Beckman
Institute for Advanced Science and Technology * UIUC*
Urbana
IL
61801
Email:
aditidas@uiuc.edu
Website:
www.life.uiuc.edu/~aditidas
PRIMARY
RESEARCH INTERESTS
Protein
Biochemistry
Bio-electrochemistry
Protein
Engineering
Surface
Fabrication
Biosensing
based on Localized Surface Plasmon Resonance
EDUCATION
Princeton
University Ph.D (Chemistry ) MA (Chemistry) 2001-2005
I.
I. T (Indian
Institute of Technology,
Kanpur)
M.Sc.
(Chemistry) 1996-1998
St.
Stephen s College,
Delhi University, India, B.Sc. (Chemistry) , 1993-1996
RESEARCH
EXPERIENCE
University of Illinois Urbana
Champaign
POST-DOC
2005-Present Mentor:
Prof. Stephen G. Sligar
Detection
of molecular recognition events to membrane proteins in Nanodisc using
Localized Surface Plasmon Resonance Spectroscopy (LSPR) and Redox Studies of
cytochrome P450s.
Princeton
University
Research
Associate 2001-2005
Mentor:
Prof. Michael H. Hecht
Thesis:
Electrochemical
and Functional Studies of De Novo Alpha Helical Protein from a Designed
Combinatorial Library.
Naval
Research Laboratory, Washington-DC
Summer
Work - 2002, 2004 Mentor:
Dr. Scott. A. Trammell
Design
of Biosensors using De Novo
Proteins as scaffolds.
Indian
Institute of Technology Delhi
Council of Scientific and Industrial Research Fellow, Biomed. Eng, 1998-2001 Mentor:
Prof. Alok. R. Ray
Synthesis
of copolymers of poly (propylene fumarate) for use bone tissue
engineering.
Indian
Institute of Technology, Kanpur
Chemistry
Department, 1996-1998, Mentor: Prof.
Sabyasachi. Sarkar
Thesis:
Model Chemistry of Aerobic Acetylene Hydratase Enzymes
AWARDS
AND HONORS
1.
Outstanding
Researcher Award, NSF-Nanoscale Science and Engineering Center
2007
2.
NSF-Nanoscale Science and Engineering
Center
(NSEC) Support 2005-2010
3.
FMC Graduate Fellowship in
Chemistry,Princeton (Top Graduate Student in
Biochemistry and Organic Chemistry) 2005
4.
Dean s List Finalist for
Honorific
Fellowship, Princeton 2004
5.
Honorific Fellowship
Nomination, Princeton (Top three in the Graduating Class of
the Chemistry Department) 2004
6.
First Year Science and Engineering Fellowship, Princeton 2001.
7.
Council of Scientific and Industrial Research (CSIR-JRF) Fellowship,India 1998
(Top 12 in India in
the field of chemistry)
8.
Merit List Scholarship in Undergraduate Studies (Top 10 out of 900
undergraduates in Chemistry in Delhi
University) 1993-1996
9.
Certificate of Merit at
Indian National Mathematics Olympiad (INMO) 1992
10.
National
Scholarship from Govt. of India, 1990
PUBLICATIONS
AND PATENTS
1.
Redox Potential Control by Drug Binding to Cytochrome P450 3A4
Aditi
Das, Yelena Grinkova and Stephen G Sligar
J.
Am. Chem. Soc.
2007 129
(45
),
13778
-
13779
2.
Peroxidase Activity of De Novo Heme Proteins Immobilized on Electrodes
Aditi
Das and Michael H. Hehcht
J.
Inorg. Biochem 2007 101 (11-12) 1820-1826
3.
Electron Transfer between P450cin and its FMN-containing Redox Partner
Novelle
Kimmich, Aditi Das, Irina Sevrioukova, Yergalem Meharenna,
Stephen G. Sligar, and Thomas L. Poulos
J.
Biol. Chem. 2007 282 (37), 27006-27011
4.
Resonance Surface Plasmon
Spectroscopy: Low Molecular Weight Substrate Binding to Cytochrome P450
Jing Zhao*,
Aditi Das*, Xiaoyu Zhang, George Schatz, Stephen Sligar, Richard Van
Duyne
*
Equal Authors
J.
Am.
Chem.
Soc.
2006 128 (34),11004-11005.
(In
News):
Enhanced LSPR detects binding of small molecules. Analytical
Chemistry 2006, Volume 78, 21, 7356.
5.
Resonance
Surface Plasmon Spectroscopy: Low Molecular Weight Substrate Binding to
Cytochrome P450: Patent Applied 2006
Richard
Van Duyne, Jing Zhao, Aditi Das, Xiaoyu Zhang, George Schatz, Stephen
Sligar
6.
Electrochemical and Binding Studies of an immobilized De
Novo Heme Protein
Aditi Das, Scott A. Trammell and Michael H. Hecht
Biophy.
Chem. 2006 123
(2-3),102-112.
7.
Nanografting De Novo Proteins onto
gold surface
Ying Hu, Aditi Das, Michael H. Hecht, and Giacinto Scoles
Langmuir
2005,
21(20),9103-09.
8.
De Novo Proteins from Designed Combinatorial Libraries.
Michael.H.Hecht,
Aditi
Das
, Abigail Go, Luke Bradley, Yinan
Wei.
Protein Science 2004,13,1711-23.
9.
Electrochemical and Functional Studies of De Novo Alpha Helical Protein from
a Designed Combinatorial Library.
Aditi
Das
Ph.D
Thesis Princeton University
November 2005
10.
Model
Chemistry of Aerobic Acetylene Hydratase Enzymes.
Aditi
Das
Masters Thesis: I.I.T (Kanpur)
1998
Manuscripts
and work in progress:
11. Resonance Surface Plasmon Spectroscopy:
The opposing effect of substrate and inhibitor binding to Cytochrome P450:
Imidazole and camphor. In Preparation
12.
Detection
of Drug binding to Cytochrome P450-3A4 nanodisc using LSPR. Work in progress
13.
Electron
Transfer Kinetics of Nitric Oxide Synthase. Work in progress
14.
CYP3A4-CPR
in membranes Work in
progress.
15. Small molecule binding
by a cavity forming mutant of a De Novo
designed protein: Screening for binding and determination of binding constant
using NMR. In preparation.
16.
Femotosecond correlation spectroscopy to detect the signature pattern of P450
proteins. Work in Progress.
CONFERENCE
TALKS AND POSTERS (SELECTED)
1.
Membrane Protein
Biorecognition Events in Nanodiscs. NSF
site visit at the NSF Nanoscale Science & Engineering Center (NSEC) for
Integrated Nanopatterning and Detection Technologies and Signal Transduction
and Receptor Design SRG. 2007 POSTER
2.
Ultra-sensitive
Assays of Biorecognition Events in Membrane Proteins and Receptors
Annual
Meeting at NSFNanoscaleScience & Engineering Center
(NSEC) for Integrated Nanopatterning and Detection Technologies 2006 POSTER
3.
Assays
of Pattermed Membrane Protein and Receptors via Nanodiscs. Annual Meeting at NSF
Nanoscale Science & Engineering Center (NSEC)
for Integrated Nanopatterning and Detection Technologies 2006 POSTER
4.
Direct Assembly of De Novo Proteins on Gold Surfaces using
Nanografting Seeing at the nanoscale Conferece at Univ. of Pennsylvania July
17-20, 2006 POSTER
5.
Electrochemical
Studies and Biosensor Applications of a De
Novo Designed Heme Binding Proteins. 17th
Symposium of the Protein Society, Boston MA,
July
26-30, 2003.
Programs and Abstracts. Protein Science Vol 12, Supplement 2, July 2003.
TALK
6.
De
Novo Designed Heme Binding Proteins
Immobilized on Gold Surface: Electrochemical Studies and Biosensor
Applications. Abstracts
of Papers, 228th
ACS NationalMeeting, Philadelphia,PA,August
22-26, 2004 in Philadelphia.
Inorganic Division. TALK
7.
De Novo Proteins as Biosensors
Chemical and
Biological Engineering. Rensselaer Polytechnic
Institute (RPI).
TALK
8.
Combinatorially Designed De novo proteins: Immobilization on gold and applications as
biosensors. Abstracts
of Papers, 228th ACS National Meeting, Philadelphia PA, August
22-26, 2004
in Philadelphia.
Biological Chemistry Division. POSTER
9.
Developments in the synthesis of
Biodegradable/Biocompatible Polymers using poly(propylene fumarate). National
Symposium of Biomaterials and Artificial organs, Indian Institute of Technology,
Kharagpur, December
6-8, 2000
India.
TALK
TEACHING
AND MENTORING EXPERIENCE
Teaching
Assistant in General Chemistry Labs (CHM 201)
in Fall 2002-2004 Obtained Average evaluation of (4.5/5) = Excellent
Mentored
three junior graduate students and two senior undergraduate students during
research associate position in Princeton University
REFERENCES
Prof.
Stephen G. Sligar, University of Illinois - Urbana Champaign
Prof.
Michael H. Hecht, Princeton University
Prof.
Giacinto Scoles, Princeton University and International School for Advanced
Studies of Trieste.
Prof.
Andrew Bocarsly, Princeton University
Prof.
Richard Van Duyne, Northwestern University
Dr.
Scott A Trammell, Naval Research Lab, Washington DC.
