or it activates protective mechanisms, which can alleviate oxidative stress in the chloroplasts. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved. [References: 55] LG - English PT - Article SB - Current Contents(R)/Agriculture, Biology & Environmental Sciences Current Contents(R)/Life Sciences CC - Plant Sciences in Current Contents(R)/Agricultural, Biology & Environmental Sciences. Animal & Plant Sciences in Current Contents(R)/Life Sciences. EW - 2003 week 33 IN - Reprint available from: Seppanen M Univ Helsinki, Dept Appl Biol POB 27 FIN-00014 Helsinki Finland Univ Helsinki, Dept Appl Biol FIN-00014 Helsinki Finland Univ Helsinki, Dept Appl Chem & Microbiol FIN-00014 Helsinki Finland Univ Maryland, Dept Nat Resource Sci & Landscape Architecture College Pk, MD 20742 USA <4> UI - 701UC-0023 DD - ISI Document Solution: 701UC AU - Dreuw A AU - Fleming GR AU - Head-Gordon M MA - andreas@bastille.cchem.berkeley.edu RA - Dreuw A TI - Chlorophyll fluorescence quenching by xanthophylls SO - Physical Chemistry Chemical Physics. 5(15):3247-3256, 2003. AS - Phys. Chem. Chem. Phys 2003;5(15):3247-3256 PU - ROYAL SOC CHEMISTRY, THOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLAND. URL: http://www.rsc.org IS - 1463-9076 MH - Density-functional theory MH - Correct asymptotic-behavior MH - Light-harvesting complex MH - Carotenoid s-1 state MH - Energy-transfer MH - Green plants MH - Excitation-spectra MH - Photosystem-ii MH - Cycle MH - Zeaxanthin. AB - The interactions of the xanthophylls zeaxanthin, antheraxanthin and violaxanthin with chlorophyll, which are relevant for the chlorophyll fluorescence quenching in the non-photochemical quenching (NPQ) process in green plants, are investigated by means of quantum chemical methods. In particular, we use a hybrid approach consisting of time-dependent density-functional theory (TDDFT) and configuration interactions singles (CIS), since present-day TDDFT alone fails in describing relevant long-range charge-transfer states. Calculation of the energetically lowest excited states of a zea-chl dimer along the intermolecular distance coordinate shows that two mechanisms for chlorophyll fluorescence quenching are possible: quenching via excitation-energy transfer and electron-transfer quenching. The relevance of both mechanisms is discussed by comparison to corresponding anthera-chl and vio-chl dimers and the dependence of the mechanism on the geometrical arrangement of the dimers is illuminated. It is pointed out that the typical absorption band of the zeaxanthin radical cation can be used to experimentally determine whether a zea-chl dimer is present during NPQ. [References: 51] LG - English PT - Article SB - Current Contents(R)/Physical, Chemical & Earth Sciences CC - Physical Chemistry/Chemical Physics in Current Contents(R)/Physical, Chemical & Earth Sciences. EW - 2003 week 33 IN - Reprint available from: Dreuw A Univ Calif Berkeley, Dept Chem Berkeley, CA 94720 USA Univ Calif Berkeley, Dept Chem Berkeley, CA 94720 USA Univ Calif Berkeley, Lawrence Berkeley Lab, Chem Sci & Phys Biosci Div Berkeley, CA 94720 USA <5> UI - 701FY-0008 DD - ISI Document Solution: 701FY AU - Mobius K RA - Mobius K TI - Multifrequency EPR and ENDOR on transient radicals and radical pairs in liquid crystals and proteins: Structure and dynamics of cofactors in natural and artifical photosynthesis SO - Molecular Crystals & Liquid Crystals. 394(Part 1):1-17, 2003. AS - Mol. Cryst. Liquid Cryst 2003;394(Part 1):1-17 PU - TAYLOR & FRANCIS LTD, 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND. URL: http://www.tandf.co.uk IS - 1058-725X MH - Time-resolved epr MH - Electron transfer MH - Photosynthesis MH - Model system. MH - High-field epr MH - Electron-paramagnetic-resonance MH - Bacterial reaction centers MH - Ftir difference spectroscopy MH - Axially-symmetrical solutes MH - Rhodobacter-sphaeroides r26 MH - 95 ghz MH - Model systems MH - Quadrupole splittings MH - Rotational-dynamics. AB - Hotly debated issues in the area of biological electron transfer (ET) involve questions of how long-range ET processes proceed along pathways that incorporate non-covalently linked cofactors interacting with their protein microenvironment. To contribute answers to such questions, time-resolved EPR (TREPR) experiments at various microwave frequency/magnetic field settings have been performed. In this overview, representative examples of this work from our laboratory are given which comprise transient intermediates of light-induced ET in (i) bacterial photosynthetic reaction centers and (ii) biomimetic model complexes. In these complexes the donor and acceptor components are tethered together either covalently via cyclohexenyl bridges or non-covalently via Watson-Crick base-pairing. The studies are aiming at broadening our knowledge of structure-dynamics-function relationships associated with ET processes. It is demonstrated that high-field TREPR opens new perspectives in elucidating complex photochemical ET reactions with different paramagnetic states and species involved. [References: 47] LG - English PT - Article SB - Current Contents(R)/Physical, Chemical & Earth Sciences CC - Physical Chemistry/Chemical Physics in Current Contents(R)/Physical, Chemical & Earth Sciences. EW - 2003 week 33 IN - Reprint available from: Mobius K Free Univ Berlin, Dept Phys Arnimallee 14 D-14195 Berlin Germany Free Univ Berlin, Dept Phys D-14195 Berlin Germany <6> UI - 701FY-0009 DD - ISI Document Solution: 701FY AU - Di Valentin M AU - Bisol A AU - Giacometti G AU - Carbonera D AU - Agostini G AU - Liddell PA AU - Moore AL AU - Moore TA AU - Gust D MA - m.divalentin@chfi.unipd.it RA - Di Valentin M TI - Reaction center models in liquid crystals: Identification of paramagnetic intermediates SO - Molecular Crystals & Liquid Crystals. 394(Part 1):19-30, 2003. AS - Mol. Cryst. Liquid Cryst 2003;394(Part 1):19-30 PU - TAYLOR & FRANCIS LTD, 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND. URL: http://www.tandf.co.uk IS - 1058-725X MH - Photosynthetic model system MH - Electron transfer MH - Liquid crystals : epr. MH - Photoinduced electron-transfer MH - Porphyrin-fullerene triad MH - Energy-transfer MH - Triplet-state MH - Photosynthesis MH - Separation MH - Charge MH - Epr. AB - Photoinduced charge separation and recombination to the triplet state in a carotene (C) porphyrin (P) fullerene (C-60) triad have been followed by time-resolved electron paramagnetic resonance (EPR). The electron transfer process has been studied at different temperatures in both a glassy isotropic matrix (2-methyltetrahydrofuran) and uniaxial liquid crystal (E-7). In both media, the triad undergoes two-step photoinduced electron transfer with the generation of a long-lived charge separated state (C.+-P-C-60(.-)), and charge recombination to the triplet state, localized in the carotene moiety. The carotenoid triplet state is initially polarized according to the mechanism of recombination of a radical pair with singlet precursor. Both the photoinduced spin-correlated radical pair and the carotene triplet are observed in E-7 starting from the glass to the liquid crystal phase. The exchange interaction between the electrons in the radical pair (J = 1.2 Gauss) has been evaluated by simulation of the EPR spectrum in the isotropic glass and comparison with the corresponding spectrum in the oriented medium. [References: 17] LG - English PT - Article SB - Current Contents(R)/Physical, Chemical & Earth Sciences CC - Physical Chemistry/Chemical Physics in Current Contents(R)/Physical, Chemical & Earth Sciences. EW - 2003 week 33 IN - Reprint available from: Di Valentin M Univ Padua, Dipartimento Chim Fis Via Loredan 2 I-35131 Padua Italy Univ Padua, Dipartimento Chim Fis I-35131 Padua Italy CNR, Ist Chim Biomol, Sez Padova I-35131 Padua Italy Arizona State Univ, Dept Chem & Biochem, Ctr Study Early Events Photosynthesis Tempe, AZ 85287 USA <7> UI - 701WD-0018 DD - ISI Document Solution: 701WD AU - Lankin VZ AU - Tikhaze AK AU - Kukharchuk VV AU - Konovalova GG AU - Pisarenko OI AU - Kaminnyi AI AU - Shumaev KB AU - Belenkov YN MA - lankin@vipmail.ru RA - Lankin VZ TI - Antioxidants decreases the intensification of low density lipoprotein in vivo peroxidation during therapy with statins SO - Molecular & Cellular Biochemistry. 249(1-2):129-140, 2003 Jul. AS - Mol. Cell. Biochem 2003 Jul;249(1-2):129-140 PU - KLUWER ACADEMIC PUBL, VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS. URL: http://www.wkap.nl IS - 0300-8177 MH - Hmg-coa-reductase inhibitors MH - Vitamin e MH - Ubiquinone q(10) MH - Probucol MH - Ldl free radical peroxidation MH - Lipohydroperoxides. MH - Dependent dehydroascorbate reductase MH - Radical lipid-peroxidation MH - Coenzyme-q levels MH - Ldl oxidation MH - Rat-liver MH - Ex-vivo MH - Probucol MH - Atherosclerosis MH - Blood MH - Reticulocytes. AB - The oxidative modification of low density lipoprotein (LDL) is thought to play an important role in atherogenesis. Drugs of beta-hydroxy- beta-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitors (statins) family are usually used as a very effective lipid-lowering preparations but they simultaneously block biosynthesis of both cholesterol and ubiquinone Q(10) ( coenzyme Q), which is an intermediate electron carrier in the mitochondrial respiratory chain. It is known that reduced form of ubiquinone Q(10) acts in the human LDL as very effective natural antioxidant. Daily per os administration of HMG-CoA reductase inhibitor simvastatin to rats for 30 day had no effect on high-energy phosphates ( adenosin triphosphate, creatine phosphate) content in liver but decreased a level of these substances in myocardium. We study the Cu2+-mediated susceptibility of human LDL to oxidation and the levels of free radical products of LDL lipoperoxidation in LDL particles from patients with atherosclerosis after 3 months treatment with natural antioxidants vitamin E as well as during 6 months administration of HMG-CoA reductase inhibitors such as pravastatin and cerivastatin in monotherapy and in combination with natural antioxidant ubiquinone Q(10) or synthetic antioxidant probucol in a double-blind placebo-controlled trials. The 3 months of natural antioxidant vitamin E administration ( 400 mg daily) to patients did not increase the susceptibility of LDL to oxidation. On the other hand, synthetic antioxidant probucol during long-time period of treatment ( 3 - 6 months) in low-dose ( 250 mg daily) doesn't change the lipid metabolism parameters in the blood of patients but their high antioxidant activity was observed. Really, after oxidation of probucol-contained LDL by C-15 animal lipoxygenase in these particles we identified the electron spin resonance signal of probucol phenoxyl radical that suggests the interaction of LDL-associated probucol with lipid radicals in vivo. We observed that 6 months treatment of patients with pravastatine (40 mg daily) or cerivastatin (0.4 mg daily) was followed by sufficiently accumulation of LDL lipoperoxides in vivo. In contrast, the 6 months therapy with pravastatin in combination with ubiquinone Q(10) ( 60 mg daily) sharply decreased the LDL initial lipoperoxides level whereas during treatment with cerivastatin in combination with probucol (250 mg daily) the LDL lipoperoxides concentration was maintained on an invariable level. Therefore, antioxidants may be very effective in the prevention of atherogenic oxidative modification of LDL during HMG-CoA reductase inhibitors therapy. [References: 43] LG - English PT - Article SB - Current Contents(R)/Life Sciences CC - Cell & Developmental Biology in Current Contents(R)/Life Sciences. EW - 2003 week 33 IN - Reprint available from: Lankin VZ Miasnikovs Inst Clin Cardioc, Cardiol Res Complex 3rd Cherepkovaskaya 15A Moscow 121552 Russia Miasnikovs Inst Clin Cardioc, Cardiol Res Complex Moscow 121552 Russia Inst Expt Cardiol Moscow Russia <8> UI - 702DA-0017 DD - ISI Document Solution: 702DA AU - Stafford GP AU - Scanlan J AU - McDonald IR AU - Murrell JC MA - cmurrell@bio.warwick.ac.uk RA - Murrell JC TI - rpoN, mmoR and mmoG, genes involved in regulating the expression of soluble methane monooxygenase in Methylosinus trichosporium OB3b SO - Microbiology. 149(Part 7):1771-1784, 2003 Jul. AS - Microbiology-(UK) 2003 Jul;149(Part 7):1771-1784 PU - SOC GENERAL MICROBIOLOGY, MARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLAND. URL: http://www.socgenmicrobiol.org.uk/default.htm IS - 1350-0872 MH - Methylococcus-capsulatus bath MH - Transcriptional regulation MH - Bradyrhizobium-japonicum MH - Rhodobacter-sphaeroides MH - Obligate methanotrophs MH - Alcaligenes-eutrophus MH - Sigma(54) sigma(n) MH - Molecular analysis MH - Nitrogen-fixation MH - Escherichia-coli. AB - The methanotrophic bacterium Methylosinus trichosporium OB3b converts methane to methanol using two distinct forms of methane monooxygenase (MMO) enzyme: a cytoplasmic soluble form (sMMO) and a membrane-bound form (pMMO). The transcription of these two. operons is known to proceed in a reciprocal fashion with sMMO expressed at low copper-to-biomass ratios and pMMO at high copper-to-biomass ratios. Transcription of the smmo operon is initiated from a sigma(N) promoter 5' of mmoX. In this study the genes encoding sigma(N) (rpoN) and a typical a sigma(N)-dependent transcriptional activator (mmoR) were cloned and sequenced. mmoR, a regulatory gene, and mmoG, a gene encoding a GroEL homologue, lie 5' of the structural genes for the sMMO enzyme. Subsequent mutation of rpoN and mmoR by marker-exchange mutagenesis resulted in strains Gm1 and JS1, which were unable to express functional sMMO or initiate transcription of mmoX. An rpoN mutant was also,unable to fix nitrogen or use nitrate as sole nitrogen source, indicating that sigma(N) plays a role in both nitrogen and carbon metabolism in Ms. trichosporium OB3b. The data also indicate that mmoG is transcribed in a or sigma(N)- and MmoR-independent manner. Marker-exchange mutagenesis of mmoG revealed that MmoG is necessary for smmo gene transcription and activity and may be an MmoR-specific chaperone required for functional assembly of transcriptionally competent MmoR in vivo. The data presented allow the proposal of a more complete model for copper-mediated regulation of smmo gene expression. [References: 58] LG - English PT - Article SB - Current Contents(R)/Life Sciences CC - Microbiology in Current Contents(R)/Life Sciences. EW - 2003 week 33 IN - Reprint available from: Murrell JC Univ Warwick, Dept Biol Sci Coventry CV4 7AL W Midlands England Univ Warwick, Dept Biol Sci Coventry CV4 7AL W Midlands England <9> UI - 701PN-0005 DD - ISI Document Solution: 701PN AU - Berthiaume F AU - MacDonald AD AU - Kang YH AU - Yarmush ML MA - ireis@sbi.org RA - Yarmush ML TI - Control analysis of mitochondrial metabolism in intact hepatocytes: effect of interleukin-1 beta and interleukin-6 SO - Metabolic Engineering. 5(2):108-123, 2003 Apr. AS - Metab. Eng 2003 Apr;5(2):108-123 PU - ACADEMIC PRESS INC ELSEVIER SCIENCE, 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA. URL: http://www.apnet.com IS - 1096-7176 MH - Liver MH - Collagen sandwich culture MH - Metabolic control analysis MH - Antimycin a MH - Oligomycin. MH - Tumor-necrosis-factor MH - Cultured rat hepatocytes MH - Nitric-oxide production MH - Acute-phase response MH - Factor-alpha MH - Oxidative-phosphorylation MH - Sandwich configuration MH - Atp turnover MH - Multidrug-resistance MH - Energy-metabolism. AB - Interleukin-1beta (IL-1beta) and interleukin-6 (IL-6) are produced by hepatic nonparenchymal cells after systemic injury and have been reported to inhibit ATP synthesis in hepatocytes, which may contribute to hepatic dysfunction in inflammatory states. To elucidate the mechanisms of action of IL-1beta and IL-6 on hepatocellular ATP synthesis, we measured the oxygen uptake rate (OUR) and mitochondrial membrane potential (NIMP) of stable hepatocyte cultures, and analyzed the dynamic MMP response following the addition of mitochondrial inhibitors (antimycin A and oligomycin) with a model of mitochondrial metabolism. IL-1beta reduced mitochondrial OUR coupled to ATP synthesis via inhibition of phosphorylation reactions which dissipate the MMP, including ATP synthesis and consumption. Furthermore, the ATP synthesis rate in cytokine-free and IL-1beta-treated hepatocytes was controlled primarily by phosphorylation reactions, which corresponds to a state where the ATP synthesis rate closely follows the cellular energy demand. Thus, IL-1beta-mediated effects on electron transport and substrate oxidation reactions are not likely to significantly impact on ATP synthesis. IL-6 did not reduce mitochondrial OUR coupled to ATP synthesis, but shifted the control for ATP synthesis towards processes which generate the MMP, indicating that IL-6 induces a metabolic state where cellular functions are limited by the mitochondrial energy supply. (C) 2003 Elsevier Science (USA). All rights reserved. [References: 71] LG - English PT - Article SB - Current Contents(R)/Agriculture, Biology & Environmental Sciences CC - Biotechnology & Applied Microbiology in Current Contents(R)/Agricultural, Biology & Environmental Sciences. EW - 2003 week 33 IN - Reprint available from: Yarmush ML Harvard Univ, Sch Med, Massachusetts Gen Hosp, Ctr Engn Med Surg Sci Boston, MA 02114 USA Harvard Univ, Sch Med, Massachusetts Gen Hosp, Ctr Engn Med Surg Sci Boston, MA 02114 USA Shriners Hosp Children Boston, MA 02114 USA <10> UI - 702FP-0022 DD - ISI Document Solution: 702FP AU - Neumann E AU - Moser R AU - Snyers L AU - Blaas D AU - Hewat EA MA - hewat@ibs.fr RA - Hewat EA TI - A cellular receptor of human rhinovirus type 2, the very-low-density lipoprotein receptor, binds to two neighboring proteins of the viral capsid SO - Journal of Virology. 77(15):8504-8511, 2003 Aug. AS - J. Virol 2003 Aug;77(15):8504-8511 PU - AMER SOC MICROBIOLOGY, 1752 N ST NW, WASHINGTON, DC 20036-2904 USA. URL: http://www.asmusa.org IS - 0022-538X MH - Common cold virus MH - Ldl receptor MH - Conformational-changes MH - Structural view MH - Hela-cells MH - Domain MH - Identification MH - Macromolecules MH - Components MH - Resolution. AB - The very-low-density lipoprotein receptor (VLDL-R) is a receptor for the minor-group human rhinoviruses (HRVs). Only two of the eight binding repeats of the VLDL-R bind to HRV2, and their footprints describe an annulus on the dome at each fivefold axis. By studying the complex formed between a selection of soluble fragments of the VLDL-R and HRV2, we demonstrate that it is the second and third repeats that bind. We also show that artificial concatemers of the same repeat can bind to HRV2 with the same footprint as that for the native receptor. In a 16-Angstrom-resolution cryoelectron microscopy map of HRV2 in complex with the VLDL-R, the individual repeats are defined. The third repeat is strongly bound to charged and polar residues of the H1 and BC loops of viral protein 1 (VP1), while the second repeat is more weakly bound to the neighboring VP1. The footprint of the strongly bound third repeat extends down the north side of the canyon. Since the receptor molecule can bind to two adjacent copies of VP1, we suggest that the bound receptor "staples" the VP1s together and must be detached before release of the RNA can occur. When the receptor is bound to neighboring sites on HRV2, steric hindrance prevents binding of the second repeat. [References: 36] LG - English PT - Article SB - Current Contents(R)/Life Sciences CC - Microbiology in Current Contents(R)/Life Sciences. EW - 2003 week 33 IN - Reprint available from: Hewat EA Inst Biol Struct Jean Pierre Ebel 41 Rue Jules Horowitz F-38027 Grenoble France Inst Biol Struct Jean Pierre Ebel F-38027 Grenoble France Univ Vienna, Inst Med Biochem, Vienna Bioctr A-1030 Vienna Austria <11> UI - 700YC-0024 DD - ISI Document Solution: 700YC AU - Ma DJ AU - Musto R AU - Smith KM AU - La Mar GN MA - lamar@indigo.ucdavis.edu RA - La Mar GN TI - Solution NMR characterization of the electronic structure and magnetic properties of high-spin ferrous heme in deoxy myoglobin from Aplysia limacina SO - Journal of the American Chemical Society. 125(28):8494-8504, 2003 Jul 16. AS - J. Am. Chem. Soc 2003 Jul 16;125(28):8494-8504 PU - AMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 USA. URL: http://pubs.acs.org IS - 0002-7863 MH - Overhauser effect measurements MH - Mitochondrial cytochrome-c MH - Hyperfine shift pattern MH - Ray crystal-structure MH - Rhodobacter-capsulatus MH - Active-site MH - Ferricytochrome-c MH - Solution h-1-nmr MH - Contact shifts MH - Rhodopseudomonas-palustris. AB - Solution H-1 NMR has been used to elucidate the magnetic properties and electronic structure of the prosthetic group in high-spin, ferrous deoxy myoglobin from the sea hare Aplysia limacina. A sufficient number of dipolar shifted residue signals were assigned to allow the robust determination of the orientation and anisotropy of the paramagnetic susceptibility tensor, X. The resulting quantitative description of dipolar shifts allows a determination of the contact shifts for the heme. X was found to be axial, with DeltaX(ax) = -2.07 x 10(-8) m(3)/mol, with the major axis tilted (similar to76degrees) almost into the heme plane and in the general direction of the orientation of the axial HisF8 imidazole plane which coincides approximately with the beta-,delta-meso axis. The factored contact shifts for the heme are shown to be consistent with the transfer of positive A spin density into one of the two components of the highest filled pi molecular orbital, 3e(pi), and the transfer of negative A-spin density, via spin-spin correlation, into the orthogonal excited-state component of the 3e(pi) molecular orbital. The thermal population of the excited state leads to strong deviation from the Curie law for the heme substituents experiencing primarily the negative A-spin density. The much larger transfer of negative spin density via the spin-paired dpi orbital into the excited state 3e(pi) in high-spin iron(II) than in low-spin iron(III) hemoproteins is attributed to the much stronger correlation exerted by the four unpaired spin on the iron in the former, as compared to the single unpaired spins on iron in the latter. [References: 74] LG - English PT - Article SB - Current Contents(R)/Life Sciences Current Contents(R)/Physical, Chemical & Earth Sciences CC - Chemistry & Analysis in Current Contents(R)/Life Sciences. Chemistry in Current Contents(R)/Physical, Chemical & Earth Sciences. EW - 2003 week 33 IN - Reprint available from: La Mar GN Univ Calif Davis, Dept Chem Davis, CA 95616 USA Univ Calif Davis, Dept Chem Davis, CA 95616 USA Univ Roma La Sapienza, Dept Biochem Sci Rome Italy <12> UI - 701DR-0006 DD - ISI Document Solution: 701DR AU - Haslam R AU - Borland A AU - Maxwell K AU - Griffiths H MA - richard.haslam@bbsrc.ac.uk RA - Haslam R TI - Physiological responses of the CAM epiphyte Tillandsia usneoides L. (Bromeliaceae) to variations in light and water supply SO - Journal of Plant Physiology. 160(6):627-634, 2003 Jun. AS - J. Plant Physiol 2003 Jun;160(6):627-634 PU - URBAN & FISCHER VERLAG, BRANCH OFFICE JENA, P O BOX 100537, D-07705 JENA, GERMANY. URL: http://www.urbanfischer.de IS - 0176-1617 MH - Bromeliaceae MH - Chlorophyll fluorescence MH - Crassulacean acid metabolism MH - Epiphyte MH - Pepc MH - Photosynthetic plasticity MH - Rubisco MH - Tillandsia usneoides. MH - Crassulacean acid metabolism MH - Plant kalanchoe-daigremontiana MH - Comparative ecophysiology MH - Guzmania-monostachia MH - C-3 bromeliads MH - Gas-exchange MH - Spanish moss MH - Co2 MH - Ecology MH - Photoinhibition. AB - In an effort to understand the mechanisms that sustain rootless atmospheric plants, the modulation of Crassulacean acid metabolism (CAM) in response to variations in irradiance and water supply was investigated in the epiphyte Tillandsia usneoides. Plants were acclimated to three light regimes, i.e. high, intermediate and low, with integrated photon flux densities (PFD) of 14.40, 8.64 and 4.32 mol m(-2) d(-1) equivalent to an instantaneous PFD of 200, 100, and 50 mumol m(-2) s(-1), respectively. Daily watering was then withdrawn from half of the plants at each PFD for 7d prior to sampling. In response to the three PFD treatments, chlorophyll content increased in plants acclimated to lower irradiances. Light response curves using non-invasive measurements of chlorophyll fluorescence demonstrated that photosystem II efficiency (PhiPSII) was maintained in high PFD acclimated plants, as they exhibited a larger capacity for non-photochemical dissipation (NPQ) of excess light energy than low PFD acclimated plants. Net CO2 uptake increased in response to higher PFD, reflecting enhanced carboxylation capacity in terms of phosphoenolpyruvate carboxylase (PEPc) and ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) activities. After water was withdrawn, nocturnal net CO2 uptake and accumulated levels of acidity declined in all PFD treatments, concomitant with increased respiratory recycling of malate. Examining the strategies employed by epiphytes such as T. usneodies to tolerate extreme light and water regimes has demonstrated the importance of physiological mechanisms that allow flexible carboxylation capacity and continued carbon cycling to maintain photosynthetic integrity. [References: 42] LG - English PT - Article SB - Current Contents(R)/Agriculture, Biology & Environmental Sciences Current Contents(R)/Life Sciences CC - Plant Sciences in Current Contents(R)/Agricultural, Biology & Environmental Sciences. Animal & Plant Sciences in Current Contents(R)/Life Sciences. EW - 2003 week 33 IN - Reprint available from: Haslam R Univ Newcastle, Sch Biol Newcastle Upon Tyne NE1 7RU Tyne & Wear England Univ Newcastle, Sch Biol Newcastle Upon Tyne NE1 7RU Tyne & Wear England Univ Cambridge, Dept Plant Sci Cambridge CB2 3EA England <13> UI - 701DR-0008 DD - ISI Document Solution: 701DR AU - Perez FJ AU - Maureira V MA - frperez@abello.uchile.cl RA - Perez FJ TI - Inactivation in vivo of basic peroxidase and increased content of H2O2 in grapevine leaves post treatment with DTT and Paraquat SO - Journal of Plant Physiology. 160(6):645-650, 2003 Jun. AS - J. Plant Physiol 2003 Jun;160(6):645-650 PU - URBAN & FISCHER VERLAG, BRANCH OFFICE JENA, P O BOX 100537, D-07705 JENA, GERMANY. URL: http://www.urbanfischer.de IS - 0176-1617 MH - Grapevine-h2o2 MH - Peroxidases MH - Paraquat MH - Dtt-oxidative damage. MH - Hydrogen-peroxide MH - Dehydroascorbate reductase MH - Ascorbate peroxidase MH - Photosystem-i MH - Chloroplasts MH - Photoinhibition MH - Spinach MH - Plant MH - Photosynthesis MH - Temperatures. AB - Substantial differences in the in vivo effect of paraquat (Pq) and IDTT on basic peroxidase (GBPx) activity and on H2O2 levels were found in grapevine leaves cv. Sultana. GBPx activity decreased and H2O2 levels increased in illuminated Pq treated leaf-discs. Inactivation of GBPx and accumulation of H2O2 depended on the duration and intensity of the illumination to which discs were exposed. Since GBPx was inactivated directly by H(2)o(2) and not by Pq in leaf extracts, and since GBPx are cytosolic isoenzymes and H2O2 is a stable molecule that can easily permeate chloroplast membranes, we concluded that Pq inactivation of GBPx in vivo is mediated by H2O2. In contrast to the effect induced by Pq, IDTT directly inactivated GBPx in leaf extracts. In leaf-discs, however, it reduced GBPx activity in the absence of light, although the levels of H2O2 increased only after exposure of the discs, to high irradiance, suggesting that under excess of light, a significant fraction of the photosynthetically produced electrons are dissipated through the water-water cycle and H2O2 accumulates as a consequence of GBPx inactivation. [References: 19] LG - English PT - Article SB - Current Contents(R)/Agriculture, Biology & Environmental Sciences Current Contents(R)/Life Sciences CC - Plant Sciences in Current Contents(R)/Agricultural, Biology & Environmental Sciences. Animal & Plant Sciences in Current Contents(R)/Life Sciences. EW - 2003 week 33 IN - Reprint available from: Perez FJ Univ Chile, Fac Ciencias, Lab Bioquim Vegetal Casilla 653 Santiago Chile Univ Chile, Fac Ciencias, Lab Bioquim Vegetal Santiago Chile <14> UI - 701DR-0010 DD - ISI Document Solution: 701DR AU - Monneveux P AU - Pastenes C AU - Reynolds MP MA - p.monneveux@cgiar.org RA - Monneveux P TI - Limitations to photosynthesis under light and heat stress in three high-yielding wheat genotypes SO - Journal of Plant Physiology. 160(6):657-666, 2003 Jun. AS - J. Plant Physiol 2003 Jun;160(6):657-666 PU - URBAN & FISCHER VERLAG, BRANCH OFFICE JENA, P O BOX 100537, D-07705 JENA, GERMANY. URL: http://www.urbanfischer.de IS - 0176-1617 MH - Carbon isotope discrimination MH - Chlorophyll fluorescence MH - Gas exchange MH - Photo-inhibition MH - Wheat (triticum aestivum l.). MH - Carbon-isotope discrimination MH - Chlorophyll fluorescence MH - Photosystem-ii MH - Gas-exchange MH - High-temperature MH - Use efficiency MH - Leaf structure MH - Photoinhibition MH - Productivity MH - Plants. AB - Three high-yielding wheat genotypes (T aestivum L., c.v. Siete Cerros, Seri and Bacanora, released in 1966, 1982 and 1988, respectively) were grown under irrigation in two high radiation, low relative humidity environments (Tlaltizapan and Ciudad Obregon CIMMYT experimental stations, Mexico). Gas exchange and fluorescence parameters were assessed on the flag leaf during the day. Carbon isotope discrimination (Delta) was analysed in flag leaf at anthesis and in grain at maturity. In both environments, gas exchange and fluorescence parameters varied markedly with irradiance and temperature. Analysis of their respective variation indicated the occurrence of photo-respiration and photo-inhibition, particularly in Tlaltizapan, the warmest environment, and in Siete Cerros. In Ciudad Obregon (high-yielding environment) lower C-i (internal CO2 concentration) and DeltaL(a) (carbon isotope discrimination of the leaf) suggested a higher intrinsic photosynthetic capacity in the variety Bacanora. Higher yield of this genotype was also associated with higher Fv'/Fo' (ratio of photochemical and non photochemical rate constants in the light) and Fm'/Fm (ratio of the non photochemical rate constants in the dark and light adapted state). [References: 36] LG - English PT - Article SB - Current Contents(R)/Agriculture, Biology & Environmental Sciences Current Contents(R)/Life Sciences CC - Plant Sciences in Current Contents(R)/Agricultural, Biology & Environmental Sciences. Animal & Plant Sciences in Current Contents(R)/Life Sciences. EW - 2003 week 33 IN - Reprint available from: Monneveux P CIMMYT, INRA CIMMYT Wheat Project AP 6-641 Mexico City 06600 DF Mexico CIMMYT, INRA CIMMYT Wheat Project Mexico City 06600 DF Mexico Univ Chile, Fac Ciencias Agron, Lab Fisiol Estres Vegetal Santiago 1004 Chile CIMMYT, Wheat Program Mexico City 06600 DF Mexico <15> UI - 701ER-0059 DD - ISI Document Solution: 701ER AU - Hemmerlin A AU - Hoeffler JF AU - Meyer O AU - Tritsch D AU - Kagan IA AU - Grosdemange-Billiard C AU - Rohmer M AU - Bach TJ MA - Thomas.Bach@bota-ulp.u-strasbg.fr RA - Bach TJ TI - Cross-talk between the cytosolic mevalonate and the plastidial methylerythritol phosphate pathways in Tobacco Bright Yellow-2 cells SO - Journal of Biological Chemistry. 278(29):26666-26676, 2003 Jul 18. AS - J. Biol. Chem 2003 Jul 18;278(29):26666-26676 PU - AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA. URL: http://www.asbmb.org IS - 0021-9258 MH - Coenzyme-a reductase MH - 1-deoxy-d-xylulose 5-phosphate reductoisomerase MH - Hmg-coa reductase MH - Isoprenoid biosynthesis MH - Isopentenyl diphosphate MH - Terpenoid biosynthesis MH - Escherichia-coli MH - Radish seedlings MH - Independent biosynthesis MH - Competitive inhibitor. AB - In plants, two pathways are utilized for the synthesis of isopentenyl diphosphate, the universal precursor for isoprenoid biosynthesis. The key enzyme of the cytoplasmic mevalonic acid (MVA) pathway is 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR). Treatment of Tobacco Bright Yellow-2 (TBY-2) cells by the HMGR-specific inhibitor mevinolin led to growth reduction and induction of apparent HMGR activity, in parallel to an increase in protein representing two HMGR isozymes. Maximum induction was observed at 24 h. 1-Deoxy-D-xylulose (DX), the dephosphorylated first precursor of the plastidial 2-C-methyl-D-erythritol4-phosphate ( MEP) pathway, complemented growth inhibition by mevinolin in the low millimolar concentration range. Furthermore, DX partially re-established feedback repression of mevinolin-induced HMGR activity. Incorporation studies with [1,1,1,4-H-2(4)]DX showed that sterols, normally derived from MVA, in the presence of mevinolin are synthesized via the MEP pathway. Fosmidomycin, an inhibitor of 1-deoxy-D-xylulose-5-phosphate reductoisomerase, the second enzyme of the MEP pathway, was utilized to study the reverse complementation. Growth inhibition by fosmidomycin of TBY-2 cells could be partially overcome by MVA. Chemical complementation was further substantiated by incorporation of [2-C-13] MVA into plastoquinone, representative of plastidial isoprenoids. Best rates of incorporation of exogenous stably labeled precursors were observed in the presence of both inhibitors, thereby avoiding internal isotope dilution. [References: 69] LG - English PT - Article SB - Current Contents(R)/Life Sciences CC - Biochemistry & Biophysics in Current Contents(R)/Life Sciences. EW - 2003 week 33 IN - Reprint available from: Bach TJ CNRS, UPR 2357, Inst Biol Mol Plantes 28 Rue Goethe F-67038 Strasbourg France CNRS, UPR 2357, Inst Biol Mol Plantes F-67038 Strasbourg France Univ Strasbourg 1, CNRS, Inst Le Bel F-67070 Strasbourg France <16> UI - 701ER-0060 DD - ISI Document Solution: 701ER AU - Steuber J MA - fritz-steuber@micro.biol.ethz.ch RA - Steuber J TI - The C-terminally truncated NuoL subunit (ND5 homologue) of the Na+-dependent complex I from Escherichia coli transports Na+ SO - Journal of Biological Chemistry. 278(29):26817-26822, 2003 Jul 18. AS - J. Biol. Chem 2003 Jul 18;278(29):26817-26822 PU - AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA. URL: http://www.asbmb.org IS - 0021-9258 MH - Nadh-ubiquinone oxidoreductase MH - Bovine heart-mitochondria MH - Quinone oxidoreductase MH - Binding-sites MH - Klebsiella-pneumoniae MH - Translocating nadh MH - Antiporter MH - Membrane MH - Growth MH - Overproduction. AB - The NADH: quinone oxidoreductase ( complex I) from Escherichia coli acts as a primary Na+ pump. Expression of a C-terminally truncated version of the hydrophobic NuoL subunit (ND5 homologue) from E. coli complex I resulted in Na+-dependent growth inhibition of the E. coli host cells. Membrane vesicles containing the truncated NuoL subunit ( NuoL(N)) exhibited 2-4-fold higher Na+ uptake activity than control vesicles without NuoL(N). Respiratory proton transport into inverted vesicles containing NuoL(N) decreased upon addition of Na+, but was not affected by K+, indicating a Na+-dependent increase of proton permeability of membranes in the presence of NuoL(N). The His-tagged NuoL(N) protein was solubilized, enriched by affinity chromatography, and reconstituted into proteoliposomes. Reconstituted His(6)-NuoL(N) facilitated the uptake of Na+ into the proteoliposomes along a concentration gradient. This Na+ uptake was prevented by EIPA (5-(N-ethyl-N-isopropyl)amiloride), which acts as inhibitor against Na+/H+ antiporters. [References: 47] LG - English PT - Article SB - Current Contents(R)/Life Sciences CC - Biochemistry & Biophysics in Current Contents(R)/Life Sciences. EW - 2003 week 33 IN - Reprint available from: Steuber J ETH, Inst Mikrobiol, ETH Zentrum Schmelzbergstr 7 CH-8092 Zurich Switzerland ETH, Inst Mikrobiol, ETH Zentrum CH-8092 Zurich Switzerland <17> UI - 701ER-0061 DD - ISI Document Solution: 701ER AU - Stalz WD AU - Greie JC AU - Deckers-Hebestreit G AU - Altendorf K MA - deckers-hebestreit@biologie.uni-osnabrueck.de RA - Deckers-Hebestreit G TI - Direct interaction of subunits a and b of the F-0 complex of Escherichia coli ATP synthase by forming an ab(2) subcomplex SO - Journal of Biological Chemistry. 278(29):27068-27071, 2003 Jul 18. AS - J. Biol. Chem 2003 Jul 18;278(29):27068-27071 PU - AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA. URL: http://www.asbmb.org IS - 0021-9258 MH - Proton-translocating atpase MH - Cross-linking MH - Adenosine-triphosphatase MH - Membrane moiety MH - Rotary motor MH - C oligomer MH - F1f0 MH - Reconstitution MH - Organization MH - Mechanism. AB - The addition of a His(6) tag to the N terminus of subunit a of the F-0 complex of the Escherichia coli ATP synthase allowed the purification of an ab(2) subcomplex after solubilization of membranes with n-dodecyl-beta-D-maltoside and subsequent nickel-nitrilotriacetic acid affinity chromatography. After co-reconstitution of the ab(2) subcomplex with purified subunit c, passive proton translocation rates as well as coupled ATPase activities after binding of F-1 were measured that were comparable with those of wild type F-0. The interaction between subunits a and b, which has been shown to be stoichiometric and functional, is not triggered by any cross-linking reagent and therefore reflects subunit interactions occurring within the F-0 complex in vivo. [References: 34] LG - English PT - Article SB - Current Contents(R)/Life Sciences CC - Biochemistry & Biophysics in Current Contents(R)/Life Sciences. EW - 2003 week 33 IN - Reprint available from: Deckers-Hebestreit G Univ Osnabruck, Fachbereich Biol Chem, Abt Mikrobiol D-49069 Osnabruck Germany Univ Osnabruck, Fachbereich Biol Chem, Abt Mikrobiol D-49069 Osnabruck Germany <18> UI - 701ER-0117 DD - ISI Document Solution: 701ER AU - Gonzalez-Gronow M AU - Kalfa T AU - Johnson CE AU - Gawdi G AU - Pizzo SV MA - gonza002@mc.duke.edu RA - Gonzalez-Gronow M TI - The voltage-dependent anion channel is a receptor for plasminogen kringle 5 on human endothelial cells SO - Journal of Biological Chemistry. 278(29):27312-27318, 2003 Jul 18. AS - J. Biol. Chem 2003 Jul 18;278(29):27312-27318 PU - AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA. URL: http://www.asbmb.org IS - 0021-9258 MH - Tumor-growth invivo MH - Angiogenesis inhibitor MH - Plasma-membrane MH - Down-regulation MH - Caveolae MH - Proteins MH - Binding MH - Angiostatin MH - Streptokinase MH - Expression. AB - Human plasminogen contains structural domains that are termed kringles. Proteolytic cleavage of plasminogen yields kringles 1 - 3 or 4 and kringle 5 (K5), which regulate endothelial cell proliferation. The receptor for kringles 1 - 3 or 4 has been identified as cell surface-associated ATP synthase; however, the receptor for K5 is not known. Sequence homology exists between the plasminogen activator streptokinase and the human voltage-dependent anion channel ( VDAC); however, a functional relationship between these proteins has not been reported. A streptokinase binding site for K5 is located between residues Tyr(252)-Lys(283), which is homologous to the primary sequence of VDAC residues Tyr(224)-Lys(255). Antibodies against these sequences react with VDAC and detect this protein on the plasma membrane of human endothelial cells. K5 binds with high affinity (K-d of 28 nM) to endothelial cells, and binding is inhibited by these antibodies. Purified VDAC binds to K5 but only when reconstituted into liposomes. K5 also interferes with mechanisms controlling the regulation of intracellular Ca2+ via its interaction with VDAC. K5 binding to endothelial cells also induces a decrease in intracellular pH and hyperpolarization of the mitochondrial membrane. These studies suggest that VDAC is a receptor for K5. [References: 56] LG - English PT - Article SB - Current Contents(R)/Life Sciences CC - Biochemistry & Biophysics in Current Contents(R)/Life Sciences. EW - 2003 week 33 IN - Reprint available from: Gonzalez-Gronow M Duke Univ, Med Ctr, Dept Pathol Box 3712 DUMC Durham, NC 27710 USA Duke Univ, Med Ctr, Dept Pathol Durham, NC 27710 USA Duke Univ, Med Ctr, Dept Pediat Durham, NC 27710 USA <19> UI - 701ER-0124 DD - ISI Document Solution: 701ER AU - Wang WG AU - Yang XL AU - de Silanes IL AU - Carling D AU - Gorospe M MA - myriam-gorospe@nih.gov RA - Gorospe M TI - Increased AMP : ATP ratio and AMP-activated protein kinase activity during cellular senescence linked to reduced HuR function SO - Journal of Biological Chemistry. 278(29):27016-27023, 2003 Jul 18. AS - J. Biol. Chem 2003 Jul 18;278(29):27016-27023 PU - AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA. URL: http://www.asbmb.org IS - 0021-9258 MH - Au-rich element MH - Rna-binding protein MH - Messenger-rna MH - Signaling pathways MH - Oxidative stress MH - Elav protein MH - In-vivo MH - Transcription MH - Degradation MH - Expression. AB - Cytoplasmic export of the RNA-binding protein HuR, a process that critically regulates its function, was recently shown to be inhibited by the AMP-activated protein kinase ( AMPK). In the present investigation, treatment of human fibroblasts with AMPK activators such as 5-amino-imidazole-4-carboxamide riboside, antimycin A, and sodium azide inhibited cell growth and lowered the expression of proliferative genes. As anticipated, AMPK activation also decreased both the cytoplasmic HuR levels and the association of HuR with target radiolabeled transcripts encoding such proliferative genes. HuR function was previously shown to be implicated in the maintenance of a "young cell" phenotype in models of replicative cellular senescence. We therefore postulated that AMPK activation in human fibroblasts might contribute to the implementation of the senescence phenotype through mechanisms that included a reduction in HuR cytoplasmic presence. Indeed, AMP: ATP ratios were 2 -3-fold higher in senescent fibroblasts compared with young fibroblasts. Accordingly, in vitro senescence was accompanied by a marked elevation in AMPK activity. Evidence that increased AMPK activity directly contributed to the implementation of the senescent phenotype was obtained through two experimental approaches. First, use of AMPK activators triggered senescence characteristics in fibroblasts, such as the acquisition of senescence-associated beta-galactosidase (beta-gal) activity and increased p16(INK4a) expression. Second, infection of cells with an adenoviral vector that expresses active AMPK increased senescence-associated beta-gal activity, whereas infection with an adenovirus that expresses dominant-negative AMPK decreased senescence-associated beta-gal activity. Together, our results indicate that AMPK activation can cause premature fibroblast senescence through mechanisms that likely involve reduced HuR function. [References: 44] LG - English PT - Article SB - Current Contents(R)/Life Sciences CC - Biochemistry & Biophysics in Current Contents(R)/Life Sciences. EW - 2003 week 33 IN - Reprint available from: Gorospe M NIA, LCMB, IRP, NIH 5600 Nathan Shock Dr Baltimore, MD 21224 USA NIA, LCMB, IRP, NIH Baltimore, MD 21224 USA Imperial Coll Sch Med, MRC, Ctr Clin Sci London W12 0NN England <20> UI - 702UL-0058 DD - ISI Document Solution: 702UL AU - Xu W AU - Chitnis P AU - Valieva A AU - van der Est A AU - Pushkar YN AU - Krzystyniak M AU - Teutloff C AU - Zech SG AU - Bittl R AU - Stehlik D AU - Zybailov B AU - Shen GZ AU - Golbeck JH MA - JHG5@psu.edu RA - Golbeck JH TI - Electron transfer in cyanobacterial photosystem I - I. Physiological and spectroscopic characterization of site-directed mutants in a putative electron transfer pathway from A(0) through A(1) to F-x SO - Journal of Biological Chemistry. 278(30):27864-27875, 2003 Jul 25. AS - J. Biol. Chem 2003 Jul 25;278(30):27864-27875 PU - AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA. URL: http://www.asbmb.org IS - 0021-9258 MH - Paramagnetic-resonance spectroscopy MH - Photosynthetic reaction centers/ MH - Structural model MH - Acceptor a(1) MH - Resolution MH - Bacteria MH - Binding MH - Endor. AB - The Photosystem I (PS I) reaction center contains two branches of nearly symmetric cofactors bound to the PsaA and PsaB heterodimer. From the x-ray crystal structure it is known that Trp(PsaA)(697) and Trp(PsaB)(677) are pi-stacked with the head group of the phylloquinones and are H-bonded to Ser(692) PsaA and Ser(672) PsaB, whereas Arg(694) (PsaA) and Arg(PsaB)(674) are involved in a H-bonded network of side groups that connects the binding environments of the phylloquinones and F-X. The mutants W697F(PsaA), W677F(PsaB), S692C(PsaA), S672C(PsaB), R694A(PsaA), and R674A(PsaB) were constructed and characterized. All mutants grew photoautotrophically, yet all showed diminished growth rates compared with the wild-type, especially at higher light intensities. EPR and electron nuclear double resonance (ENDOR) studies at both room temperature and in frozen solution showed that the PsaB mutants were virtually identical to the wildtype, whereas significant effects were observed in the PsaA mutants. Spin polarized transient EPR spectra of the P-700 (+)A(1)(-) radical pair show that none of the mutations causes a significant change in the orientation of the measured phylloquinone. Pulsed ENDOR spectra reveal that the W697F(PsaA) mutation leads to about a 5% increase in the hyperfine coupling of the methyl group on the phylloquinone ring, whereas the S692C(PsaA) mutation causes a similar decrease in this coupling. The changes in the methyl hyperfine coupling are also reflected in the transient EPR spectra of P-700 (+)A(1)(-) and the CW EPR spectra of photoaccumulated A(1)(-). We conclude that: (i) the transient EPR spectra at room temperature are predominantly from radical pairs in the PsaA branch of cofactors; (ii) at low temperature the electron cycle involving P-700 and A(1) similarly occurs along the PsaA branch of cofactors; and (iii) mutation of amino acids in close contact with the PsaA side quinone leads to changes in the spin density distribution of the reduced quinone observed by EPR. [References: 24] LG - English PT - Article SB - Current Contents(R)/Life Sciences CC - Biochemistry & Biophysics in Current Contents(R)/Life Sciences. EW - 2003 week 33 IN - Reprint available from: Golbeck JH Penn State Univ, Dept Biochem & Mol Biol University Pk, PA 16802 USA Penn State Univ, Dept Biochem & Mol Biol University Pk, PA 16802 USA Iowa State Univ, Dept Biochem Biophys & Mol Biol Ames, IA 50011 USA Brock Univ, Dept Chem St Catharines ON L2S 3A1 Canada Free Univ Berlin, Fachbereich Phys D-14195 Berlin Germany <21> UI - 702UL-0059 DD - ISI Document Solution: 702UL AU - Xu W AU - Chitnis PR AU - Valieva A AU - van der Est A AU - Brettel K AU - Guergova-Kuras M AU - Pushkar YN AU - Zech SG AU - Stehlik D AU - Shen GZ AU - Zybailov B AU - Golbeck JH MA - JHG5@psu.edu RA - Golbeck JH TI - Electron transfer in cyanobacterial photosystem I - II. Determination of forward electron transfer rates of site-directed mutants in a putative electron transfer pathway from A(0) through A(1) to F-x SO - Journal of Biological Chemistry. 278(30):27876-27887, 2003 Jul 25. AS - J. Biol. Chem 2003 Jul 25;278(30):27876-27887 PU - AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA. URL: http://www.asbmb.org IS - 0021-9258 MH - Photosynthetic reaction centers MH - Bacterial reaction centers MH - Correlated radical pairs MH - Iron-sulfur centers MH - Transient epr MH - Spin polarization MH - Quantum beats MH - 3-dimensional structure MH - Temperature-dependence MH - Angstrom resolution. AB - The directionality of electron transfer in Photosystem I (PS I) is investigated using site-directed mutations in the phylloquinone (Q(K)) and F-X binding regions of Synnechocystis sp. PCC 6803. The kinetics of forward electron transfer from the secondary acceptor A(1) (phylloquinone) were measured in mutants using time-resolved optical difference spectroscopy and transient EPR spectroscopy. In whole cells and PS I complexes of the wild-type both techniques reveal a major, slow kinetic component of tau approximate to 300 ns while optical data resolve an additional minor kinetic component of tau approximate to 10 ns. Whole cells and PS I complexes from the W697F(PsaA) and S692C(PsaA) mutants show a significant slowing of the slow kinetic component, whereas the W677F(PsaB) and S672C(PsaB) mutants show a less significant slowing of the fast kinetic component. Transient EPR measurements at 260 K show that the slow phase is similar to3 times slower than at room temperature. Simulations of the early time behavior of the spin polarization pattern of P-700 (+)A(1)(-), in which the decay rate of the pattern is assumed to be negligibly small, reproduce the observed EPR spectra at 260 K during the first 100 ns following laser excitation. Thus any spin polarization from P-700 F-+(X)- in this time window is very weak. From this it is concluded that the relative amplitude of the fast phase is negligible at 260 K or its rate is much less temperature-dependent than that of the slow component. Together, the results demonstrate that the slow kinetic phase results from electron transfer from Q(K)-A to F-X and that this accounts for at least 70% of the electrons. Although the assignment of the fast kinetic phase remains uncertain, it is not strongly temperature dependent and it represents a minor fraction of the electrons being transferred. All of the results point toward asymmetry in electron transfer, and indicate that forward transfer in cyanobacterial PS I is predominantly along the PsaA branch. [References: 35] LG - English PT - Article SB - Current Contents(R)/Life Sciences CC - Biochemistry & Biophysics in Current Contents(R)/Life Sciences. EW - 2003 week 33 IN - Reprint available from: Golbeck JH Penn State Univ, Dept Biochem & Mol Biol University Pk, PA 16802 USA Penn State Univ, Dept Biochem & Mol Biol University Pk, PA 16802 USA Iowa State Univ, Dept Biochem Biophys & Mol Biol Ames, IA 50011 USA Brock Univ, Dept Chem St Catharines ON L2S 3A1 Canada CEA Saclay, CNRS, URA 2096 F-91191 Gif Sur Yvette France CEA, Serv Bioenerget F-91191 Gif Sur Yvette France Inst Biol Physicochim, CNRS, UPR 1261 F-75005 Paris France Free Univ Berlin, Fachbereich Phys D-14195 Berlin Germany <22> UI - 702UL-0062 DD - ISI Document Solution: 702UL AU - Papucci L AU - Schiavone N AU - Witort E AU - Donnini M AU - Lapucci A AU - Tempestini A AU - Formigli L AU - Zecchi-Orlandini S AU - Orlandini G AU - Carella G AU - Brancato R AU - Capaccioli S MA - sergio@unifi.it RA - Capaccioli S TI - Coenzyme Q10 prevents apoptosis by inhibiting mitochondrial depolarization independently of its free radical scavenging property SO - Journal of Biological Chemistry. 278(30):28220-28228, 2003 Jul 25. AS - J. Biol. Chem 2003 Jul 25;278(30):28220-28228 PU - AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA. URL: http://www.asbmb.org IS - 0021-9258 MH - Permeability transition pore MH - Respiratory-chain complex MH - Activated protein-kinase MH - Programmed cell-death MH - Ubiquinone analogs MH - Mediated apoptosis MH - Hydrogen-peroxide MH - Oxidative stress MH - Plasma-membrane MH - Cytochrome-c. AB - The permeability transition pore (PTP) is a mitochondrial channel whose opening causes the mitochondrial membrane potential (Deltapsi) collapse that leads to apoptosis. Some ubiquinone analogues have been demonstrated previously to modulate the PTP open-closed transition in isolated mitochondria and thought to act through a common PTP-binding site rather than through oxidation-reduction reactions. We have demonstrated recently both in vitro and in vivo that the ubiquitous free radical scavenger and respiratory chain coenzyme Q(10) (CoQ(10)) prevents keratocyte apoptosis induced by excimer laser irradiation more efficiently than other antioxidants. On this basis, we hypothesized that the antiapoptotic property of CoQ(10) could be independent of its free radical scavenging ability and related to direct inhibition of PTP opening. In this study, we have verified this hypothesis by evaluating the antiapoptotic effects of CoQ(10) in response to apoptotic stimuli, serum starvation, antimycin A, and ceramide, which do not generate free radicals, in comparison to control, free radical-generating UVC irradiation. As hypothesized, CoQ(10) dramatically reduced apoptotic cell death, attenuated ATP decrease, and hindered DNA fragmentation elicited by all apoptotic stimuli. This was accompanied by inhibition of mitochondrial depolarization, cytochrome c release, and caspase 9 activation. Because these events are consequent to mitochondrial PTP opening, we suggest that the antiapoptotic activity of CoQ(10) could be related to its ability to prevent this phenomenon. [References: 63] LG - English PT - Article SB - Current Contents(R)/Life Sciences CC - Biochemistry & Biophysics in Current Contents(R)/Life Sciences. EW - 2003 week 33 IN - Reprint available from: Capaccioli S Univ Florence, Dept Expt Pathol & Oncol Viale GB Morgagni 50 I-50134 Florence Italy Univ Florence, Dept Expt Pathol & Oncol I-50134 Florence Italy Univ Florence, Dept Anat Histol & Forens Med I-50134 Florence Italy Univ Milan, Hosp San Raffaele, Dept Ophthalmol & Visual Sci I-20132 Milan Italy <23> UI - 702EQ-0002 DD - ISI Document Solution: 702EQ AU - Prakash JSS AU - Srivastava A AU - Strasser RJ AU - Mohanty P MA - Prasanna37@hotmail.com RA - Mohanty P TI - Senescence-induced alterations in the photosystem II functions of Cucumis sativus cotyledons: Probing of senescence driven alterations of photosystem II by chlorophyll alpha fluorescence induction O-J-I-P transients SO - Indian Journal of Biochemistry & Biophysics. 40(3):160-168, 2003 Jun. AS - Indian J. Biochem. Biophys 2003 Jun;40(3):160-168 PU - NATL INST SCIENCE COMMUNICATION, DR K S KRISHNAN MARG, NEW DELHI 110 012, INDIA IS - 0301-1208 MH - Chi a fluorescence MH - Cotyledons MH - Cucumis sativus MH - Senescence MH - Jip-test MH - Photosystem ii MH - O-j-i-p transients. MH - Photosynthetic electron-transport MH - Phaseolus-vulgaris l MH - A fluorescence MH - Rice seedlings MH - Primary leaves MH - Thylakoid membranes MH - Leaf senescence MH - Barley leaves MH - Chloroplasts MH - Temperature. AB - Senescence-induced alterations in photosystem II (PS II) structure-and photofunctions were probed in cucumber (Cucumis sativus) cotyledons, using fast O-J-I-P Chlorophyll a (Chl a) fluorescence transients. Analysis of measured and derived parameters of the fast, fluorescence O-J-I-P transient revealed senescence-induced alterations in (i), PS II acceptor side electron transfer equilibrium between Q(A) and Q(B), the primary stable and secondary acceptors of PS II; (ii), intersystem PQ pool size and (iii), affected electron transfer from PS II to PS I. Also, senescence of cotyledons triggered conversion of Q(A)-reducing (fully active) to non-Q(A)-reducing PS II (heat sink) centres. Further, some of the remaining active PS II centres showed a high apparent trapping efficiency due to clustering and energetic, connectivity (grouping) between the antennae of active and inactive centers. The overall density of active PS II reaction centers showed a temporal decrease due to the on set of foliar senescence. Thus, the fast Chl a fluorescence transients, with a time resolution of at least 50 mus and use of the equations of JIP-test, provide a valuable, non-invasive rapid biophysical probe to study the ageing in plants in terms of detecting photosynthetic activities and the heterogeneity of different types of photosynthetic units. Further, these results were found to be in agreement with the earlier in vitro studies using thylakoids isolated from senescing cotyledons where it was shown that senescence induced heterogeneity in PS ii centers affected Acceptor side Q(A)<---->Q(B) equilibrium. [References: 40] LG - English PT - Article SB - Current Contents(R)/Life Sciences CC - Biochemistry & Biophysics in Current Contents(R)/Life Sciences. EW - 2003 week 33 IN - Reprint available from: Mohanty P Reg Platn Resource Ctr Nayapalli Bhubaneswar 751015 Orissa India Jawaharlal Nehru Univ, Sch Life Sci New Delhi 110067 India Univ Rochester, Sch Med, Dept Biophys & Biochem Rochester, NY 14642 USA Univ Geneva, Bioenerget Lab CH-1254 Geneva Switzerland <24> UI - 703FZ-0003 DD - ISI Document Solution: 703FZ AU - Haut S AU - Brivet M AU - Touati G AU - Rustin P AU - Lebon S AU - Garcia-Cazorla A AU - Saudubray JM AU - Boutron A AU - Legrand A AU - Slama A MA - abdel.slama@bct.ap-hop-paris.fr RA - Slama A TI - A deletion in the human QP-C gene causes a complex III deficiency resulting in hypoglycaemia and lactic acidosis SO - Human Genetics. 113(2):118-122, 2003 Jul. AS - Hum. Genet 2003 Jul;113(2):118-122 PU - SPRINGER-VERLAG, 175 FIFTH AVE, NEW YORK, NY 10010 USA. URL: http://www.springer-ny.com IS - 0340-6717 MH - Mitochondrial cytochrome-b MH - Ubiquinone-binding protein MH - Respiratory-chain MH - Exercise intolerance MH - Oxidase deficiency MH - Point mutation MH - Cardiomyopathy MH - Reductase MH - Dna MH - Nomenclature. AB - Mitochondrial respiratory chain complex III (ubiquinol-cytochrome c reductase) consists of 11 subunits, only one (cytochrome b) being encoded by the mitochondrial DNA. Disorders of complex III are comparatively rare but are nevertheless present as a clinically heterogeneous group of diseases. To date, no mutation in any of the nuclear-encoded subunits has been described. We report here a deletion in the nuclear gene UQCRB encoding the human ubiquinone-binding protein of complex III (QP-C subunit or subunit VII) in a consanguineous family with an isolated complex III defect. In the proband, a homozygous 4-bp deletion was identified at nucleotides 338-341 of the cDNA predicting both a change in the last seven amino acids and an addition of a stretch of 14 amino acids at the C-terminal end of the protein. Both parents were found to be heterozygous for the deletion, which was absent from 55 controls. Low temperature (-196degreesC) spectral studies performed on isolated mitochondria from cultured skin fibroblast of the proband showed a decreased cytochrome b content suggestive of a role for the QP-C subunit in the assembly or maintenance of complex III structure. [References: 31] LG - English PT - Article SB - Current Contents(R)/Life Sciences CC - Molecular Biology & Genetics in Current Contents(R)/Life Sciences. EW - 2003 week 33 IN - Reprint available from: Slama A Hop Bicetre, AP HP, Lab Biochim 1 78 Rue Gen Leclerc F-94275 Le Kremlin Bicetre France Hop Bicetre, AP HP, Lab Biochim 1 F-94275 Le Kremlin Bicetre France Hop Necker Enfants Malad, Serv Pediat F-5743 Paris France Hop Necker Enfants Malad, INSERM, U393, Unite Rech Handicaps Genet Enfant F-5743 Paris France <25> UI - 703NG-0009 DD - ISI Document Solution: 703NG AU - Choy KJ AU - Deng YM AU - Hou JY AU - Wu B AU - Lau A AU - Witting PK AU - Stocker R MA - r.stocker@unsw.edu.au RA - Stocker R TI - Coenzyme Q(10) supplementation inhibits aortic lipid oxidation but fails to attenuate intimal thickening in balloon-injured New Zealand white rabbits SO - Free Radical Biology & Medicine. 35(3):300-309, 2003 Aug 1. AS - Free Radic. Biol. Med 2003 Aug 1;35(3):300-309 PU - PERGAMON-ELSEVIER SCIENCE LTD, THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND. URL: http://www.elsevier.nl IS - 0891-5849 MH - Antioxidant MH - Atherogenesis MH - Balloon angioplasty MH - Lipid peroxidation MH - Ubiquinol-10 MH - Ubiquinone-10 MH - Free radicals. MH - Low-density-lipoprotein MH - Tocopherol-mediated peroxidation MH - Human atherosclerotic lesions MH - Gene knockout mice MH - Alpha-tocopherol MH - Apolipoprotein-e MH - Vitamin-e MH - Oxidized lipids MH - Antioxidant bo-653 MH - Reductase-activity. AB - Oxidized lipoproteins are implicated in atherosclerosis, and some antioxidants attenuate the disease in animals. Coenzyme Q(10) (CoQ(10)) in its reduced form, ubiquinol-10, effectively inhibits lipoprotein oxidation in vitro and in vivo; CoQ(10) supplements also inhibit atherosclerosis in apolipoprotein E gene knockout (apoE-/-) mice. Here we tested the effect of dietary CoQ(10) supplements on intimal proliferation and lipoprotein lipid oxidation in balloon-injured, hypercholesterolemic rabbits. Compared to nonsupplemented chow, CoQ(10) supplementation (0.5% and 1.0%, w/w) significantly increased the plasma concentration of CoQ(10) and the resistance of plasma lipids to ex vivo oxidation. CoQ(10) supplements also increased the content of CoQ(10) in the aorta and liver, but not in the brain, skeletal muscle, kidney, and heart. Surprisingly, CoQ(10) supplementation at 1% increased the aortic concentrations of all lipids, particularly triacylglycerols, although it significantly inhibited the proportion of triacylglycerols present as hydroperoxides by > 80%. The observed increase in vessel wall lipid content was reflected in elevated plasma concentrations of cholesterol, cholesteryl esters and triacylglycerols, and hepatic levels of mRNA for 3-hydroxy-3-methylglutarylcoenzyme A reductase. CoQ(10) supplements did not attenuate lesion formation, assessed by the intima-to-media ratio of injured aortic vessels. Thus, like in apoE-/- mice, a high dose of supplemented CoQ(10) inhibits lipid oxidation in the artery wall of balloon-injured, hypercholesterolemic rabbits. However, unlike its antiatherosclerosis activity in the mice, CoQ(10) does not inhibit intimal hyperplasia in rabbits, thereby dissociating this disease process from lipid oxidation in the vessel wall. (C) 2003 Elsevier Inc. [References: 54] LG - English PT - Article SB - Current Contents(R)/Life Sciences CC - Biochemistry & Biophysics in Current Contents(R)/Life Sciences. EW - 2003 week 33 IN - Reprint available from: Stocker R Univ New S Wales, Sch Med Sci, Ctr Vasc Res Sydney NSW 2052 Australia Univ New S Wales, Sch Med Sci, Ctr Vasc Res Sydney NSW 2052 Australia Heart Res Inst, Biochem Grp Camperdown NSW Australia <26> UI - 700VF-0011 DD - ISI Document Solution: 700VF AU - Kang SW AU - Seo SK AU - Hill JM AU - Kwon B AU - Lee HW AU - Cho HR AU - Vinay DS AU - Kwon BS MA - bskwon@mail.ulsan.ac.kr RA - Kwon BS TI - Changes in gene expression in latent HSV-1-infected rabbit trigeminal ganglia following epinephrine iontophoresis SO - Current Eye Research. 26(3-4):225-229, 2003 Mar-Apr. AS - Curr. Eye Res 2003 Mar-Apr;26(3-4):225-229 PU - SWETS ZEITLINGER PUBLISHERS, P O BOX 825, 2160 SZ LISSE, NETHERLANDS. URL: http://www.swets.nl IS - 0271-3683 MH - Epinephrine iontophoresis MH - Hsv-1 reactivation MH - Subtraction cloning. MH - Herpes-simplex-virus MH - Reactivation MH - Type-1 MH - Transcription MH - Infection MH - Induction MH - Infiltration MH - Neurons MH - Cornea. AB - Purpose. The aim of the present study was to identify the genes that express in the nervous system when herpes simplex virus type 1 (HSV-1) reactivates from latency by using subtraction cloning following epinephrine iontophoresis. Methods. The corneas of New Zealand White rabbits (2 to 2.5 kg) were topically inoculated with 5 x 10(5) PFU of plaque-purified HSV-1 strain McKrae. Corneal infection was monitored by slit lamp examination (SLE) on days 3-7 after infection. At 30 d post-infection, eyes were assessed for infectious virus by ocular swabs. Disappearance of virus in ocular swabs signified latency for HSV-1. Latently-infected rabbits were subjected to transcorneal iontophoresis. The messenger RNA (mRNA) was isolated from the trigeminal ganglia and used for subtraction cloning and subtraction library synthesis. Results. Our results indicate 18 genes with increased expression and 6 genes with decreased levels. Northern blot analysis revealed that genes for OX-40 ligand, MHC class II HLA-DR-alpha and beta-microglobulin showed predominant increase followed by tyrosine 3'-monooxygenase, proteolipid protein, tyrosine beta, and apolipoprotein D. Genes that underwent reduced expression included cytochrome c oxidase subunit I, ATP synthase isoform, cytochrome b, ATPase 9, and at least one unidentified gene. Conclusions. Our results, particularly the increased detection of OX-40 ligand and MHC class II-alpha and beta-2 microglobulin, support the finding that lymphocytes persist in latently-infected trigeminal ganglia (TGs). These results also suggest a role of the immune system in HSV-1 recurrences. In addition, we have detected genes associated with neuronal disorders, OX-40L, proteolipid protein, and apolipoprotein D. [References: 22] LG - English PT - Article SB - Current Contents(R)/Life Sciences CC - Medical Research, Organs & Systems in Current Contents(R)/Life Sciences. EW - 2003 week 33 IN - Reprint available from: Kwon BS Univ Ulsan, Immunomodulat Res Ctr Ulsan 680749 South Korea Univ Ulsan, Immunomodulat Res Ctr Ulsan 680749 South Korea Louisiana State Univ, Ctr Eye New Orleans, LA 70112 USA <27> UI - 702VP-0002 DD - ISI Document Solution: 702VP AU - Haddadin MH AU - Hawkins AL AU - Long P AU - Morsberger LA AU - Depew D AU - Epstein JI AU - Griffin CA MA - cgriffin@jhmi.edu RA - Griffin CA TI - Cytogenetic study of malignant triton tumor: a case report SO - Cancer Genetics & Cytogenetics. 144(2):100-105, 2003 Jul 15. AS - Cancer Genet. Cytogenet 2003 Jul 15;144(2):100-105 PU - ELSEVIER SCIENCE INC, 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA. URL: http://www.elsevier.nl IS - 0165-4608 MH - Nerve-sheath tumors MH - Wiedemann-beckwith syndrome MH - Cell nuclear antigen MH - Molecular analysis MH - Expression MH - Mutations MH - Gene MH - P53 MH - Rhabdomyosarcoma MH - Differentiation. AB - Malignant triton tumor (MTT) is a highly malignant neoplasm, classified as a variant of malignant peripheral nerve sheath tumor (MPNST) with rhabdomyoblastic differentiation. Few cytogenetic studies of MTT have been reported using conventional cytogenetic analysis. Here, we report a comprehensive cytogenetic study of a case of MTT using G-banding, Spectral Karyotyping(TM), and fluorescence in situ hybridization (FISH) for specific regions. A complex hyperdiploid karyotype with multiple unbalanced translocations was observed: 48similar to55, XY, der(7)add(7)(p?)dup(7)[2], der(7) t(7;20)(p22;?)ins(20;19)[5], der(7)ins(8;7)(?;p22q36)t(3;8)t(8;20)[15],-8[5],-8[19],r(8)dup(8), +der(8)r(8;22)[4],-9[9],der(II)t(11;20)(p15;?)ins(20;19)[22],der(12) t(8;12)(q21;p13)[21],der(13) t(3;13)(q25;p11),-17,-19,der(19)t(17;19)(q11.2;q13.1),-20,-22,+4-7r[ cp24]/46,XY[13]. The 1995 International System for Human Cytogenetic Nomenclature was followed where possible. Note that breakpoints were frequently omitted where only SKY information was known for a small part of an involved chromosome. Our analysis revealed some breakpoints in common with those previously reported in MTT, MPNST, and rhabdomyosarcoma, namely 7p22, 7q36, 11p15, 12p13, 13p11.2, 17q11.2, and 19q13.1. FISH showed high increase of copy number for MYC and loss of a single copy for TP53. (C) 2003 Elsevier Inc. All rights reserved. [References: 38] LG - English PT - Article SB - Current Contents(R)/Life Sciences CC - Oncogenesis & Cancer Research in Current Contents(R)/Life Sciences. EW - 2003 week 33 IN - Reprint available from: Griffin CA Johns Hopkins Univ, Sch Med, Dept Pathol 600 N Wolfe St,Carnegie 367 Baltimore, MD 21287 USA Johns Hopkins Univ, Sch Med, Dept Pathol Baltimore, MD 21287 USA Johns Hopkins Univ, Sch Med, Dept Oncol Baltimore, MD 21287 USA <28> UI - 701GE-0005 DD - ISI Document Solution: 701GE AU - Shieh WY AU - Jean WD AU - Lin YT AU - Tseng M MA - winyang@ms.cc.ntu.edu.tw RA - Shieh WY TI - Marinobacter lutaoensis sp nov., a thermotolerant marine bacterium isolated from a coastal hot spring in Lutao, Taiwan SO - Canadian Journal of Microbiology. 49(4):244-252, 2003 Apr. AS - Can. J. Microbiol 2003 Apr;49(4):244-252 PU - NATL RESEARCH COUNCIL CANADA, RESEARCH JOURNALS, MONTREAL RD, OTTAWA, ONTARIO K1A 0R6, CANADA. URL: http://www.nrc.ca/cisti/journals/ IS - 0008-4166 MH - Marinobacter lutaoensis MH - Thermotolerant bacteria MH - Halophile MH - Marine bacteria. MH - Gen-nov MH - Thermophilic eubacteria MH - Anaerobic bacterium MH - Cell-walls MH - Comb. nov MH - Represents MH - Sea MH - Denitrification MH - Thermotogales MH - Spirochaeta. AB - A heterotrophic and thermotolerant marine bacterium, designated strain T5054, was isolated from a hot spring on the coast of Lutao, Taiwan. It was a strictly aerobic, Gram-negative rod. Cells grown in broth cultures were non-spore-forming and motile by means of one to several polar flagella. It seems that pilus-like structures were produced from both poles of the cells. Strain T5054 required Na+ for growth and exhibited optimal growth at about 45degreesC, pH 7, and 3-5% NaCl. It contained iso-C-15:0 as the most abundant fatty acid and ubiquinone-8 as the only isoprenoid quinone. Its genomic DNA G+C content was 63.5 mol%. The strain did not require vitamins or other organic growth factors, and it grew on glucose, mannitol, and a variety of organic acids and amino acids as sole carbon sources. Characterization data, together with the results of a 16S rDNA-based phylogenetic analysis, indicated that strain T5054 could be classified as a new species in the genus Marinobacter. The name Marinobacter lutaoensis sp. nov. is proposed for this new bacterium. The type strain is T5054 (CCRC 17087; JCM 11179). [References: 58] LG - English PT - Article SB - Current Contents(R)/Agriculture, Biology & Environmental Sciences Current Contents(R)/Life Sciences CC - Biotechnology & Applied Microbiology in Current Contents(R)/Agricultural, Biology & Environmental Sciences. Microbiology in Current Contents(R)/Life Sciences. EW - 2003 week 33 IN - Reprint available from: Shieh WY Natl Taiwan Univ, Inst Oceanog POB 23-13 Taipei 10764 Taiwan Natl Taiwan Univ, Inst Oceanog Taipei 10764 Taiwan Food Ind Res & Dev Inst, Culture Collect & Res Ctr Hsinchu Taiwan <29> UI - 701GG-0018 DD - ISI Document Solution: 701GG AU - Polidori MC AU - Mecocci P AU - Stahl W AU - Sies H MA - polidori@uni-duesseldorf.de RA - Polidori MC TI - Cigarette smoking cessation increases plasma levels of several antioxidant micronutrients and improves resistance towards oxidative challenge SO - British Journal of Nutrition. 90(1):147-150, 2003 Jul. AS - Br. J. Nutr 2003 Jul;90(1):147-150 PU - C A B I PUBLISHING, C/O PUBLISHING DIVISION, WALLINGFORD OX10 8DE, OXON, ENGLAND. URL: http://www.cabi.org/ IS - 0007-1145 MH - Smoking MH - Antioxidants MH - Micronutrients MH - Oxidative stress. MH - Human-blood-plasma MH - Stroke patients MH - Vitamin-c MH - Malondialdehyde MH - Damage MH - Disease. AB - Cigarette smoking is associated epidemiologically with increased risk of cardiovascular diseases, but the pathophysiological mechanisms are still not fully understood. There is evidence that smoking is related to increased free radical production and antioxidant depletion, but the effects of smoking cessation on plasma concentrations of antioxidants and susceptibility to oxidative stress are largely unknown. Plasma levels of vitamins A, C, E, uric acid, total thiols, carotenoids (including lutein, zeaxanthin, beta-cryptoxanthin, lycopene, alpha- and beta-carotene) and malondialdehyde (MDA, a biomarker of lipid peroxidation) were measured in fifteen healthy, normolipidaemic subjects (seven males, eight females, 35.2 (SD 2.3) years) before and 4 weeks after smoking cessation. To determine plasma resistance towards oxidative challenge, plasma was incubated for up to 5 h with the peroxyl radical-generator 2,2'-azobis(2-amidinopropane) (AAPH); MDA and ascorbate levels were measured at various time points. The concentrations of all plasma antioxidants were lower before smoking cessation than afterwards; NIDA levels were higher before than after termination of smoking. Upon AAPH exposure, the consumption of plasma ascorbate and the production of MDA occurred at a significantly faster rate before smoking cessation as compared with after-wards. Cigarette smoking cessation is followed by a marked increase in plasma antioxidant concentrations and substantially improves plasma resistance towards oxidative challenge. Given the importance of cigarette smoking as a risk factor for cardiovascular diseases and the pathophysiological role played by oxidative stress in these illnesses, quitting smoking represents an irreplaceable preventive strategy against tobacco-induced oxidative stress and vascular damage. [References: 22] LG - English PT - Article SB - Current Contents(R)/Agriculture, Biology & Environmental Sciences Current Contents(R)/Life Sciences CC - Food Science/Nutrition in Current Contents(R)/Agricultural, Biology & Environmental Sciences. Endocrinology, Nutrition & Metabolism in Current Contents(R)/Life Sciences. EW - 2003 week 33 IN - Reprint available from: Polidori MC Univ Dusseldorf, Inst Biochem & Mol Biol 1 POB 101007 D-40001 Dusseldorf Germany Univ Dusseldorf, Inst Biochem & Mol Biol 1 D-40001 Dusseldorf Germany Perugia Univ Hosp, Inst Gerontol & Geriatr Perugia Italy <30> UI - 702TP-0001 DD - ISI Document Solution: 702TP AU - Keller SH AU - Nigam SK MA - shkeller@ucsd.edu RA - Keller SH TI - Biochemical processing of E-cadherin under cellular stress SO - Biochemical & Biophysical Research Communications. 307(2):215-223, 2003 Jul 25. AS - Biochem. Biophys. Res. Commun 2003 Jul 25;307(2):215-223 PU - ACADEMIC PRESS INC ELSEVIER SCIENCE, 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA. URL: http://www.apnet.com IS - 0006-291X MH - Caspase MH - Mdck cells MH - Antimycin MH - Deoxyglucose MH - Cellular stress MH - Ischemia MH - Adherens junction MH - E-cadherin MH - Protein degradation MH - Apoptosis. MH - Staurosporine-induced apoptosis MH - Respiratory-chain inhibitors MH - Cytochrome-c MH - Caspase-3-dependent cleavage MH - Adherens junctions MH - Caspase inhibitors MH - Atp depletion MH - Death MH - Cells MH - Release. AB - The proteolytic cleavage pathways of E-cadherin endogenously expressed in MDCK (Madin-Darby canine kidney) cells were characterized in cells treated with antimycin A and deoxyglucose to examine transmembrane protein processing under cellular stress. E-cadherin is a type I transmembrane protein which operates as the cell adhesion molecule component of the adherens junction, a complex of proteins involved in epithelial tissue development and integrity. We now demonstrate that treatment of MDCK cells with antimycin A and deoxyglucose activates caspase mediated pathways that cleave E-cadherin. E-cadherin is cleaved into two major fragments. with the sizes predicted by the location of a caspase-3 cleavage consensus sequence. Cleavage of E-cadherin and deposition of the C-terminal fragment into the cytoplasm are inhibited by the caspase inhibitor DEVD-CHO. Thus, a major mechanism for E-cadherin cleavage and dissolution of the adherens junction under antimycin/deoxyglucose treatment is caspase mediated, initiated by activation of an apoptosis pathway. (C) 2003 Elsevier Science (USA). All rights reserved. [References: 36] LG - English PT - Article SB - Current Contents(R)/Life Sciences CC - Biochemistry & Biophysics in Current Contents(R)/Life Sciences. EW - 2003 week 33 IN - Reprint available from: Keller SH Univ Calif San Diego, Dept Med 210A Dickinson St San Diego, CA 92103 USA Univ Calif San Diego, Dept Med San Diego, CA 92103 USA Univ Calif San Diego, Dept Med & Pediat La Jolla, CA 92093 USA <31> UI - 702YJ-0002 DD - ISI Document Solution: 702YJ AU - Schroeder M AU - Barth H AU - Reuter R RA - Schroeder M TI - Effect of inelastic scattering on underwater daylight in the ocean: model evaluation, validation, and first results SO - Applied Optics. 42(21):4244-4260, 2003 Jul 20. AS - Appl. Optics 2003 Jul 20;42(21):4244-4260 PU - OPTICAL SOC AMER, 2010 MASSACHUSETTS AVE NW, WASHINGTON, DC 20036 USA. URL: http://www.osa.org IS - 0003-6935 MH - Dissolved organic-matter MH - Water-leaving radiance MH - Discrete space theory MH - Radiative-transfer MH - Raman-scattering MH - Optical-properties MH - Chlorophyll-a MH - Pure water MH - Absorption-spectrum MH - Natural-waters. AB - A model based on a matrix-operator theory capable of simulating underwater daylight in the ocean is presented. The main focus is on gelbstoff and chlorophyll fluorescence as well as water Raman scattering as sources of inelastic scattering and their effect on underwater daylight and relevance for the remote sensing of ocean color. Any combination of inelastic sources can be investigated, including differences in simulated underwater daylight in the absence and the presence of these sources. To our knowledge, it is the first matrix-operator model to include all these inelastic sources. The model allows simulations for case 1 and case 2 waters. Calculations can be done with highly anisotropic phase functions as they are observed in the ocean, and every order of multiple scattering is considered. A detailed mathematical description of inelastic sources is given, and a special treatment of the depth dependency of these sources is presented. The model is validated by comparison with depth-dependent and spectrally resolved measurements of downward irradiance in the open ocean. The differences between measured and simulated data are within the error of the radiometric measurements. Water Raman scattering has been found to contribute significantly to water-leaving radiance. The inelastic fraction depends on the water Raman scattering coefficient, on the ratio of the total attenuation coefficient at excitation and emission wavelengths, and on the spectral course of the irradiance incident on the ocean. For clear ocean waters the inelastic fraction can reach values of more than 17% [C = 0.03 mg m(-3), alpha(y) (440 nm) = 0.01 m(-1)] at wavelengths relevant for the remote sensing of ocean color. The inelastic fraction of gelbstoff fluorescence can reach or even exceed the relevance of water Raman scattering at short wavelengths. In the water column, depending on optically active substances and on actual depth, water Raman scattering can provide 100% of the light field at wavelengths greater than 580 nm. The effect of gelbstoff fluorescence on depth-dependent irradiances is less significant than the effect of water Raman scattering in all cases considered, except for near surface levels and high gelbstoff concentrations. (C) 2003 Optical Society of America. [References: 60] LG - English PT - Article SB - Current Contents(R)/Physical, Chemical & Earth Sciences Current Contents(R)/Engineering, Computing & Technology CC - Applied Physics/Condensed Matter/Materials Science in Current Contents(R)/Physical, Chemical & Earth Sciences. Optics & Acoustics in Current Contents(R)/Engineering, Computing & Technology. EW - 2003 week 33 IN - Reprint available from: Schroeder M Free Univ Berlin, Inst Weltraumwissensch Carl Heinrich Becker Weg 6-10 D-12165 Berlin Germany Free Univ Berlin, Inst Weltraumwissensch D-12165 Berlin Germany Univ Oldenburg, Fachbereich Phys D-26111 Oldenburg Germany <32> UI - 702EE-0005 DD - ISI Document Solution: 702EE AU - Yoshida E AU - Nakamura A AU - Watanabe T MA - watanabe@iis.u-tokyo.ac.jp RA - Watanabe T TI - Reversed-phase HPLC determination of chlorophyll a ' and naphthoquinones in photosystem I of red algae: Existence of two menaquinone-4 molecules in photosystem I of Cyanidium caldarium SO - Analytical Sciences. 19(7):1001-1005, 2003 Jul. AS - Anal. Sci 2003 Jul;19(7):1001-1005 PU - JAPAN SOC ANALYTICAL CHEMISTRY, 26-2 NISHIGOTANDA 1 CHOME SHINAGAWA-KU, TOKYO, 141, JAPAN. URL: http:///soc/nacsis.ac.jp/jsac/ IS - 0910-6340 MH - Light-harvesting complex MH - Porphyridium-cruentum MH - Photosynthetic pigments MH - Extinction coefficients MH - Liquid-chromatography MH - Reaction centers MH - Phylloquinone MH - Gene MH - Site MH - Purification. AB - Chlorophyll (Chl) a', the C13(2)-epimer of Chl a, is one of the two Chl molecules constituting the primary electron donor (P700) of photosystem (PS) I of a thermophilic cyanobacterium Synechococcus elongatus. To examine whether PS I of other oxygenic photosynthetic organisms in general contain one Chl a' molecule in P700, the pigment composition of thylakoid membranes and PS I preparations isolated from red algae Porphyridium purpureum and Cyanidium caldarium was examined by reversed-phase HPLC with particular attention to Chl a' and phylloquinone (PhQ), the secondary electron acceptor of PS I. The two red algae contained one Chl a' molecule at the core part of PS I. In PS I of C. caldarium, two menaquinone-4 (MQ-4) molecules were detected in place of PhQ used by higher plants and cyanobacteria. The 1:2:1 stoichiometry among Chl a', PhQ (MQ-4) and P700 in PS I of the red algae indicates that one Chl a' molecule universally exists in PS I of oxygenic photosynthetic organisms, and two MQ-4 molecules are associated with PS I of C caldarium. [References: 38] LG - English PT - Article SB - Current Contents(R)/Physical, Chemical & Earth Sciences CC - Spectroscopy/Instrumentation/Analytical Sciences in Current Contents(R)/Physical, Chemical & Earth Sciences. EW - 2003 week 33 IN - Reprint available from: Watanabe T Univ Tokyo, Inst Ind Sci, Meguro Ku Komaba Tokyo 1538505 Japan Univ Tokyo, Inst Ind Sci, Meguro Ku Tokyo 1538505 Japan <33> UI - 701DN-0041 DD - ISI Document Solution: 701DN AU - Yamamoto K AU - Sokabe T AU - Ohura N AU - Nakatsuka H AU - Kamiya A AU - Ando J MA - joji@m.u-tokyo.ac.jp RA - Ando J TI - Endogenously released ATP mediates shear stress-induced Ca2+ influx into pulmonary artery endothelial cells SO - American Journal of Physiology - Heart & Circulatory Physiology. 285(2):H793-H803, 2003 Aug. AS - Am. J. Physiol.-Heart Circul. Physiol 2003 Aug;285(2):H793-H803 PU - AMER PHYSIOLOGICAL SOC, 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA. URL: http://www.the-aps.org/ IS - 0363-6135 MH - Hemodynamic force MH - Mechanotransduction MH - Atp synthase MH - Purinoceptors MH - P2x(4) receptor. MH - Mechanical stimuli MH - Increased flow MH - Calcium MH - Receptors MH - Angiostatin MH - Modulation MH - Synthase MH - Surface. AB - The mechanisms by which flow-imposed shear stress elevates intracellular Ca2+ in cultured endothelial cells (ECs) are not fully understood. Here we report finding that endogenously released ATP contributes to shear stress-induced Ca2+ responses. Application of flow of Hanks' balanced solution to human pulmonary artery ECs (HPAECs) elicited shear stress-dependent increases in Ca2+ concentrations. Chelation of extracellular Ca2+ with EGTA completely abolished the Ca2+ responses, whereas the phospholipase C inhibitor U-73122 or the Ca2+-ATPase inhibitor thapsigargin had no effect, which thereby indicates that the response was due to the influx of extracellular Ca2+. The Ca2+ influx was significantly suppressed by apyrase, which degrades ATP, or antisense oligonucleotide targeted to P2X(4) purinoceptors. A luciferase luminometric assay showed that shear stress induced dose-dependent release of ATP. When the ATP release was inhibited by the ATP synthase inhibitors angiostatin or oligomycin, the Ca2+ influx was markedly suppressed but was restored by removal of these inhibitors or addition of extracellular ATP. These results suggest that shear stress stimulates HPAECs to release ATP, which activates Ca2+ influx via P2X(4) receptors. [References: 29] LG - English PT - Article SB - Current Contents(R)/Life Sciences CC - Cardiovascular & Hematology Research in Current Contents(R)/Life Sciences. EW - 2003 week 33 IN - Reprint available from: Ando J Univ Tokyo, Grad Sch Med, Dept Biomed Engn, Bunkyo Ku 7-3-1 Hongo Tokyo 1130033 Japan Univ Tokyo, Grad Sch Med, Dept Biomed Engn, Bunkyo Ku Tokyo 1130033 Japan Nihon Univ, Interdisciplinary Sci Ctr Tokyo 1130033 Japan <34> UI - 702BU-0004 DD - ISI Document Solution: 702BU AU - Eto K AU - Yamashita T AU - Hirose K AU - Tsubamoto Y AU - Ainscow EK AU - Rutter GA AU - Kimura S AU - Noda M AU - Iino M AU - Kadowaki T MA - kadowaki-3im@h.u-tokyo.ac.jp RA - Kadowaki T TI - Glucose metabolism and glutamate analog acutely alkalinize pH of insulin secretory vesicles of pancreatic beta-cells SO - American Journal of Physiology - Endocrinology & Metabolism. 285(2):E262-E271, 2003 Aug. AS - Am. J. Physiol.-Endocrinol. Metab 2003 Aug;285(2):E262-E271 PU - AMER PHYSIOLOGICAL SOC, 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA. URL: http://www.the-aps.org/ IS - 0193-1849 MH - Glutamate metabolism MH - Alkalinization of vesicular ph MH - Regulation of insulin secretion. MH - Nadh shuttle system MH - Cytoplasmic ph MH - Cytosolic ca2+ MH - Hyperinsulinism/hyperammonemia syndrome MH - Regulating exchangers MH - Ketoisocaproic acid MH - Dehydrogenase gene MH - Anesthetized rats MH - Synaptic vesicles MH - Dimethyl ester. AB - We studied acute changes of secretory vesicle pH in pancreatic beta-cells with a fluorescent pH indicator, lysosensor green DND-189. Fluorescence was decreased by 0.66 +/- 0.10% at 149 +/- 16 s with 22.2 mM glucose stimulation, indicating that vesicular pH was alkalinized by similar to 0.016 unit. Glucose-responsive pH increase was observed when cytosolic Ca2+ influx was blocked but disappeared when an inhibitor of glycolysis or mitochondrial ATP synthase was present. Glutamate dimethyl ester (GME), a plasma membrane-permeable analog of glutamate, potentiated glucose-stimulated insulin secretion at 5 mM without changing cellular ATP content or cytosolic Ca2+ concentration ([Ca2+]). Application of GME at basal glucose concentration decreased DND-189 fluorescence by 0.83 +/- 0.19% at 38 +/- 2 s. These results indicated that the acutely alkalinizing effect of glucose on beta-cell secretory vesicle pH was dependent on glucose metabolism but independent of modulations of cytosolic [Ca2+]. Moreover, glutamate derived from glucose may be one of the mediators of this alkalinizing effect of glucose, which may have potential relevance to the alteration of secretory function by glutamate. [References: 57] LG - English PT - Article SB - Current Contents(R)/Life Sciences CC - Endocrinology, Nutrition & Metabolism in Current Contents(R)/Life Sciences. EW - 2003 week 33 IN - Reprint available from: Kadowaki T Univ Tokyo, Grad Sch Med, Dept Metab Dis, Bunkyo Ku 7-3-1 Hongo Tokyo 1138655 Japan Univ Tokyo, Grad Sch Med, Dept Metab Dis, Bunkyo Ku Tokyo 1138655 Japan Univ Tokyo, Grad Sch Med, Dept Pharmacol Tokyo 1138655 Japan Japan Sci & Technol Corp, CREST Saitama 3320012 Japan Asahi Life Fdn, Inst Diabet Care & Res Tokyo 1000005 Japan Univ Bristol, Sch Med Sci, Dept Biochem Bristol BS8 1TD Avon England <35> UI - 703KJ-0008 DD - ISI Document Solution: 703KJ AU - Czerpak R AU - Piotrowska A AU - Dobrzyn P AU - Tatur A AU - Marczuk M RA - Czerpak R TI - The effect of biochanin A on the chlorophylls and carotenoids content in the alga Chlorella vulgaris Beijerinck SO - Acta Physiologiae Plantarum. 25(3):271-278, 2003. AS - Acta Physiol. Plant 2003;25(3):271-278 PU - POLISH ACAD SCIENCES DEPT PLANT PHYSIOLOGY, SLAWKOWSKA STREET NO 17, PL31-016 KRAKOW, POLAND IS - 0137-5881 MH - Chlorella vulgaris MH - Biochanin a MH - Growth MH - Chlorophylls a and b MH - Carotenes MH - Xanthophylls. MH - Steroidal estrogens MH - Xanthophyll cycle MH - Higher-plants MH - Cell-line MH - Genistein MH - Inhibition MH - Hormones MH - Daidzein MH - Growth. AB - The present study was undertaken to determine the influence of biochanin A, isoflavone characterised by estrogenic activity, upon the content of chlorophylls and carotenoids in the cells of green alga Chlorella vulgaris Beijerinck (Chlorophyceae). On the 6(th) day of cultivation under the influence of 10(-6) M biochanin A exerted the greatest biological activity and the most stimulating effect on the analysed parameters: growth of the alga expressed by the cells number and the content of photosynthetic pigments in them. The total content of carotenoids was stimulated on the 6(th) day of experiment in the range of 197 % but during the 9(th) day only in 179 % in comparision with the control group (100 %). At the same time content of carotenes increased to the level of 123 - 119 % and xanthophylls to 208 - 178 %. Among the carotenes, beta-carotene was characterised with the 3.7 times higher content in regard to the content of a-carotene on the 6 th day of cultivation and during the 9th day the 5.7 times domination. The content of xanthophylls that contain two atoms of oxygene in molecule (oxygen - poor xanthophylls) was intensively stimulated in the range of 224 %. Moreover, the oxygen - rich xanthophylls content reached the value 179 % when compared to the control. The greatest stimulation of the content of chlorophylls and its isomers was observed during the 3(rd) day of cultivation of Chlorella vulgaris when it rose up to 166 % and to 156 % on the 6(th) day. The content of chlorophyll b and its isomers was stimulated in 181 % on the 6(th) day of culture and 155 % during the 9(th) day of algal culture. The evidence on the stimulating effect of biochanin A as the main representative of isoflavones on the growth and content photosynthetic pigments in eucaryotic alga C. vulgaris was demonstrated in these studies. [References: 33] LG - English PT - Article SB - Current Contents(R)/Agriculture, Biology & Environmental Sciences CC - Plant Sciences in Current Contents(R)/Agricultural, Biology & Environmental Sciences. EW - 2003 week 33 IN - Reprint available from: Czerpak R Univ Bialystok, Inst Biol Swierkowa 20 B PL-15950 Bialystok Poland Univ Bialystok, Inst Biol PL-15950 Bialystok Poland Polish Acad Sci, Inst Ecol PL-05092 Dziekanow Lesny Lomianki Poland